Abstract
Recording neural activity from multiple brain regions allows researchers to investigate the dynamic interactions between connected regions within a brain circuit. Pairing multisite recordings with circuit manipulation and behavior is a powerful approach that allows us to address hypotheses about the precise mechanisms underlying complex behaviors. There are, however, many technical challenges to each of these research methods, and feasibility issues can hamper progress especially when the three techniques are combined in one experiment. This chapter will detail the methods employed in my laboratory for performing neural recordings in conjunction with circuit manipulation and behavior. My hope is that this chapter will provide some guidance on basic good practices that can be scaled to many specific applications and adapted to incorporate new technological advances as they emerge.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Similar content being viewed by others
References
Whishaw IO, Kolb B (2004) The behavior of the laboratory rat: a handbook with tests. Oxford university Press, New York
Buzsaki G, Leung LW, Vanderwolf CH (1983) Cellular bases of hippocampal EEG in the behaving rat. Brain Res 287(2):139–171. https://doi.org/10.1016/0165-0173(83)90037-1
Ylinen A, Bragin A, Nadasdy Z, Jando G, Szabo I, Sik A, Buzsaki G (1995) Sharp wave-associated high-frequency oscillation (200 Hz) in the intact hippocampus: network and intracellular mechanisms. J Neurosci 15(1 Pt 1):30–46
Sullivan D, Mizuseki K, Sorgi A, Buzsaki G (2014) Comparison of sleep spindles and theta oscillations in the hippocampus. J Neurosci 34(2):662–674. https://doi.org/10.1523/JNEUROSCI.0552-13.2014
Hallock HL, Wang A, Griffin AL (2016) Ventral midline thalamus is critical for hippocampal-prefrontal synchrony and spatial working memory. J Neurosci 36(32):8372–8389. https://doi.org/10.1523/JNEUROSCI.0991-16.2016
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2022 Springer Science+Business Media, LLC, part of Springer Nature
About this protocol
Cite this protocol
Griffin, A.L. (2022). Multisite Recording During Memory-Guided Behavior. In: Vertes, R.P., Allen, T. (eds) Electrophysiological Recording Techniques. Neuromethods, vol 192. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2631-3_8
Download citation
DOI: https://doi.org/10.1007/978-1-0716-2631-3_8
Published:
Publisher Name: Humana, New York, NY
Print ISBN: 978-1-0716-2630-6
Online ISBN: 978-1-0716-2631-3
eBook Packages: Springer Protocols