SIPA1L2 controls trafficking and local signaling of TrkB-containing amphisomes at presynaptic terminals

Andres-Alonso, Maria; Ammar, Mohamed Raafet; Butnaru, Ioana; Gomes, Guilherme M.; Acuña Sanhueza, Gustavo; Raman, Rajeev; Yuanxiang, **An; Borgmeyer, Maximilian; Lopez-Rojas, Jeffrey; Raza, Syed Ahsan; Brice, Nicola; Hausrat, Torben J.; Macharadze, Tamar; Diaz-Gonzalez, Silvia; Carlton, Mark; Failla, Antonio Virgilio; Stork, Oliver; Schweizer, Michaela; Gundelfinger, Eckart D.; Kneussel, Matthias; Spilker, Christina; Karpova, Anna; Kreutz, Michael R.

doi:10.1038/s41467-019-13224-z

SIPA1L2 controls trafficking and local signaling of TrkB-containing amphisomes at presynaptic terminals

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Published: 29 November 2019

Volume 10, article number 5448, (2019)
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SIPA1L2 controls trafficking and local signaling of TrkB-containing amphisomes at presynaptic terminals

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Maria Andres-Alonso^1,2,
Mohamed Raafet Ammar²,
Ioana Butnaru²,
Guilherme M. Gomes ORCID: orcid.org/0000-0001-8566-4284²,
Gustavo Acuña Sanhueza²,
Rajeev Raman²,
**An Yuanxiang²,
Maximilian Borgmeyer¹,
Jeffrey Lopez-Rojas²,
Syed Ahsan Raza³,
Nicola Brice⁴,
Torben J. Hausrat⁵,
Tamar Macharadze²,
Silvia Diaz-Gonzalez¹,
Mark Carlton⁴,
Antonio Virgilio Failla⁶,
Oliver Stork^3,7,
Michaela Schweizer⁸,
Eckart D. Gundelfinger ORCID: orcid.org/0000-0001-9377-7414^7,9,10,
Matthias Kneussel⁵,
Christina Spilker ORCID: orcid.org/0000-0002-7996-7673²,
Anna Karpova^2,7 &
…
Michael R. Kreutz^1,2,7

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Abstract

Amphisomes are organelles of the autophagy pathway that result from the fusion of autophagosomes with late endosomes. While biogenesis of autophagosomes and late endosomes occurs continuously at axon terminals, non-degradative roles of autophagy at boutons are barely described. Here, we show that in neurons BDNF/TrkB traffick in amphisomes that signal locally at presynaptic boutons during retrograde transport to the soma. This is orchestrated by the Rap GTPase-activating (RapGAP) protein SIPA1L2, which connects TrkB amphisomes to a dynein motor. The autophagosomal protein LC3 regulates RapGAP activity of SIPA1L2 and controls retrograde trafficking and local signaling of TrkB. Following induction of presynaptic plasticity, amphisomes dissociate from dynein at boutons enabling local signaling and promoting transmitter release. Accordingly, sipa1l2 knockout mice show impaired BDNF-dependent presynaptic plasticity. Taken together, the data suggest that in hippocampal neurons, TrkB-signaling endosomes are in fact amphisomes that during retrograde transport have local signaling capacity in the context of presynaptic plasticity.

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Introduction

BDNF/TrkB signaling is required for a variety of neuronal functions including neurotransmission and synaptic plasticity. Upon binding to BDNF, BDNF/TrkB is internalized via pinocytosis^1,2, or in a clathrin-dependent manner³ to vesicular compartments called signaling endosomes that might allow for local signaling. Signaling endosomes are predominantly generated at axon terminals by different mechanisms and constitute long-lived organelles that persist while undergoing transport from nerve terminals to the neuronal soma^4,5,6. In neurons, retrograde trafficking of TrkB signaling endosomes constitutes an important long-range signaling mechanism that conveys information on presynaptic activity to the cell soma⁷. How endosomal sorting is accomplished in these organelles and how they escape from the degradative pathway remains still unclear.

A key consequence of TrkB signaling is sustained ERK activation, a process achieved via activation of the small GTPase Rap1 (Supplementary Fig. 1a)⁸. Accordingly, prenylated Rap1 is associated with TrkB signaling endosomes and regulation of its GTPase activity is, therefore, a likely mechanism to control local TrkB signaling.

A regulator of Rap1 is SIPA1L2 (also known as SPAR2), a member of the SIPA1L family of neuronal RapGAPs (Supplementary Fig. 1b–d)⁹. The protein is most abundant in granule cells of the dentate gyrus (DG) and cerebellum and shows RapGAP activity for Rap1 and 2¹⁰. This RapGAP activity promotes the intrinsic GTPase activity of Rap1/2 that catalyzes the hydrolysis of GTP to GDP and inactivates Rap1/2 and consequently, ERK signaling. Here, we report that sipa1l2 knockout (ko) mice show impaired long-term potentiation (LTP) at mossy fiber (MF) synapses and spatial pattern separation, which requires MF plasticity. MF-LTP is an NMDA receptor-independent form of LTP that is expressed presynaptically and depends upon local BDNF/TrkB signaling¹¹. Accordingly, we found that SIPA1L2 directly binds to TrkB and application of a TAT-peptide encompassing the binding region for TrkB in SIPA1L2 induces a similar phenotype in vivo and in vitro in wild-type (wt) mice like those observed in sipa1l2 ko mice. We found that SIPA1L2 links the receptor tyrosine kinase to a dynein motor via a direct interaction with the adaptor protein Snapin which allows retrograde transport. Interestingly, SIPA1L2 concurrently interacts with Light chain 3 (LC3), a marker for autophagosomes that is involved in substrate selection, and this interaction promotes SIPA1L2 RapGAP activity. While autophagosomes are continuously generated at axon terminals, very little is known about the synaptic role of autophagy. Here we show that SIPA1L2 associates with amphisomes, organelles from the autophagic pathway that result from the fusion of autophagosomes with late endosomes, that are positive for the late endosome marker Rab7 as well as LC3 and TrkB. This configuration allows LC3 to tightly control TrkB signaling via interaction with SIPA1L2, which increases the RapGAP activity and promotes Rap1/ERK inactivation. We show that these amphisomes traffick retrogradely along axons, stop at presynaptic boutons and both motility and signaling are controlled by SIPA1L2’s RapGAP activity that reduces the velocity of amphisome transport. Presynaptic LTP induces a protein kinase A (PKA)-dependent dissociation of the SIPA1L2/Snapin complex from dynein intermediate chain (DIC). This increases dwelling time of the amphisome at presynaptic boutons and PKA phosphorylation of SIPA1L2 reduces RapGAP activity, therefore, enabling local TrkB signaling at boutons, which in turn promotes neurotransmitter release. Collectively, the data suggest that retrograde axonal transport of BDNF/TrkB occurs in neuronal amphisomes that allow local control of TrkB signaling and are involved in plasticity-relevant local signaling at presynaptic boutons.

Results

MF-LTP and pattern separation deficits in sipa1l2^−/− mice

To study the neuronal function of SIPA1L2, we generated sipa1l2 ko mice (Supplementary Fig. 2a–d). No major morphological abnormalities were observed in the cerebellum and DG (Supplementary Fig. 2e–k), motor learning or coordination (Supplementary Fig. 2l–m). The number of adult-born granule cells and measures of general DG excitability and postsynaptic function were all normal in sipa1l2^−/− mice (Supplementary Figs. 2n–o and 3). However, a significant deficit was found when we assessed MF LTP, a form of plasticity presynaptically expressed at MF boutons of DG granule cells (Fig. 1a, b). Accordingly, we also observed a strong impairment in spatial pattern separation, a cognitive process associated with proper DG function and MF LTP^{12,13,14,15,16}, that is responsible for the disambiguation and independent storage of similar memories (Fig. 1c–e). No changes were found in novel object location and recognition paradigms that are sensitive to perturbation of synaptic function in hippocampal CA1 neurons (Fig. 1f). Spatial pattern separation as well as MF LTP have been shown to depend on BDNF/TrkB signaling in the DG^11,13 and we indeed found a reduction in MF LTP when we chelated endogenous BDNF during perfusion of slices with TrkB-Fc bodies (Fig. 1g, h), resembling the decline in late phase LTP found in sipa1l2^−/− mice.

The cytoplasmic domain of TrkB directly binds to SIPA1L2

These experiments raised the question of whether SIPA1L2 might be involved in presynaptic BDNF-TrkB signaling. BDNF/TrkB are internalized at distal axons and transported retrogradely in a dynein-dependent manner as signaling endosomes^{7,17,4a). Coimmunoprecipitation experiments from extracts of rat hippocampi revealed that the protein is indeed present in immunoprecipitates generated with a TrkB-specific antibody and vice versa, TrkB could be precipitated with a SIPA1L2 antibody (Fig. 2c; Supplementary Fig. 4b). Moreover, full-length TrkB and SIPA1L2 are present in transport complexes immunoprecipitated by an antibody directed against DIC (Fig. 2d) and both proteins are localized to axon terminals in hippocampal primary neurons (Fig. 2e) where they were found in very close association as revealed by STED imaging (Fig. 2f, g).}

Next, we mapped the binding region in both proteins with Yeast-Two Hybrid (YTH) and found that the ActI-domain of SIPA1L2 interacts in the TrkB cytosolic domain with the first 12 juxtamembraneous amino acids (aa) (454–465) and the first 23 aa of the tyrosine kinase domain (537–559) (Supplementary Fig. 4c–d). Bacterially expressed GST- and MBP-fusion proteins revealed a direct interaction of cytoplasmic TrkB with the ActI but not the ActII domain of SIPA1L2 in pull-down assays (Supplementary Fig. 4e). We isolated 14 aa in SIPA1L2-ActI that are crucial for the association with TrkB (Fig. 2h; Supplementary Fig. 4f–h) and that happen to be unique to SIPA1L2 when compared to other SIPA1L family members (Supplementary Fig. 1c) and designed a TAT-peptide containing this region. This TAT-peptide competed for binding to TrkB in a pull-down assay (Fig. 2i) and when infused into the DG of wt animals (Fig. 2j; Supplementary Fig. 4i) induced a deficit in spatial pattern separation (Fig. 2k, l) similar to the one observed in sipa1l2^−/− mice (Fig. 1e). In addition, the bath perfusion of the TAT-peptide also reduced the amplitude of MF-LTP in acute slices (Fig. 2m). Thus, interruption of the SIPA1L2-TrkB interaction in wt mice mimics the deficits of sipa1l2^−/− mice.

SIPA1L2 interacts with Snapin and enables TrkB trafficking

Dynein-dependent retrograde trafficking of TrkB-signaling endosomes in axons requires Snapin, an adaptor protein that recruits dynein by interacting with DIC

cLTP prolongs the stopovers of TrkB-amphisomes at boutons

Presynaptic LTP results in activation of PKA at MF boutons and enhanced synaptic function that is largely mediated by PKA-dependent phosphorylation of different components of the presynaptic release machinery^{26,27,28,29,30}. PKA-mediated phosphorylation of Snapin at Ser50 is crucial for its dissociation from the DIC and a phosphomimetic Snapin-S50D mutant is largely immobile³¹. Heterologous coimmunoprecipitation experiments revealed that both phosphomimetic (S50D) as well as phospho-deficient (S50A) Snapin interact with tRFP tagged SIPA1L2-470-1025 (Fig. 6a). However, in contrast to the phospho-deficient protein, DIC did not coimmunoprecipitate with phosphomimetic Snapin (Fig. 6a), indicating that PKA-dependent phosphorylation induces the dissociation of the protein from DIC. Consistent with previous work³¹, time-lapse imaging revealed that phosphomimetic Snapin is largely stationary and was often found in proximity to immobile fl-SIPA1L2-mCherry at axon terminals (Fig. 6b, c). We therefore next wondered whether changes in synaptic activity could affect amphisome trafficking among presynaptic terminals. Trafficking of SIPA1L2/LC3b among presynaptic terminals labeled by anti-Synaptotagmin 1^Oyster650 antibodies revealed an enhancement in dwelling time upon induction of chemical LTP (cLTP) (Fig. 6d–f). The PKA inhibitor H89 prevented this effect (Fig. 6e, f).

It has been reported that PKA-dependent phosphorylation of Ser499 of Rap1GAP negatively regulates RapGAP activity³². Sequence analysis of SIPA1L2 revealed a high scoring PKA phosphorylation motif (S990-RxxpS motif/ 0,757/ Supplementary Fig. 1d) that is located close to the region that corresponds to the regulatory PKA sites (pSer 499) of Rap1GAP and that is well preserved between SIPA1L family members (Supplementary Fig. 1c). When we tested the hypothesis that PKA in analogy to Rap1GAP might negatively regulate RapGAP activity of SIPA1L2, we found that phosphomimetic SIPA1L2-470-1025-S990D indeed hydrolyzed very little recombinant Rap1b-GTP (Fig. 6g–i), indicating a negative regulation of RapGAP activity by PKA. Collectively these data suggest that following induction of presynaptic plasticity, PKA phosphorylation of Snapin induces the dissociation of the amphisome from Dynein and enhances its residing time at presynaptic boutons. Concomitant phosphorylation of SIPA1L2 diminishes its RapGAP activity and thereby potentially facilitates ERK signaling.

Amphisomes activate ERK at boutons and enhance release

Amphisomes are believed to be transient intermediate organelles that in nonneuronal cells rapidly enter a degradative lysosomal pathway. However, lysosomes are not abundant if at all present in distal axons³³. In primary cultures, STED imaging revealed that the lysosomal marker LAMP1 was indeed mostly absent from presynaptic boutons, rendering unlikely that SIPA1L2-TrkB-LC3b associates with a degradative organelle at axon terminals (Supplementary Fig. 6a). A recent study showed that TrkB is transported on autophagosomes to the soma where it regulates gene expression³⁴. This transport requires association of the Clathrin adaptor AP2 with LC3 and DIC³⁴. Therefore, we speculated that amphisomes could constitute more persistent entities in axons where they could serve signaling functions at presynaptic boutons. Accordingly, we found that SIPA1L2/LC3/TrkB complexes are positive for the late endosome marker Rab7 (Fig. 7a; Supplementary Fig. 6b–c). Moreover, kinase-active pTrkB^Y515 showed a significant degree of colocalization with SIPA1L2 at presynaptic boutons in line with the high association of LC3, SIPA1L2 and TrkB observed before (Fig. 7b, c). This is expected to be higher when assessing colocalization in only those boutons in which SIPA1L2 is present. Accordingly, intensities of pTrkB^Y515 are higher in SIPA1L2-positive boutons (Fig. 7d–f). This tight association of SIPA1L2 with pTrkB^Y515 as well as TrkB with LC3 (Fig. 7g, h) and Rap1 with SIPA1L2 and LC3 (Supplementary Fig. 6d) at presynapses indicate that SIPA1L2 might be part of a signaling TrkB amphisome. We also found an extensive colocalization with AP2 in axons and synaptic boutons (Supplementary Fig. 6e–g), suggesting that SIPA1L2-TrkB amphisomes are long-range organelles that will traffic retrogradely to reach the soma³⁴. Moreover, TrkB and SIPA1L2 associate with an autophagosome enriched fraction from rat brain lysates that is also positive for LC3, Rab7 and Snapin (Fig. 7i, j). Of note, SIPA1L2 was not found in the lysosome fraction (Fig. 7i, j).

What could be the consequence of amphisome stopover at axon terminals? Local ERK activation has been linked to enhanced neurotransmitter release and presynaptic plasticity^35,36. We took advantage of a FRET-based ERK sensor³⁷ that we targeted to the presynaptic compartment following fusion to Synaptophysin-1 (Fig. 8a–e; Supplementary Fig. 6h). We then imaged changes in the GFP fluorescence lifetime, the donor of the FRET pair, coinciding with the stopover of amphisomes, which we identified using either tagged versions of SIPA1L2 or LC3b. Synaptic visits were determined by kymograph analysis (Fig. 8a). Quantification of ∆Lifetime_GFP upon the arrival of the amphisome at the bouton (time 0) indeed correlated with a significant decrease in the GFP lifetime (Fig. 8b–e), indicating a higher FRET efficiency due to ERK activation and subsequent phosphorylation of the sensor³⁷. Importantly, this decrease was not observed when the amphisome passed by a bouton without stop** (nonvisited terminals) or in axons where no amphisomal trafficking was observed during the recording time (Fig. 8c–e).

ERK activation in presynaptic plasticity results in phosphorylation of Synapsin I (Syn-I), a protein known to play a role in BDNF-mediated increase of neurotransmitter release³⁶. Ultrastructural analysis revealed no major alterations in presynaptic bouton organization and vesicle content in sipa1l2^−/− as compared to wt mice (Supplementary Fig. 6i–j). Quantification of ERK-dependent phosphorylation of Syn-I in hippocampal primary neurons from wt mice revealed a significant enhancement of the pSyn/Syn ratio at boutons following BDNF application (Fig. 8f, g) without changes in their number (Supplementary Fig. 6k). The BDNF-induced enhancement of the pSyn/Syn ratio was absent in sipa1l2^−/− neurons and could be rescued by re-expression of fl-SIPA1L2 in ko neurons. However, no rescue was observed when we re-expressed the SIPA1L2-Δ14 mutant lacking the TrkB binding region (Fig. 8f, g).

To directly assess the effects of the activation of these molecular pathways in transmitter release, we combined unloading of FM 4-64 dyes to monitor synaptic vesicle fusion with live imaging of LC3b/TrkB trafficking and compared unloading rates at visited and nonvisited boutons (Fig. 8h–j). Analysis of the unloading rates before and after a stopover revealed a significant enhancement in the majority of the analyzed boutons visited by both TrkB-GFP (Fig. 8k) and GFP-LC3b (Fig. 8l). Importantly, this increase was not observed in nonvisited neighboring boutons (Fig. 8m).

Discussion

Autophagosomes in neurons are formed continuously in distal axon terminals^38,39 from where the autophagic flux of cargoes derived from synapses is directed towards the soma in a dynein-dependent manner^{19,38,39,40,41,42}. Classical stimuli like starvation have relatively little effect on neuronal autophagic flux⁴⁰. However, it was shown previously that synaptic activity transiently upregulates autophagy at presynaptic terminals^41,43,44,45 and autophagosome formation might be therefore regulated locally in individual boutons. Although there is growing appreciation that neuronal autophagy might serve synaptic function^46,47,48, it has not been shown yet that it might be a mechanism used to mediate activity-dependent synaptic change. In the present work, we introduce a signaling mechanism that is based on the incorporation of TrkB receptors from late endosomes to autophagosomes. This fusion has been largely associated with the termination of signaling⁴⁹ and evidence for the existence of a signaling amphisome was scarce⁵⁰. We found that the resulting organelle has indeed features of an amphisome with signaling properties whose trafficking and signaling capabilities are tightly controlled by SIPA1L2 and its association with TrkB, Snapin, and LC3. Intriguingly, binding of LC3 enhances RapGAP activity and thereby negatively interferes with TrkB-induced Rap signaling, which is upstream of ERK activation (Fig. 9). PKA phosphorylation of Snapin and SIPA1L2 at presynaptic sites immobilizes the amphisome at axon terminals, terminates RapGAP activity and thereby allows TrkB/Rap1 signaling that will facilitate transmitter release (Fig. 9). It was proposed previously that autophagosomes contain active TrkB complexes³⁴. In this study, we provide evidence that TrkB signaling endosomes are in fact amphisomes whose formation is likely linked to the regulation of neuronal autophagy. We propose that neuronal amphisomes are stable prelysosomal hybrid organelles with signaling capabilities based on the findings that (i) they were immunopositive for the late endosome marker Rab7 but not the lysosomal marker LAMP1, (ii) TrkB in presynaptic boutons is invariably found in close proximity to SIPA1L2 and phosphorylated at Y515, which is a crucial phosphosite for ERK signaling, (iii) amphisome stopover occurred predominantly at active release sites, (iv) amphisome trafficking to boutons correlated with enhanced levels of phosphorylated Syn-I and ERK activation and (v) finally, enhanced transmitter release.

What could be the advantage of assembling a stable hybrid organelle for signaling at axonal boutons? Axon terminals and distal axons in mature neurons contain very few lysosomes and acidification and proteolytical cleavage are significantly delayed until amphisomes reach the soma^33,39. Most important, whereas the lumen of autophagosomes becomes more acidified during retrograde transport and lysosomal markers accumulate, levels of cathepsins, the major lysosomal proteases, remain very low compared to those in the soma⁵¹. If one takes into account the extreme distances in axonal transport, amphisomes will constitute stable entities long before acquiring a lysosomal identity near the neuronal cell body. Since autophagosomes fuse with late endosomes to undergo robust retrograde transport¹⁹, it is probably inevitable that, in the absence of autolysosome formation, amphisomes serve as signaling and sorting platforms while trafficking in a retrograde direction. This makes endosomal sorting processes at axon terminals dispensable and would give an answer to the long-standing question of why neurons transport autophagic and endocytic cargos back to the cell body for degradation instead of disposing of them locally.

It is likely that amphisomes collect additional cargo during visits at synapses that may differently determine their properties and vesicular fate while propagating retrogradely to the soma. Sustained signaling capacity and diversity will promote and integrate local synaptic function with long-range signaling while evading lysosomal degradation. Specificity for certain synaptic connections is conceivable and in the case of SIPA1L2 might come from docking to presynaptic sites via for instance PDZ domain-dependent interactions. The idea of selective amphisome signaling at subpopulations of boutons is appealing since TrkB/BDNF facilitation of presynaptic transmitter release possibly requires more sophisticated signaling than the one provided by the classical TrkB signaling endosomes that lack local signaling function and selectivity for individual boutons required to induce clustered presynaptic plasticity like it was proposed by Staras et al.⁵². This study demonstrated the existence of a vesicle pool that is not confined to a synapse but spans multiple terminals. Vesicles within this superpool are highly mobile and are rapidly exchanged between terminals. Focal BDNF application suggests the involvement of a local TrkB-receptor-dependent mechanism for synapse-specific regulation of presynaptic vesicle pools through control of vesicle release and capture to or from the extrasynaptic pool⁵². Thus, clustered presynaptic plasticity is another conceivable function of amphisome signaling. This is also interesting in light of several studies that have shown that MF LTP is expressed presynaptically and requires cAMP–PKA signaling⁵³. Our results suggest that the SIPA1L2 amphisome is a likely substrate for PKA-dependent LTP. Along these lines, we speculate that at MF synapses SIPA1L2-related amphisome signaling is important for the maintenance of LTP, which in turn requires BDNF/TrkB signaling and is important for pattern separation.

Several adaptors have been described for antero- and retrograde transport of TrkB^{18,34,54,55,56,57}. Many of these adaptors show also an association with autophagosomes^58,59,60 and synergies likely exist between adaptors. Interestingly, Snapin is reportedly also an adaptor for axonal lysosomes⁵⁹, which further supports the idea of a single transport complex slowly acquiring a lysosomal identity during retrograde movement. We could show that SIPA1L2-mediated ride-on service for TrkB-containing amphisomes enables local and long-distance signaling (Fig. 9). In this context, it should be mentioned that other SIPA1L family members exhibit a similar domain organization with a high degree of similarity in the RapGAP, PDZ and CC-leucine zipper domain (Supplementary Fig. 1). It is thus very likely that all of them will bind Snapin and LC3 while they might differ in cargo selection for retrograde transport of amphisomes but also additional functions of the SIPA1L family in autophagy are conceivable.

Methods

Animals

Animals were maintained in the animal facility of the Leibniz Institute for Neurobiology, Magdeburg (Germany) or ZMNH, Hamburg (Germany) under controlled environmental conditions.

All animal experiments have complied with all ethical regulations for animal testing and research in accordance with the European Communities Council Directive (2010/63/EU) and were approved by the ethics committees of Sachsen-Anhalt/Germany (reference number 42502-2-1264 LIN and 42502-2-1284 UNIMD) or of the city-state of Hamburg (Behörde für Gesundheit und Verbraucherschutz, Fachbereich Veterinärwesen) and the animal care committee of the University Medical Center Hamburg-Eppendorf.

Generation of sipa1l2 ^−/− mice

For a constitutive knockout generation, exon 2 of the SPAR2/sipa1l2 locus (Gene ID: 244668; RefSeqs NM_001081337.1 (mRNA), NP_001074806.1 (protein)) was replaced by a IRESLacZ reporter gene and a neomycin phosphoribosyltransferase selection cassette by homologous recombination. The targeting vector was constructed by amplifying homologous regions from 129S6/Sv/Ev mouse genomic DNA using primers described in Supplementary Table 1.

Gene targeting was performed using ES cells (CCB; 129S6/Sv/Ev strain) and chimeras were generated by injection into C57/Bl6 blastocysts. All experiments described here were performed with mice backcrossed for at least ten generations to C57BL/6J background. Genoty** was performed with primers described in Supplementary Table 1 resulting in bands of 200 bp for wt and 400 bp for ko condition.

Antibodies

A list of antibodies used in this study and corresponding dilutions is available in Supplementary Table 1.

Cloning

Sources of the main constructs and primers used in this study are available in Supplementary Tables 1 and 2. For Snapin knockdown experiments, a published Snapin KD sequence⁶¹ was cloned into the pSIH-H1 shRNA vector (System Biosciences). Inserted sequences are described in Supplementary Table 1.

The Sy-EKAR vector was created by PCR amplification of EKAR-GFP/RFP (ref. ³⁷; Addgene #18680) to which a 4Gly linker domain (GGTGGCGGTGGA) was incorporated in the N-terminus (see Supplementary Table 1). This was subcloned into the CMV: ratSyGCaMP2 (Addgene #26124), from where GCaMP2 was removed. SIPA1L2 mutants were generated using the Q5 site-directed mutagenesis kit (NEB).

Yeast Two-Hybrid

The Yeast-Two-Hybrid assay was performed as described in ref. ⁶².

Electrophysiology in acute hippocampal slices

MF-LTP induction was performed as described in ref. ¹¹. Hippocampal slices from 11–16-week-old C57BL/6J or sipa1l2^−/− mice were cut and preincubated for 3 h prior recordings. When required, slices were preincubated for 3 h before recording in carbogenated ACSF either with 1 µM of TAT-SIPA1L2 peptide or with 1 µM of TAT-scrambled peptide as a control, as well as with or without 5 µg/ml TrkB-Fc.

For assessment of postsynaptic DG LTP, field-EPSPs were measured with an electrode positioned at the middle of the molecular layer of the DG. The medial perforant path was stimulated with biphasic constant current pulses (0.1 ms per half-wave duration). Different theta-burst stimulation protocols (TBS) or a high- frequency stimulation (HFS) protocol were used for induction of synaptic plasticity. The TBS protocols consisted of either one episode of brief presynaptic bursts (ten pulses of 0.2 ms per half-wave duration delivered @10 Hz) repeated ten times @5 Hz, two of these episodes (inter-episode interval 10 s) or four episodes (inter-episode interval 10 s) repeated four times every 5 min. The HFS protocol consisted of four trains of 100 pulses (0.2 ms per half-wave duration) @100 Hz, inter-train interval 5 min.

Behavioral tests

The behavioral spatial pattern separation task was performed as described¹³ with minor modifications. For object location and novel object recognition task, two equal objects were placed at the equidistant position from the walls of the arena, and the animals were free to explore them. Twenty-four hours later, novel location recognition was assessed as one of the same familiarized objects was displaced to a new location and mice were again left free to explore both objects over 20 min. On the third day, a novel object recognition test took place as one of the familiarized objects was now exchanged for a novel object. Animals were again left for 20 min to explore the objects before being returned to the home-cage. In experiments involving rotarod, animals were tested on an accelerating rotarod apparatus two trials per day, for 5 consecutive days, in which the speed of the rod was gradually increased each day (from 15 to 36 rpm). On day 5, each animal was submitted to four trials lasting 300 s each, during which the rotation speed gradually increased from 4 to 40 rpm. The latency to fall was recorded.

In experiments requiring infusion of TAT-peptides in DG, animals (wt) underwent sample phase training of the spatial pattern separation protocol, and 5 min after the session received an intra-DG infusion of either 1 mM TAT-SIPA1L2 peptide or TAT-scrambled conjugated with fluorescin. Anesthesia was induced at 5% isofluorane in O₂/N₂O mixture (Rothacher Medical GmbH., Switzerland) and mice were placed in the stereotaxic frame (World Precision Instruments). During surgery, anesthesia was maintained at 1.5–2.0%. Craniotomy was done targeting the dorsal dentate gyrus with stereotaxic coordinates, anterioposterior (AP) −2.0 mm, mediolateral (ML) ±1.4 mm from Bregma and dorsoventral (DV) −1.6 mm (from brain surface). Each mouse received 1.5 μl bilateral infusion of a respective peptide at a flow rate of 0.5 μl/min. The effect of TAT-peptide infusion was assessed 24 h later in the choice phase of pattern separation task. After the completion of the task, mice were deeply anesthetized and perfused transcardially with PBS and 4% paraformaldehyde (PFA) in PBS solution to verify DG localization of injections.

Cell culture, transfections and immunostaining

Maintenance and transfection of MRC5, HEK293T and COS-7 cells were performed as described in ref. ⁶³. HEK293T were obtained from Leibniz Institute DSMZ (Cat#ACC635; RRID:CVCL_0063), COS7 were obtained from ATCC (Cat#CRL-1651; RRID:CVCL_0224) and MRC-5 cells were obtained from KCLB (Cat#10171; RRID:CVCL_0440).

Primary rat (Sprague Dawley, E18) hippocampal cultures were prepared as previously described⁶⁴. Mouse hippocampi were dissected and prepared as described in ref. ⁶⁴ and neurons were plated at a density of 35,000 cells/18 mm-coverslip and maintained as described. For live imaging experiments, primary hippocampal cultures were transfected at 10–14 DIV using Lipofectamine 2000 (Thermo Fisher Scientific) according to the manufacturer’s instructions and imaged 48 h after transfection. For knockdown experiments, neurons were transfected at 8 DIV and imaged 96 h after transfection.

Immunocytochemistry was performed as described in ref. ⁶⁴. Sequential labeling steps were included to avoid cross-labeling. For detection of SIPA1L2, a heat-based antigen retrieval protocol was used. This included the immersion of the cells into a 10 μM sodium citrate solution (pH = 9) for 30 min at 80 °C before proceeding with the standard protocol. The pH of the sodium citrate (Fluka) solution was adjusted with 5 M NaOH.

For immunostaining of cryosections, brains of adult sipa1l2 wt and ko mice were post-fixed in 4% PFA and 0.5 M sucrose for 24 h and 40-μm-thick cryosections were cut.

For Nissl staining, sections were acidified with 0.05 M acetate buffer (pH 4) for 5 min and incubated with 0.05% cresyl violet acetate solution for 10 min. Sections were further incubated with 0.05 M acetate buffer (pH 4) for 3 min and dehydrated by ethanol steps. For the volumetric analysis of the DG, serial sections were collected throughout the region. Timm staining was performed as described⁶⁵.

EM analysis

For ultrastructural analysis, ultrathin sections were prepared from six adult male animals as described in ref. ⁶⁶. Quantification was performed as described in ref. ⁶⁶.

Imaging of primary cultures and kymograph preparation

Rat primary neurons overexpressing fl-SIPA1L2-mCherry together with TrkB-GFP, GFP-LC3b or GFP-Snapin were placed in a Ludin chamber (Life Imaging Services) and imaging was performed 24–48 h later at 37 °C 5%CO₂ on a VisiScope TIRF/FRAP imaging system from Visitron Systems based on Nikon Ti-E. The system is equipped with the Perfect Focus System (Nikon), Nikon CFI Apo TIRF 100×, 1.49N.A. oil objective, a back focal TIRF scanner for suppression of interference fringes (iLas2, Roper Scientific), and controlled with VisiView software (Visitron Systems). 488 and 561 nm laser lanes were used to excite the respective fluorophores whose fluorescence was collected through ET 488/561 Laser Quad Band filters to a The ORCA-Flash 4.0 LT sCMOS camera. Time-lapse imaging of neuronal axons was recorded at 0.5 Hz for 4 min in neuronal conditioned media. For testing the coclustering of SIPA1L2 with TrkB, SIPA1L2-mCherry or SIPA1L2-∆14-mCherry were coexpressed with TrkB-GFP in MRC5 cells and cells were imaged 24 h later in growing media for 1 min at 2 Hz. For recruitment assay of RapGAP-SIPA1L2 and Snapin, COS7 cells were cotransfected with RapGAP-PDZ-SIPA1L2-tRFP or a tRFP empty vector as control and GFP-Snapin. Coverslips were mounted on an imaging ring 24 h after transfections and cells were imaged in DMEM-based growing media. For cotrafficking of SIPA1L2 and Snapin, fl-SIPA1L2-mCherry and GFP-Snapin were overexpressed in COS7 cells for 24 h and imaged in growing media for 2.5 min at 1 Hz. Imaging of MRC5 and COS7 cells was done in the same VisiScope imaging system described above.

The analysis was performed using the free software Fiji from ImageJ (http://imagej.nih.gov/ij/) and kymographs were created using KymographClear⁶⁷ by drawing a line along axons and Fourier-filtered kymographs generated by the plugging were displayed in figures. Retrograde and anterograde trajectories were identified by the respective location of the cell soma or axonal tip during imaging. Stationary trajectories were considered as those moving <3 μm. Axons from three independent experiments were analyzed.

In experiments where Snapin was knocked-down, imaging was performed 96 h after transfection on a Leica TCS SP5 system controlled by Leica LAS AF software using HCX PL APO 63×1.40. Areas of ~60 × 15 µM (512 × 128 pixels) were scanned using 488, 561 and 633 nm laser lanes, at 37 °C and 5%CO₂. SNAP-Cell 647 SiR (0.5 µM) was added 1 h before starting imaging in order to visualize TrkB-SNAP.

Imaged axons were selected according to the following morphological criteria: (i) long thin processes growing far from the somatic region with constant width; (ii) without recurrent bifurcations and (iii) lacking protrusions or spines.

Live imaging of FM₄₋₆₄ unloading

Primary hippocampal cultures (14–16 DIV) overexpressing TrkB-GFP or GFP-LC3b were placed into a field stimulation chamber (Warner Instruments) 24–48h after transfection. The field stimulation chamber contains two platinum wire electrodes placed 10 mm apart. Cells were incubated with 10 µM FM_4–64 (Thermo Fisher) in extracellular imaging buffer (EIB: 119 mM NaCl; 2.5 mM KCl; 2 mM CaCl₂; 2 mM MgCl₂; 30 mM Glucose; 25 mM HEPES in H₂O at pH 7.4) containing 10 µM AP-5 and 4 µM CNQX⁶⁸. Dye loading was achieved by delivering pulses for 30 s@20 Hz. Unbound dye was washed-out with EIB containing 1 mM ADVASEP-7 (Sigma-Aldrich) for 1 min followed by two washes with EIB at 37 °C. After 10 min, dye unloading was triggered by a train of 900 pulses @10 Hz in the presence of AP-5 (50 µM) and CNQX (10 µM) and dye unloading was imaged at a frequency of 1 Hz for 110 s⁶⁸. Ten frames were acquired as a baseline before stimulation. Trafficking of either TrkB-GFP or GFP-LC3b was recorded immediately after the stimulation for an additional 10 min @1 Hz. After that, a second dye unloading protocol was delivered and imaged as described.

Imaging was performed at 37 °C 5%CO₂ in the VisiScope TIRF/FRAP imaging system from Visitron Systems based on Nikon Ti-E described above. Laser lines of 488 and 633 nm were used to activate the respective fluorophores whose fluorescence was collected ET 488/640 Laser Quad Band filters to The ORCA-Flash 4.0 LT sCMOS camera. Pulses were evoked by delivering currents of 60 mA for 1 ms using an isolator unit controlled by a pulse generator (Master-8). Boutons were considered as visited when trajectories overlapped with the signal from the FM dye for >3 vertical pixels in the kymograph while the term nonvisited was used for those boutons within the same axon where the overlap** signals were lasting <3 pixels. Circular ROIs were placed in both types of boutons using Times Series Analyzer V3 from Fiji and mean gray values per ROI per frame were calculated from both streams of images acquired during unloading protocols. Data from each ROI were fitted to a one-phase decay from which tau was calculated using GraphPad Prism.

Amphisomal trafficking to presynaptic boutons

Primary hippocampal neurons 14−16 DIV overexpressing tRFP-LC3b and either fl-SIPA1L2-GFP or SIPA1L2-N705A-GFP were incubated in growing media in the presence of an antibody anti-Synaptotagmin1 coupled to Oyster 660 (1:250) for 1–2 h at 37 °C 5%CO₂. Imaging was performed for 5 min at 1 Hz at 37 °C 5% CO₂ on a Leica TCS SP5 system controlled by Leica LAS AF software using HCX PL APO 63×1.40. Areas of ~60 × 15 µM (512 × 128 pixels) were scanned using 488, 561 and 633 nm laser lanes. Fluorescence was collected using three HyD detectors.

For experiments cLTP induction was required, neurons in growing media were treated with Rolipram (0.1 µM) and Forskolin (50 µM) for 10 min and cells were imaged after media replacement with EIB . When needed, H89 (10 µM) was added for 30 min before proceeding with the cLTP induction.

Dwelling time of the amphisome in boutons was calculated from kymographs by drawing a vertical line using Fiji in cotrajectories that overlapped with the synaptic marker and the number of pixels was quantified. Only those stopovers where the overlap occurs for >3 vertical pixels were considered. Instant velocity was calculated from the angle of each cotrajectory obtained by drawing a line along each trajectory. Run-length was also assessed by drawing a line and measuring its dimensions using Fiji. Frequency distributions were generated in GraphPad Prism.

Imaging and quantification of pSyn/Syn ratio

Hippocampal primary cultures from wt and sipa1l2^−/− mice were transfected at 10 DIV with either fl-SIPA1L2-mCherry, SIPA1L2-∆14-mCherry or empty vector for control. Transfected neurons were treated at 14 DIV with TrkB-Fc (500 ng) overnight or with BDNF (100 ng) for 30 min.

Immunocytochemistry was performed with anti-Synapsin I,II, anti-phospho Synapsin (Ser62) and anti-mCherry primary antibodies. Stack images were acquired in a Leica TCS SP5 system controlled by Leica LAS AF software using HCX PL APO 63 × 1.40. Areas of 82 × 82 µM were scanned with 488, 568 and 635 nm laser lanes (12-bits, 80 × 80 nm pixel size, 700 Hz, Z-step 0.25 µm). Fluorescence was collected using three HyD detectors.

Circular regions of interest (ROIs) were placed using Time Series Analyzer v3 on the Synapsin signal and pSyn/Syn ratio was calculated for single boutons from the mean gray intensity obtained in Fiji for each ROI. Boutons from the same axon were averaged and average from axons is plotted on the graph. For each rescue group, data were normalized to the group of neurons treated with TrkB-Fc.

Imaging of Sy-EKAR

Neurons overexpressing Sy-EKAR and either with SNAP-SIPA1L2 or SNAP-LC3b were imaged in EIB. The far-red SNAP-Cell 647 SiR substrate at a concentration of 0.5 µM was added to the growing media for 1 h and was washed out (3×) with EIB.

FLIM measurements were carried out at 37 °C and 5%CO₂ using an Abberior multichannel confocal/STED microscope based on a Nikon Ti-E microscope body with perfect focus system. For excitation three pulsed lasers at i.e. 470, 561, 640 nm were employed. A ×60 P-Apo oil immersion objective (NA 1.4) was used. In the detection path, the emitted light was directed through a pinhole set to match 1 Airy at 540 nm. Three avalanche photodiodes APDs were used for recording the fluorescence signal in the green 470−520 nm, red 595−635 nm and far-red 615–755 nm range. For measuring the fluorescence lifetime a Becker&Hickl SPC150 TCSPC board was used after setting the APDs in single-photon count mode (spcm).

A small area of interest containing 2–5 boutons was chosen. Two hundred FLIM images were recorded in streaming mode detecting only the green and the far-red channel. In this way, it was possible to record 2–4 FLIM images/s (pixel size was set to be 110 nm). The acquisition speed of any FLIM time-lapse experiment carried out in this work can be calculated using:

$${{V}} = 16{{vN}}_{{\mathrm{p}x}}{{N}}_{{\mathrm{p}y}},$$

(1)

where V is the acquisition speed in frames/s, 16 is the total number of line accumulations used for imaging the green and far-red channel, v is the pixel dwell time set to be 3 µs in all the experiments and ${{N}}_{{\mathrm{p}x}}/{{y}}$ is the x and y axis image pixel size. FLIM measurements were carried out in sampling the acquisition period of 19 ns in 50 steps of 0.38 ns.

FLIM images were analyzed by a self-made Matlab script where boutons were automatically localized by finding the local peaks of the intensity signal in the sum projection images and 5 × 5 pixels-ROIs were placed. The time series of the mean fluorescence lifetime was produced from each ROI by averaging the pixel by pixel measured lifetime. The lifetime information was extracted with the help of a least square algorithm. A mono-exponential decay function was used as a model function in the least square algorithm.

Boutons in axons without any detected trafficking were used as a control. Mean lifetime calculated from each bouton before - 4 frames -,during and after the visit of the amphisome and ∆Lifetime was calculated for each bouton. Lifetime₀ was considered as the mean lifetime in the frame before the arrival of the amphisome.

Confocal and STED imaging

Gated STED images were acquired with a Leica TCS SP8 STED 3X equipped with pulsed White Light Laser (WLL) and diode 405 nm laser for excitation and pulsed depletion with a 775 nm laser. A Leica HC APO CS2 ×100/1.40 oil objective was used. Images were taken as a single plane of 1024 × 1024 pixels and optical zoom ranging from 5 to 6, at 400 lines per seconds and 4× line averaging. Pixel size was 20–23 nm in xy. Time gates were set to 0.5 to 6 ns. Raw STED and confocal images were deconvoluted using Deconvolution wizard (Huygens Professional, SVI) and a theoretical point spread function (PSF) based on optical microscopic parameters until it reached a Quality threshold of 0.05.

Mander’s coefficient was calculated on deconvoluted images using either colocalization analyzer plug-in (Imaris, Bitplane) or the Colocalization test plugin from ImageJ. Briefly, the region of interests (ROIs) for presynaptic boutons were defined by intensity threshold of Bassoon staining. Manders M1 coefficient (SIPA1L2/ pTrkB^Y515) with Costes thresholding was measured within the synaptic boutons as a fraction of the total fluorescence found in common presynaptic ROIs.

Coimmunoprecipitation

Coimmunoprecipitation experiments were performed as described before⁶².

Briefly, ~500 mg of rat hippocampal tissue was homogenized in 2.5 ml/g in the following buffer: 10 mM HEPES, 320 mM sucrose, 1 mM EDTA, pH 7.4, PhosSTOP phosphatase inhibitors and complete proteases inhibitors (Roche Holding AG, Basel, Switzerland) by applying eight strokes at 900 rpm in a Teflon pistol homogenizer and subsequently centrifuging the samples for 10 min @800 × g. This procedure was repeated three times and the resulting supernatants were collected and centrifuged for 10 min at 3500 × g. The pellet was resuspended in 2.7 ml/g lysis buffer (150 mM NaCl, 50 mM TRIS, 0.5% DOC, 1% Triton X-100, PhosSTOP phosphatase inhibitors and complete proteases inhibitors) and incubated @4 °C with rotation. Samples were then centrifuged for 15 min @20,000 × g, and the supernatant was used for immunoprecipitation. An amount of 700 μl of tissue extract was incubated with 4 μl of goat anti-TrkB (R&D, 200 μg/μl) and 2 μl of goat IgG (sc-2028, 500 μg/μl), as well as 5 μl of rabbit anti-SIPA1L2 and 5 μl of rabbit IgG (sc-2027, 400 μg/μl) overnight @4 °C with rotation. Forty microliters of magnetic Protein G Dynabeads was added into the mixture for an additional 3 h for IgG coupling. The unbound fraction was removed and beads were washed with the extraction buffer. Proteins were eluted and subjected to SDS-PAGE.

For immunoprecipitation with DIC complex, 5 µg of anti-DIC antibodies or IgG mouse control antibodies were coupled to magnetic Protein G Dynabeads (Life Sci. Tech.) for 16 h. Subsequently, beads were washed three times in PBS pH 7.4 containing 0.1% (w/v) BSA and resuspended in 200 µl binding buffer (PBS, pH 7.4, containing 2 mM EDTA and 0.1% (w/v) BSA. For coimmunoprecipitation, 120 µl of the crude light membrane fraction was added and incubated for 16 h with constant rotating. All steps were performed at 4 °C. After substantial washing, proteins were eluted from the beads in 60 µl SDS sample buffer, boiled at 95 °C, subjected to 8% SDS-PAGE and analyzed by WB. Representative blots are shown uncropped and unprocessed in the Source Data file.

Lipid raft isolation

Cerebella were homogenized in lysis buffer (50 mM Hepes pH 7.4, 100 mM NaCl, 3% Brij 58, 5 mM EDTA, 10 mM Na₄P₂O₇, protease inhibitors). The lysate was mixed with 1.5 ml ice-cold sucrose (final concentration: 40%) and transferred to a Potter S (B.Braun, Germany). A homogenization step was performed and 3 ml of homogenate were transferred into a centrifuge tube. The sample was overlaid with 6 ml ice-cold 30% sucrose and then again overlaid with 3 ml ice-cold 5% sucrose and subjected to centrifugation for 20 h at 200,000 × g @4 °C. Twelve samples of 1 ml obtained and proteins of each sample were precipitated with acetone. Fraction 13 (pellet) was diluted in lysis buffer. Pellets from all fractions were lyophilized and resuspended in SDS protein sample buffer for SDS-PAGE and immunoblotting.

Heterologous coimmunoprecipitation

HEK-293T cells growing in T75 Flasks (Thermo Fisher Scientific) were transfected, harvested and lysed in 1 ml of RIPA buffer (50 mM Tris-HCl pH 7.4, 150 mM NaCl and 1% Triton X-100, 0.5% sodium deoxycholate and 0.1% sodium dodecyl sulfate (SDS)) for 1 h@4 °C. The lysate was cleared by centrifugation incubated with anti-GFP/myc-antibody-coated magnetic beads (MultiMACS GFP Isolation Kit #130-094-253 or Miltenyi Biotec GmbH, Germany). The coimmunoprecipitation was done following the manufacturer’s protocol (Mitenyibiotec, Bergisch-Gladbach, Germany).

For coimmunoprecipitation of fl-SIPA1L2-mCherry and SIPA1L2-Δ14-mCherry with TrkB-GFP, the following lysis buffer was used: 50 mM TRIS, 150 NaCl, 1% NP-40, 0.1% SDS, protease inhibitors (Complete, Roche), PhosphoStop (Roche). For heterologous coimmunoprecipitation of RapGAP and PDZ domains of SIPA1L2 with GFP-Snapin as well as coimmunoprecipitation of endogenous DIC and fl-SIPA1L2-mCherry with either GFP-Snapin-S50A or GFP-Snapin-S50D, the lysis buffer was without SDS. Representative blots are shown uncropped and unprocessed in the Source Data file.

Pull-down assays

Proteins were bacterially purified as previously described⁶².

For interaction assays between SIPA1L2 and TrkB, the matrix (amylose-MBP) alone or coupled with different fragments of the TrkB receptor was washed three times and resuspended in pull-down buffer (20 mM Tris pH 7.5, 1 mM DTT, 3 mM EDTA, 100 mM NaCl, 0.3% TritonX-100) and protease inhibitors (Complete, Roche). Subsequently, amylose resin was incubated with 2 μg of a GST-ActI-SIPA1L2-1-624 or GST-ActII-SIPA1L2-1026-1650 overnight @4 °C. Reciprocally, 10 µg of ether GST-SIPA1l2-86-99 or GST alone as a control was immobilized on Glutathione Sepharose resin equilibrated with PBS buffer (140 mM NaCl, 2.7 mM KCl, 10 mM Na₂HPO₄, 1.8 mM KH₂PO₄, pH 7.3, protease inhibitor (Complete, Roche)), incubated with 20 μg of purified MBP-TrkB-454-465 for 120 min@4 °C in PBS buffer and washed three times with PBST buffer (PBS, protease inhibitor, 0.1% Triton X-100). All samples were eluted with 2× SDS loading buffer and an equal amount of samples were used for SDS-PAGE.

For experiments with the TAT peptides, MBP-TrkB-454-821 was coupled to amylose resin (NE BioLabs) and incubated in the presence of 10× molar access of either TAT-SIPA1L2 or TAT-scr peptides with 1 μg of GST-SIPA1L2-1-624 fusion protein @4 °C overnight. The resin was washed three times with 1 ml of pull-down buffer (see buffer composition above) and boiled with 50 μl of 2× SDS sample buffer for 5 min before SDS-PAGE. Blots were then analyzed by immunodetection with anti-SIPA1L2 antibodies. For interaction between SIPA1L2 and LC3, 5 µg of Intein-RapGAP-SIPA1L2 (470–838) and Intein-Caldendrin were immobilized on chitin resin (NE BioLabs). The resin was washed with TBS buffer (150 mM NaCl, 20 mM Tris-Cl, pH 7.4, 5 mM MgCl₂, EDTA-free protease inhibitors (Complete, Roche)) and incubated with purified 6xHis-tag LC3b for 120 min @4 °C in TBS buffer either alone or in the presence of purified 6xHis-Snapin. Samples were then washed with TBS-T buffer (TBS + 0.1% Triton X-100), eluted with 2× SDS sample buffer, subjected to SDS-PAGE and immunoblotting. For pull-down assay of SIPA1L2 with endogenous LC3, whole brain extract was incubated with GST-SIPA1L2-RapGAP, GST-SIPA1L2-PDZ as well as with GST alone. Immunodetection was done with anti-LC3b antibodies. Representative blots are shown uncropped and unprocessed in the Source Data file.

Rap1-GAP activity assay

RapGAP activities were assessed using Active Rap1 detection kit (Cell Signaling)⁶⁹ following the manufacturer’s instructions. Briefly, the assay employs Rap1-binding domain (RBD) of RalGDS as an activation-specific probe for Rap1. Fused to GST-RalGDS-RBD allows to pull down the amount of nonhydrolized Rap1 in the presence of RapGAP. For experiments involving overexpression of fl-SIPA1L2, RapGAP and LIR-SIPA1L2 mutants GST-RalGDS pull down of GTP-bound Rap1 was performed from HEK293T cell extracts. For experiments involving LC3b and PKA phosphorylation of SIPA1L2-S990, Intein-tagged SIPA1L2-470-838, SIPA1L2-470-1025, SIPA1L2-470-1025-S990D, 6xHis-tagged Rap1b as well as 6xHis-targged LC3b were bacterially purified. Purified Rap1b was incubated with 50 mM GTP in 200 μl of loading buffer (50 mM TRIS, 100 mM NaCl, 2 mM EDTA, 1 mM DTT) for 20 min at 37 °C for loading recombinant Rap1b with GTP. The loading reaction was terminated by adding 10 mM MgCl₂. Subsequently, RapGAP assay was carried out in the presence of SIPA1L2-RapGAP domain (470–838 aa, ~20 μg), RapGAP-PDZ (470–1025, ~30 μg) and RapGAP-PDZ (470-1025-S990D, ~30 μg) of SIPA1L2 in the presence of either His-tagged LC3b (~50 μg) or His-SUMO (~50 μg) as a control for 15 min at 30 °C on shaker (650 rpm). Representative blots are shown uncropped and unprocessed in the Source Data file.

Preparation of autophagosomal-enriched fraction

A whole brain homogenate from two rats was subjected to density gradient separation and purification as already described with minor modifications⁷⁰. Briefly, rat brains were homogenized and subject to differential centrifugation through Nycodenz gradient (15, 20, 24, 26, 85%) for 3 h@105 × g. Each fraction (A1—15–20%, A2—20–24% and L—24–26% interfaces) was collected, diluted with Percoll and centrifuged for 1 h@105 × g to obtain a virtually pure pellet and analyzed using antibodies against SIPA1L2, TrkB, Snapin, LC3b, and Rab7.

Statistical analysis

Data in the manuscript are shown as mean ± SEM and n numbers used for statistics are depicted in each panel or figure legends from at least three independent experiments. Graphs and statistical analysis was made with GraphPad Prism (GraphPad Software). Statistical tests used are given in the figure legend of the corresponding experiment. Briefly, D’Agostino−Pearson omnibus normality tests were performed to assess the normality of samples. Subsequently, parametric and nonparametric tests were chosen accordingly. For comparison between normally distributed samples, two-tailed unpaired Student’s t test was used for comparing two groups and one-way ANOVA with Bonferroni’s posthoc test was used for comparison between more than two groups. For not normally distributed samples, Mann-Whitney U test or Kolmogorov-Smirnov test were used to compare two groups and Kruskal-Wallis with Dunn’s correction was used for comparing not normally distributed samples. P values < 0.05 were considered significant.

Reporting summary

Further information on research design is available in the Nature Research Reporting Summary linked to this article.

Data availability

All data supporting the findings described in this study are available from the corresponding author upon reasonable request. Source data from all representative blots shown in this study as well as data underlying all quantitative analysis performed in this study are provided with the manuscript as a Source Data file.

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Acknowledgements

The authors gratefully acknowledge the professional technical assistance of C. Borutzki, S. Hochmuth and M. Marunde, O. Kobler for help with imaging data processing, M. Lever for help with cloning. Supported by grants from the Deutsche Forschungsgemeinschaft (DFG) (KR 1879/9-1/FOR 2419, Kr1879/5-1/6-1/SFB 779 TPB8, BMBF ‘Energi’ FKZ: 01GQ1421B, The EU Joint Programme—Neurodegenerative Disease Research (JPND) project STAD and Leibniz Foundation to M.R.K.; DFG SFB 779 TPB8 to A.K.; DFG STO488/4-1/SFB779 TPB5 to O.S.; DFG 556/11-1 to M.K.; Leibniz SAW to E.D.G.; EU QLG3-CT-2001-01181 to M.C., E.D.G. G.M.G. was supported by the Alexander-von-Humboldt Foundation/CAPES post-doctoral research fellowship.

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Authors and Affiliations

Leibniz Group ‘Dendritic Organelles and Synaptic Function’, Center for Molecular Neurobiology, ZMNH, University Medical Center Hamburg-Eppendorf, 20251, Hamburg, Germany
Maria Andres-Alonso, Maximilian Borgmeyer, Silvia Diaz-Gonzalez & Michael R. Kreutz
RG Neuroplasticity, Leibniz Institute for Neurobiology, 39118, Magdeburg, Germany
Maria Andres-Alonso, Mohamed Raafet Ammar, Ioana Butnaru, Guilherme M. Gomes, Gustavo Acuña Sanhueza, Rajeev Raman, **An Yuanxiang, Jeffrey Lopez-Rojas, Tamar Macharadze, Christina Spilker, Anna Karpova & Michael R. Kreutz
Department of Genetics & Molecular Neurobiology, Institute of Biology, Otto-von-Guericke University, 39120, Magdeburg, Germany
Syed Ahsan Raza & Oliver Stork
Paradigm Therapeutics Ltd. Cambridge, UK Cerevance, Cambridge, UK
Nicola Brice & Mark Carlton
Institute of Molecular Neurogenetics, Center for Molecular Neurobiology, ZMNH, University Medical Center Hamburg-Eppendorf, 20251, Hamburg, Germany
Torben J. Hausrat & Matthias Kneussel
UKE Microscopy Imaging Facility, University Medical Center Hamburg-Eppendorf, 20246, Hamburg, Germany
Antonio Virgilio Failla
Center for Behavioral Brain Sciences, Otto von Guericke University, 39120, Magdeburg, Germany
Oliver Stork, Eckart D. Gundelfinger, Anna Karpova & Michael R. Kreutz
ZMNH Core Facility Morphology and Electron Microscopy, Center for Molecular Neurobiology, ZMNH, University Medical Center Hamburg-Eppendorf, 20251, Hamburg, Germany
Michaela Schweizer
Department of Neurochemistry and Molecular Biology, Leibniz Institute for Neurobiology, 39118, Magdeburg, Germany
Eckart D. Gundelfinger
Medical Faculty, Otto von Guericke University, 39120, Magdeburg, Germany
Eckart D. Gundelfinger

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Maria Andres-Alonso
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Mohamed Raafet Ammar
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Ioana Butnaru
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Guilherme M. Gomes
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Gustavo Acuña Sanhueza
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Rajeev Raman
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**An Yuanxiang
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Maximilian Borgmeyer
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Jeffrey Lopez-Rojas
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Syed Ahsan Raza
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Nicola Brice
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Torben J. Hausrat
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Tamar Macharadze
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Silvia Diaz-Gonzalez
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Mark Carlton
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Antonio Virgilio Failla
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Oliver Stork
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Michaela Schweizer
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Eckart D. Gundelfinger
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Matthias Kneussel
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Christina Spilker
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Anna Karpova
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Michael R. Kreutz
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Contributions

M.A.-A., C.S., A.K., M.R.K. designed the study, M.A.-A., A.K., M.R.A., I.B., G.A.S., G.M.G., R.R., P.Y., M.B., J.L.-R., T.J.H., T.M., C.S. conducted the experiments, M.A.-A., A.K., M.R.A., I.B., G.A.S., G.M.G., P.Y., M.B., J.L.-R., S.D.-G., M.S., T.M., C.S., M.R.K. analyzed the data, O.S., N.B., M.C., A.V.F., S.A.R., M.S., M.K., E.D.G., M.R.K., provided tools and reagents, M.A.-A., A.K. and M.R.K. wrote the paper and all authors commented and revised the manuscript.

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Correspondence to Anna Karpova or Michael R. Kreutz.

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Andres-Alonso, M., Ammar, M.R., Butnaru, I. et al. SIPA1L2 controls trafficking and local signaling of TrkB-containing amphisomes at presynaptic terminals. Nat Commun 10, 5448 (2019). https://doi.org/10.1038/s41467-019-13224-z

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Received: 01 March 2019
Accepted: 28 October 2019
Published: 29 November 2019
DOI: https://doi.org/10.1038/s41467-019-13224-z
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SIPA1L2 controls trafficking and local signaling of TrkB-containing amphisomes at presynaptic terminals

Abstract

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Introduction

Results

MF-LTP and pattern separation deficits in sipa1l2−/− mice

The cytoplasmic domain of TrkB directly binds to SIPA1L2

SIPA1L2 interacts with Snapin and enables TrkB trafficking

cLTP prolongs the stopovers of TrkB-amphisomes at boutons

Amphisomes activate ERK at boutons and enhance release

Discussion

Methods

Animals

Generation of sipa1l2 −/− mice

Antibodies

Cloning

Yeast Two-Hybrid

Electrophysiology in acute hippocampal slices

Behavioral tests

Cell culture, transfections and immunostaining

EM analysis

Imaging of primary cultures and kymograph preparation

Live imaging of FM4−64 unloading

Amphisomal trafficking to presynaptic boutons

Imaging and quantification of pSyn/Syn ratio

Imaging of Sy-EKAR

Confocal and STED imaging

Coimmunoprecipitation

Lipid raft isolation

Heterologous coimmunoprecipitation

Pull-down assays

Rap1-GAP activity assay

Preparation of autophagosomal-enriched fraction

Statistical analysis

Reporting summary

Data availability

References

Acknowledgements

Author information

Authors and Affiliations

Contributions

Corresponding authors

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Additional information

Supplementary information

Source data

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MF-LTP and pattern separation deficits in sipa1l2^−/− mice

Generation of sipa1l2 ^−/− mice

Live imaging of FM₄₋₆₄ unloading