Abstract
A bacterial cellulose (BC) strain was isolated from fermented grape juice. BC was confirmed using molecular identification with partial sequencing of 16S rRNA genes, which were then registered in the GenBank database as Komagataeibacter hansenii NRC9 with accession number MW366921. Factors, such as pH, temperature and incubation time, were assessed under fixed inoculum size and age using Hestrin–Schramm (HS) medium. Alterations in the carbon and nitrogen sources occurred in the components of the HS medium. The results showed that the highest wet weight of BC membranes by K. hansenii strain was obtained using glucose and yeast extract as carbon and nitrogen source, respectively, at pH 5 and incubation temperature of 35°C. The wet weight of BC increased as incubation time increased up to 30 days. The obtained BC fibres were investigated using a scanning electron microscope, transmission electron microscope (TEM) and X-ray diffraction (XRD). Results showed a coherent 3D network formed by cellulose fibres in the range of 40–60 nm. These nanofibres contact with each other in compactness to form a bacterial nano-cellulose hydrogel. TEM micrographs demonstrate long nano-fibrils in the nanometre scale, 5 nm in width. XRD spectrum confirmed the crystallinity of BC pellicle.
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We gratefully thank the National Research Centre for the financial support of the in-house Project No. 12010311.
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Sheir, D.H., Elshereef, A.A., El-Masry, H.M. et al. Biosynthesized 3D biofilm-based bacterial cellulose nanofibre using Komagataeibacter hansenii NRC9: optimization, production and characterizations. Bull Mater Sci 46, 154 (2023). https://doi.org/10.1007/s12034-023-02982-8
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DOI: https://doi.org/10.1007/s12034-023-02982-8