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Development of eight microsatellite loci from the endangered huemul (Hippocamelus bisulcus) and cross-species amplification in six other ungulate species

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Abstract

We developed eight polymorphic microsatellite loci for the huemul (Hippocamelus bisulcus) and tested for cross-species amplification in six other ungulate species. Microsatellite loci were typed in 58 individuals from the Lago Cochrane National Reserve, Chile. Two to five alleles were observed per locus (mean 3.4) and observed heterozygosity ranged from 0.29 to 0.60 (mean 0.53). One to five loci amplified in other ungulate species. These markers will be used to refine studies of population structure and allow for more accurate pedigree reconstruction in the huemul.

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Acknowledgments

Microsatellite identification and primer design were part of the Biology 592 class project at the University of Alberta; specifically, we wish to thank Kelsey Bernard, Heather Bird and Wentong Long. We thank the Department of Biological Sciences the Molecular Biology Facility (MBSU) at the University of Alberta for their support. Thanks to all those involved in sample collection (listed in Corti et al. 2011).

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Authors and Affiliations

  1. Department of Biological Sciences, University of Alberta, Edmonton, AB, T6G 2E9, Canada

    Aaron B. A. Shafer, David W. Coltman & Corey S. Davis

  2. Instituto de Ciencia Animal, Programa de Investigación Aplicada en Fauna Silvestre, Facultad de Ciencias Veterinarias, Universidad Austral de Chile, Casilla 567, Valdivia, Chile

    Paulo Corti

  3. Departamento de Ciencias Básicas, Facultad de Ciencias, Universidad del Bío-Bío, Chillán, Chile

    Juan Carlos Marín

Authors
  1. Aaron B. A. Shafer
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  2. Paulo Corti
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  3. David W. Coltman
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  4. Juan Carlos Marín
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  5. Corey S. Davis
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Correspondence to Corey S. Davis.

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Shafer, A.B.A., Corti, P., Coltman, D.W. et al. Development of eight microsatellite loci from the endangered huemul (Hippocamelus bisulcus) and cross-species amplification in six other ungulate species. Conservation Genet Resour 4, 571–573 (2012). https://doi.org/10.1007/s12686-011-9594-1

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  • DOI: https://doi.org/10.1007/s12686-011-9594-1

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