Abstract
An ultrasensitive impedimetric immunosensor was developed to detect brain natriuretic peptide (BNP) for early diagnosis of heart failure. To construct this immunosensor, anti-BNP antibodies were immobilized covalently onto nanocomposite of chitosan–Au nanoparticles and reduced graphene oxide nanosheets (CHIT-Au@rGONs) electrodeposited onto pencil graphite electrode. This approach impedes charge transfer resistance (Rct) value proportionally to the BNP captured by antigen–antibody interactions. The observed Rct values by this immunosensor, were correlated with linear concentrations of BNP in the range, 1 × 10–2 to 1 × 103 pg/mL, with a limit of detection of 12 pg/mL and limit of quantification of 36.3 pg/mL. The immunosensor detected BNP in spiked human sera. The analytic recovery of added BNP in human sera was 97.04%. The present method was fairly consistent with commercial approach. The working electrode was stored for 2 months in cold. BSA-IgG had no interference in the electrode activity showing its high specificity for BNP. This novel approach provided a new POC-diagnostics, as direct sample measurements are easier and more efficient by this immunosensor compared to existing immunosensors.
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References
Batra B, Lata SS et al (2013) Construction of an amperometric bilirubin biosensor based on covalent immobilization of bilirubin oxidase onto zirconia coated silica nanoparticles/chitosan hybrid film. Biosens Bioelectron 44:64–69. https://doi.org/10.1016/j.bios.2012.12.034
Burcu Bahadir E, Kemal Sezgintürk M (2015) Applications of electrochemical immunosensors for early clinical diagnostics. Talanta 132:162–174. https://doi.org/10.1016/j.talanta.2014.08.063
Castiglione V, Aimo A, Vergaro G et al (2021) Biomarkers for the diagnosis and management of heart failure. Heart Fail Rev. https://doi.org/10.1007/s10741-021-10105-w
Chen X, Wang Y, Zhou J et al (2008) Electrochemical impedance immunosensor based on three-dimensionally ordered macroporous gold film. Anal Chem 80:2133–2140. https://doi.org/10.1021/ac7021376
Dahiya T, Yadav S, Yadav N et al (2021) Monitoring of BNP cardiac biomarker with major emphasis on biosensing methods: a review. Sensors Int 2:100103. https://doi.org/10.1016/j.sintl.2021.100103
Dahiya T, Ravina MA et al (2023) Impedimetric immunosensor based on chitosan-modified gold wire with Au@rGO nanocomposite for the detection of brain natriuretic peptide (BNP). J Mater Sci. https://doi.org/10.1007/s10853-023-08311-y
Deepa PS, Pundir CS (2020) Detection of tumor suppressor protein p53 with special emphasis on biosensors: a review. Anal Biochem 588:113473. https://doi.org/10.1016/j.ab.2019.113473
Deepa NB, Chaudhary R, Pundir CS (2022) Amperometric detection of tumor suppressor protein p53 via pencil graphite electrode for fast cancer diagnosis: p53 targeted immunosensor for early diagnosis of cancer. Anal Biochem 639:114528. https://doi.org/10.1016/j.ab.2021.114528
Deswal R, Narwal V, Dang AS, Pundir CS (2019) An ultrasensitive electrochemical immunosensor for detection of sex hormone binding globulin. Microchem J 149:104010. https://doi.org/10.1016/j.microc.2019.104010
Dong X, Huang W, Chen P (2011) In situ synthesis of reduced graphene oxide and gold nanocomposites for nanoelectronics and biosensing. Nanoscale Res Lett 6:1–6. https://doi.org/10.1007/s11671-010-9806-8
Gaggin HK, Januzzi JL (2013) Biomarkers and diagnostics in heart failure. Biochim Biophys Acta Mol Basis Dis 1832:2442–2450. https://doi.org/10.1016/j.bbadis.2012.12.014
Grabowska I, Sharma N, Vasilescu A et al (2018) Electrochemical aptamer-based biosensors for the detection of cardiac biomarkers. ACS Omega 3:12010–12018. https://doi.org/10.1021/acsomega.8b01558
Habte AT, Ayele DW, Hu M (2019) Synthesis and characterization of reduced graphene oxide (rGO) started from graphene oxide (GO) using the tour method with different parameters. Adv Mater Sci Eng. https://doi.org/10.1155/2019/5058163
Hartati YW, Daniswara MH, Nurmalasari R et al (2018) An Electrochemical immunosensor for the detection of B-type natriuretic peptide (BNP) based on sandwich ELISA using screen printed carbon electrodes. Molekul 13:1. https://doi.org/10.20884/1.jm.2018.13.1.379
He X, Yuan R, Chai Y, Shi Y (2008) A sensitive amperometric immunosensor for carcinoembryonic antigen detection with porous nanogold film and nano-Au/chitosan composite as immobilization matrix. J Biochem Biophys Methods 70:823–829. https://doi.org/10.1016/j.jbbm.2007.06.002
He LH, Xue R, Yang DB et al (2009) Effects of blending chitosan with peg on surface morphology, crystallization and thermal properties. Chin J Polym Sci (english Ed) 27:501–510. https://doi.org/10.1142/S0256767909004175
Hong B, Kang KA (2006) Biocompatible, nanogold-particle fluorescence enhancer for fluorophore mediated, optical immunosensor. Biosens Bioelectron 21:1333–1338. https://doi.org/10.1016/j.bios.2005.04.007
Kaya SI, Cetinkaya A, Ozcelikay G et al (2023) Approaches and challenges for biosensors for acute and chronic heart failure. Biosensors 13:1–17. https://doi.org/10.3390/bios13020282
Landim VPA, Silva BVM, Sobral Filho DC, Dutra RF (2021) A novel redox-free immunosensor concept based on cobalt phthalocyanine@carbon nanotubes pseudocapacitor for cardiac B-type natriuretic peptide detection. Electroanalysis 33:2302–2309. https://doi.org/10.1002/elan.202100177
Liu P, Huang J, Sanchez DVP et al (2016) High yield two-dimensional (2-D) polyaniline layer and its application in detection of B-type natriuretic peptide in human serum. Sens Actuators B Chem 230:184–190. https://doi.org/10.1016/j.snb.2016.02.051
Maalouf R, Bailey S (2016) A review on B-type natriuretic peptide monitoring: assays and biosensors. Heart Fail Rev 21:567–578. https://doi.org/10.1007/s10741-016-9544-9
Maddinedi SB, Mandal BK, Vankayala R et al (2014) Casein mediated green synthesis and decoration of reduced graphene oxide. Spectrochim Acta Part A Mol Biomol Spectrosc 126:227–231. https://doi.org/10.1016/j.saa.2014.01.114
Magnussen C, Blankenberg S (2018) Biomarkers for heart failure: small molecules with high clinical relevance. J Intern Med 283:530–543. https://doi.org/10.1111/joim.12756
Matsuura H, Sato Y, Niwa O, Mizutani F (2005) Surface electrochemical enzyme immunoassay for the highly sensitive measurement of B-type natriureric peptide. Sens Actuators B Chem 108:603–607. https://doi.org/10.1016/j.snb.2004.11.042
Movahed SK, Fakharian M, Dabiri M, Bazgir A (2014) Gold nanoparticle decorated reduced graphene oxide sheets with high catalytic activity for Ullmann homocoupling. RSC Adv 4:5243–5247. https://doi.org/10.1039/c3ra45518a
Ponikowski P, Anker SD, AlHabib KF et al (2014) Heart failure: preventing disease and death worldwide. ESC Heart Fail 1:4–25. https://doi.org/10.1002/ehf2.12005
Sarangadharan I, Wang SL, Tai TY et al (2018) Risk stratification of heart failure from one drop of blood using hand-held biosensor for BNP detection. Biosens Bioelectron 107:259–265. https://doi.org/10.1016/j.bios.2018.02.036
Serafín V, Torrente-Rodríguez RM, González-Cortés A et al (2018) An electrochemical immunosensor for brain natriuretic peptide prepared with screen-printed carbon electrodes nanostructured with gold nanoparticles grafted through aryl diazonium salt chemistry. Talanta 179:131–138. https://doi.org/10.1016/j.talanta.2017.10.063
Szunerits S, Mishyn V, Grabowska I, Boukherroub R (2019) Electrochemical cardiovascular platforms: current state of the art and beyond. Biosens Bioelectron 131:287–298
Taniselass S, Arshad MKM, Gopinath SCB et al (2021) Impedimetric cardiac biomarker determination in serum mediated by epoxy and hydroxyl of reduced graphene oxide on gold array microelectrodes. Microchim Acta 188:1–13. https://doi.org/10.1007/s00604-021-04922-x
Uygun ZO, Ertuǧrul Uygun HD (2014) A short footnote: circuit design for faradaic impedimetric sensors and biosensors. Sens Actuators B Chem 202:448–453. https://doi.org/10.1016/j.snb.2014.05.029
Van Kimmenade RRJ, Januzzi JL (2012) Emerging biomarkers in heart failure. Clin Chem 58:127–138. https://doi.org/10.1373/clinchem.2011.165720
Vasantham S, Alhans R, Singhal C et al (2020) Paper based point of care immunosensor for the impedimetric detection of cardiac troponin I biomarker. Biomed Microdevices. https://doi.org/10.1007/s10544-019-0463-0
Wang H, Ma Z, Han H (2019) A novel impedance enhancer for amperometric biosensor based ultrasensitive detection of matrix metalloproteinase-2. Bioelectrochemistry 130:107324. https://doi.org/10.1016/j.bioelechem.2019.06.009
Wang L, Han Y, Wang H et al (2021) A MXene-functionalized paper-based electrochemical immunosensor for label-free detection of cardiac troponin I. J Semicond. https://doi.org/10.1088/1674-4926/42/9/092601
Wu Y, Zheng J, Li Z et al (2009) A novel reagentless amperometric immunosensor based on gold nanoparticles/TMB/Nafion-modified electrode. Biosens Bioelectron 24:1389–1393. https://doi.org/10.1016/j.bios.2008.07.075
Zhang Y, Zhang K, Ma H (2009) Electrochemical DNA biosensors based on gold nanoparticles/cysteamine/poly(glutamic acid) modified electrode. Am J Biomed Sci. https://doi.org/10.5099/aj090200115
Zhao J, Liang D, Gao S et al (2019) Analyte-resolved magnetoplasmonic nanocomposite to enhance SPR signals and dual recognition strategy for detection of BNP in serum samples. Biosens Bioelectron. https://doi.org/10.1016/j.bios.2019.11144
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The authors are thankful to Aryabhatta Central Instrumentation Laboratory (ACIL) of M.D. University, Rohtak for providing potentiostat facility for this work.
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Dahiya, T., Sharma, M., Rathee, R. et al. An impedimetric immunosensor based on chitosan–Au nanoparticles-reduced graphene oxide nanosheet composite modified PG electrode for detection of brain natriuretic peptide. 3 Biotech 13, 280 (2023). https://doi.org/10.1007/s13205-023-03704-x
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DOI: https://doi.org/10.1007/s13205-023-03704-x