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Triptonide, a Diterpenoid Displayed Anti-Inflammation, Antinociceptive, and Anti-Asthmatic Efficacy in Ovalbumin-Induced Mouse Model

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Abstract

The present study was intended to explore the valuable effects of triptonide on inflammation, asthmatic, and nociceptive. Triptonide possesses numerous beneficial effects extensively managed in the treatment of inflammation disease condition. Initially, triptonide showed anti-inflammation properties over lipopolysaccharide-induced RAW 264.7 cells. Hence, the present study was directed to explore the protecting efficacy of triptonide in ovalbumin (OVA)-induced asthma in mice. Asthma was induced intraperitoneally administration (200μL) in female BALB/c mice with suspension which has ovalbumin (100 μg/mL) and aluminum hydroxide (10 mg/mL). Triptonide (30 mg/kg) over OVA-induced experimental animals altered lung mass, nitric oxide, myeloperoxidase, immunoglobulin E status, interleukins (4, 5, and 13) inflammatory cytokines status, and histological modifications. Animals were also managed with the standard drug dexamethasone (50 mg/kg) followed by the asthma induction, which is also efficient over OVA-induced experimental animals. The nociception was provoked in male Swiss mice by various chemicals (acetic acid, capsaicin, and glutamate). The animals were administered with triptonide (5, 10, and 15 mg/kg) and separate standard drugs like diclofenac sodium (10 mg/kg) and morphine (5 mg/kg) over chemical-induced nociceptive animals. The present outcome evidently established that the triptonide considerably reduced the various chemical-induced nociception in mice (Fig. 7A, B, and C). Ultimately, the present work explored the evident powerful anti-inflammation, antinociceptive, and anti-asthma properties of a diterpenoid, triptonide experimental animal models. And it is recommended that triptonide is an excellent compound in the management of asthma and its related diseases.

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Data Availability

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Abbreviations

OVA :

Ovalbumin

TPN :

Triptonide

MTT :

3-(4, 5-Dimethylthiazol-2-y1)-2, 5-diphenyltetrazolium bromide

DMSO :

Dimethyl sulfoxide

DMEM :

Dulbecco’s modified Eagle’s medium

FBS :

Fetal bovine serum

LPS :

Lipopolysaccharides

MPO :

Myeloperoxidase

NO :

Nitric oxide

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Authors and Affiliations

  1. Department of Pulmonary and Critical Care Medicine, Shandong Second Provincial General Hospital, Shandong Province, **an, 250012, China

    Zhen Li

  2. Department of Respiratory Medicine, PKU Care Zibo Hospital, Shandong Province, Zibo, 255000, China

    Yanhong Geng

  3. Anser Science Joint Laboratory Platform, Anser Press Group, Shandong Province, **an, 250000, China

    Qingke Wu & **n **

  4. Department of Pharmacology and Toxicology, College of Pharmacy, Prince Sattam Bin Abdul Aziz University, Al-Kharj, Kingdom of Saudi Arabia

    Vidya Devanathadesikan Seshadri

  5. Department of Anesthesiology, **an Central Hospital, Cheeloo College of Medicine, Shandong University, Shandong Province, **an, 250014, China

    Hao Liu

  6. Department of Anesthesiology, Central Hospital Affiliated to Shandong First Medical University, Shandong Province, **an, 250014, China

    Hao Liu

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  1. Zhen Li
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Li, Z., Geng, Y., Wu, Q. et al. Triptonide, a Diterpenoid Displayed Anti-Inflammation, Antinociceptive, and Anti-Asthmatic Efficacy in Ovalbumin-Induced Mouse Model. Appl Biochem Biotechnol 195, 1736–1751 (2023). https://doi.org/10.1007/s12010-022-04167-1

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