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Conversion ofG. hansenii PJK into non-cellulose-producing mutants according to the culture condition

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Abstract

The conversion of a cellulose-producing cell (Cel +) fromGluconacetobacter hansenii PJK (KCTC 10505 BP) to a non-cellulose-producing cell (Cel −) was investigated by measuring the colony forming unit (CFU). This was achieved in a shaking flask with three slanted baffles, which exerted a strong shear stress. The addition of organic acid, such as glutamic acid and acetic acid, induced the conversion of microbial cells from a wild type toCel − mutants in a flask culture. The supplementation of 1% ethanol to the medium containing an organic acid depressed the conversion of the microbial cells toCel − mutants in a conventional flask without slanted baffles. The addition of ethanol to the medium containing an organic acid; however, accelerated the conversion of microbial cells in the flask with slanted baffles. TheCel + cells from the agitated culture were not easily converted intoCel −, mutants on the additions of organic acid and ethanol to a flask without slanted baffles, but some portion of theCel + cells were converted toCel − mutants in a flask with slanted baffles. The conversion ratio ofCel + cells toCel − mutants was strongly related to the production of bacterial cellulose independently from the cell growth.

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Authors and Affiliations

  1. Department of Chemical Engineering, Kyungpook National University, 702-701, Daegu, Korea

    Joong Kon Park, Seung Hun Hyun & Jae Yong Jung

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  1. Joong Kon Park
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  2. Seung Hun Hyun
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  3. Jae Yong Jung
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Correspondence to Joong Kon Park.

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Park, J.K., Hyun, S.H. & Jung, J.Y. Conversion ofG. hansenii PJK into non-cellulose-producing mutants according to the culture condition. Biotechnol Bioproc E 9, 383–388 (2004). https://doi.org/10.1007/BF02933062

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  • DOI: https://doi.org/10.1007/BF02933062

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