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Unveiling the Influence of Glutathione in Suppressing the Conversion of Aspirin to Salicylic Acid: A Fluorescence and DFT Study

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Abstract

Aspirin is a commonly used nonsteroidal anti-inflammatory drug, associated with many adverse effects. The adverse effects of aspirin such as tinnitus, Reye’s syndrome and gastrointestinal bleeding are caused due to conversion of aspirin into its active metabolite salicylic acid after oral intake. Glutathione is a naturally occurring antioxidant produced by the liver and nerve cells in the central nervous system. It helps to metabolize toxins, break down free radicles, and support immune function. This study aims to investigate and explore the possibility of inhibiting aspirin to salicylic acid conversion in presence of glutathione at a molecular level using spectroscopic techniques such as UV–Visible absorption, time-Resolved and time-dependent fluorescence and theoretical DFT/ TD-DFT calculations. The results of steady state fluorescence spectroscopy and time-dependent fluorescence indicated that the aspirin to salicylic acid conversion is considerably inhibited in presence of glutathione. Further, the results presented here might have significant clinical implications for individuals with variations in glutathione level.

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No datasets were generated or analysed during the current study.

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Acknowledgements

Monalisha Nayak would like to acknowledge UGC New Delhi for providing non net fellowship. Prof Ranjan K Singh is thankful to DST- FIST programme, and ongoing BHU IOE scheme for financial support. Authors are thankful to Prof Amit Pathak for providing computational facility.

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Authors and Affiliations

  1. Department of Physics, Institute of Science, Banaras Hindu University, Varanasi, 221005, India

    Monalisha Nayak, Chandan Bhai Patel, Anurag Mishra, Ranjana Singh & Ranjan K. Singh

  2. Government Polytecnic Rajgrah, Mirzapur, Bathua, 231001, India

    Ranjana Singh

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  1. Monalisha Nayak
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Contributions

Monalisha Nayak: Designing and execution of ideas for sample preparation, characterization, theoretical calculation as well as manuscript writing, Chandan Bhai Patel: Sample preparation, discussion of the results, review of the manuscript, Anurag Mishra: Experimental suggestion, Ranjana Singh: Ideas, experiments, results and discussion, manuscript writing. Ranjan K. Singh: Supervision for the experiment, result and discussion, manuscript writing, reviewing and editing.

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Nayak, M., Patel, C.B., Mishra, A. et al. Unveiling the Influence of Glutathione in Suppressing the Conversion of Aspirin to Salicylic Acid: A Fluorescence and DFT Study. J Fluoresc 34, 1441–1451 (2024). https://doi.org/10.1007/s10895-024-03665-1

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