Abstract
SiRNA induced gene silencing or RNA interference is a powerful tool to knock down gene expression and perform gene function studies. In this chapter, we describe a basic method to silence gene expression by transfecting a specific synthetic siRNA into mammalian HeLa cells.
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References
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Acknowledgment
This work was supported by the Centre National pour la Recherche Scientifique, the Université de Lorraine, the Région Lorraine and the Ligue nationale pour la recherche contre le cancer (comités 54 et 57).
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Abel, Y., Rederstorff, M. (2015). Gene Expression Knockdown by Transfection of siRNAs into Mammalian Cells. In: Rederstorff, M. (eds) Small Non-Coding RNAs. Methods in Molecular Biology, vol 1296. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-2547-6_18
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DOI: https://doi.org/10.1007/978-1-4939-2547-6_18
Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-2546-9
Online ISBN: 978-1-4939-2547-6
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