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Study on the purification of polysaccharides from Noscoc flagelliforme with radial flow chromatography

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An Erratum to this article was published on 01 August 2009

Abstract

The isolation and purification of polysaccharide from Noscoc flagelliforme by radial flow chromatography were studied. The column (7.7 cm of bed length and 229.6 cm3 of bed volume) was packed with DEAE-01 anion ion-exchange resin and gradient eluted with NaCl solutions. The content of the polysaccharide was determined with the phenol-sulfuric acid method. The effects of sampling weight, elution velocity, and elution concentration gradient on the separation efficiency were examined and three isolated peaks were obtained. The optimal separation conditions are 10 mg of the sampling weight (sampling volume is 20 mL), 1.0 mL/min of the elution velocity, and 1.00 mol/L2 of NaCl gradient elution. The adjacent peak resolutions among the three main components (1, 2, and 3 according to their elution order) are 0.660 (R 12) and 0.786 (R 23), respectively. It is deduced that 39.8 cm of the bed length is required for the fully separation of the three polysaccharides.

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Reference

  1. Hopf, P. P. (1947) Radial chromatography in industry. Ind. Eng. Chem. 39: 938–940.

    Article  CAS  Google Scholar 

  2. Heftmann, E., J. M. Krochta, D. F. Farkas, and S. Schwimmer (1972) The chromatofuge, an apparatus for preparative rapid radial column chromatography. J. Chromatogr. 66: 365–369.

    Article  CAS  Google Scholar 

  3. McCormick, D. (1988) Chromatograph and affinity separations report. Bio/Technol. 6: 158–165.

    Article  Google Scholar 

  4. Scott, R. P. W. (2003) Preparative chromatography. Chrom-Ed Book Series, Chrom. Book 12, Library-4science, LLC, Riyadh, Saudi Arabia.

    Google Scholar 

  5. Gu, T., G. J. Tsai, and G. T. Tsao (1991) A theoretical study of multicomponent radial flow chromatography. Chem. Eng. Sci. 46: 1279–1288.

    Article  CAS  Google Scholar 

  6. Saxena, V. and M. Dunn (1989) Solving scale-up: Radial flow chromatography. Bio/Technol. 7: 250–255.

    Article  Google Scholar 

  7. Levison, P. R (2003) Large-scale ion-exchange column chromatography of proteins: Comparison of different formats. J. Chrom. B 790: 17–33.

    Article  CAS  Google Scholar 

  8. Lay, M. C., C. J. Fee, and J. E. Swan (2006) Continuous radial flow chromatography of proteins. Food Bioprod. Proc. 84: 78–83.

    Article  CAS  Google Scholar 

  9. Jungbauer, A., D. Pettauer, A. Buchacher, E. Wenisch, F. Unterluggauer, K. Uhl, and F. Steindl (1988) Scaleup of monoclonal-antibody purification using radial streaming ion-exchange chromatography. Biotechnol. Bioeng. 32: 326–333.

    Article  CAS  Google Scholar 

  10. Kim, Y. H. and E. K. Lee (1996) Comparison of axial and radial flow chromatography on protein separation speed and resolution. Korean J. Chem. Eng. 13: 466–472.

    Article  CAS  Google Scholar 

  11. Sun, T., G. Chen, Y. Liu, F. Bu, and M. Wen (2000) Purification of human prothrombin from Nitschmann fraction III using DEAE membrane radial flow chromatography. J. Chrom. B: Biomed. Sci. Appl. 742: 109–114.

    Article  CAS  Google Scholar 

  12. Planques, Y. and H. P. D. Menozzi (1991) Affinity purification of plasminogen by radial-flow affinity chromatography. J. Chromatogr. A 539: 531–533.

    Article  CAS  Google Scholar 

  13. Tharakan, J. and M. Belizaire (1995) Ligand efficiency in axial and radial flow immunoaffinity chromatography of factor IX. J. Chromatogr. A 702: 191–196.

    Article  CAS  Google Scholar 

  14. Saxena, V. and A. E. Weil (1987) Radial flow columns: a new approach to scaling-up biopurifications. Biochromatogr. 2: 90–97.

    CAS  Google Scholar 

  15. Gao, K. S. (1998) Chinese studies on the edible luegreen alga, N. flagelliforme. J. Appl. Phycol. 10: 37–49.

    Article  Google Scholar 

  16. Knubel, G., L. K. Larsen, R. E. Moore, I. A. Levine, and G. M. L. Patterson (1990) Cytotoxic, antiviral indolocarbazoles from a blue-green alga belonging to the Nostoc. J. Antibiot. 43: 1236–1239.

    CAS  Google Scholar 

  17. Kanekiyo, K., J. B. Lee, K. Hayashi, H. Takenaka, Y. Hayakawa, S. Endo, and T. Hayashi (2005) Isolation of an antiviral polysaccharide nostoflan from a terrestrial cyanobacterium N. flagelliforme. J. Nat. Prod. 68: 1037–1041.

    Article  CAS  Google Scholar 

  18. Jia, S. R., H. F. Yu, Y. X. Lin, and Y. J. Dai (2007) Characterization of extracellular polysaccharides from N. flagelliforme cells in liquid suspension culture. Biotechnol. Bioprocess Eng. 12: 271–275.

    Article  CAS  Google Scholar 

  19. Yu, H. F., S. R. Jia, and Y. J. Dai (2008) Growth characteristics of the cyanobacterium N. flagelliforme in photoautotrophic, mixotrophic, and heterotrophic cultivation. J. Appl. Phycol. DOI/10.1007/s10811-008-9341-5.

  20. Su, J. Y., S. R. Jia, X. F. Chen, and H. F. Yu (2008) Morphology, cell growth, and polysaccharide production of N. flagelliforme in liquid suspension culture at different agitation rates. J. Appl. Phycol. 20: 213–217.

    Article  CAS  Google Scholar 

  21. Methods for pretreating ion exchange resins: GB 5476-85 (1986)

  22. Miller, G. L. (1954) Use of dinitrosalicylic acid reagent for determination of reducing sugar. Anal. Chem. 31: 426–428

    Article  Google Scholar 

  23. Scott, R. P. W. (1992) Liquid chromatography column theory. 2nd ed. John Wiley & Sons, NY, USA.

    Google Scholar 

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Authors and Affiliations

  1. Key Laboratory of Industrial Microbiology, Ministry of Education, College of Biotechnology, Tian** University of Science and Technology, Tian**, 300-457, China

    Yu-Jie Dai, **g-Wen Wang, Shi-Ru Jia, Si-Jun Yue, Meng-Yao Jia & Peng Xu

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  1. Yu-Jie Dai
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  2. **g-Wen Wang
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  3. Shi-Ru Jia
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  4. Si-Jun Yue
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  5. Meng-Yao Jia
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  6. Peng Xu
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Correspondence to Yu-Jie Dai.

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An erratum to this article can be found at http://dx.doi.org/10.1007/s12257-009-1001-3

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Dai, YJ., Wang, JW., Jia, SR. et al. Study on the purification of polysaccharides from Noscoc flagelliforme with radial flow chromatography. Biotechnol Bioproc E 14, 377–382 (2009). https://doi.org/10.1007/s12257-008-0210-5

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  • DOI: https://doi.org/10.1007/s12257-008-0210-5

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