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Antibody-Dependent THP-1 Cell-Mediated Phagocytosis of Plasmodium falciparum-Infected Erythrocytes

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Malaria Immunology

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2470))

Abstract

Antibodies that recognize variant surface antigens (VSAs) expressed on Plasmodium falciparum-infected erythrocytes (IEs) opsonize IEs for phagocytic clearance. The anti-VSA antibodies promote antibody-dependent cellular phagocytosis (ADCP) of IEs by interacting with innate immune cells. ADCP is an important immune effector mechanism of parasite clearance. ADCP can be a tool to assess the efficacy of vaccine-induced antibodies, in addition to measuring the neutralizing ability of antibodies. Here, we developed and validated an efficient and high-throughput plate-based flow cytometric assay to measure ADCP of IEs using the human monocytic THP-1 cell line. This flow cytometric assay can be used to analyze the level of naturally acquired or vaccine-induced opsonic antibodies in large cohorts.

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Authors and Affiliations

  1. Department of Medicine, The Doherty Institute, The University of Melbourne, Melbourne, VIC, Australia

    Meseret Workineh Kassa, Wina Hasang & Stephen John Rogerson

Authors
  1. Meseret Workineh Kassa
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  2. Wina Hasang
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  3. Stephen John Rogerson
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Corresponding author

Correspondence to Stephen John Rogerson .

Editor information

Editors and Affiliations

  1. Centre for Medical Parasitology, Department of Immunology and Microbiology, University of Copenhagen, Copenhagen, Denmark

    Anja Tatiana Ramstedt Jensen

  2. Centre for Medical Parasitology, Department of Immunology and Microbiology, University of Copenhagen, Copenhagen, Denmark

    Lars Hviid

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© 2022 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature

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Kassa, M.W., Hasang, W., Rogerson, S.J. (2022). Antibody-Dependent THP-1 Cell-Mediated Phagocytosis of Plasmodium falciparum-Infected Erythrocytes. In: Jensen, A.T.R., Hviid, L. (eds) Malaria Immunology. Methods in Molecular Biology, vol 2470. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2189-9_46

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  • DOI: https://doi.org/10.1007/978-1-0716-2189-9_46

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-2188-2

  • Online ISBN: 978-1-0716-2189-9

  • eBook Packages: Springer Protocols

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