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Stress response mechanisms and description of three novel species Shewanella avicenniae sp. nov., Shewanella sedimentimangrovi sp. nov. and Shewanella yunxiaonensis sp. nov., isolated from mangrove ecosystem

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Abstract

Three Gram-staining negative, facultatively anaerobic, rod-shaped and motile strains, FJAT-51800T, FJAT-52962T and FJAT-54481T were isolated from the sediment samples of Zhangjiang Estuary Mangrove National Nature Reserve in Fujian Province, China. The 16S rRNA gene sequencing results indicated they could be novel members of the genus Shewanella. The optimum temperature for growth was 30 °C. The respiratory quinones of the strains were ubiquinone Q-7 or Q-8, and menaquinone MK-7. Polar lipids of the strains FJAT-52962T and FJAT-51800T were phosphatidyl glycerol, phosphatidyl ethanolamine, and unidentified aminophospholipids while strain FJAT-54481 consist of phosphatidylglycerol, phosphatidylethanolamine, unidentified aminophospholipids, two unidentified aminolipids and four unidentified lipids. The major fatty acid of the three strains was iso-C15:0. The genomic DNA G + C contents of strains FJAT-51800T, FJAT-52962T and FJAT-54481T were 48.2, 55.3 and 48.1%, respectively. The average nucleotide identity and digital DNA–DNA hybridization values between strains FJAT-51800T, FJAT-52962T and FJAT-54481T and other closely related Shewanella members were below the cut-off level (95–96%) for species identification. Genome analysis showed that these strains encode genes for osmo-regulation. Based on the results of phenotypic, chemotaxonomic and genome analyses, strains FJAT-51800T, FJAT-52962T and FJAT-54481T represent three novel species of the genus Shewanella, for which the names Shewanella avicenniae sp. nov., Shewanella sedimentimangrovi sp. nov., and Shewanella yunxiaonensis sp. nov., are proposed. The type strains are FJAT-51800T (= GDMCC 1.2204T = KCTC 82448T), FJAT-52962T (= MCCC 1K05496T = KCTC 82445T) and FJAT-54481T (= GDMCC 1.2348T = KCTC 82646T).

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Acknowledgements

We thank Aharon Oren for nomenclature help.

Funding

This work was supported by grants from Fujian Special Fund for Scientific Research Institutes in the Public Interest (2020R1034001), Fujian Academy of Agricultural Sciences (GJPY2019003), and also by Innovation Group Project of Southern Marine Science and Engineering Guangdong Laboratory (Zhuhai) (No. 311021006).

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Author notes

  1. Guo-Hong Liu, Qi Zhang, Manik Prabhu Narsing Rao, have contributed equally to this work.

Authors and Affiliations

  1. Agricultural Bio-Resources Research Institute, Fujian Academy of Agricultural Sciences, Fuzhou, Fujian, 350003, People’s Republic of China

    Guo-Hong Liu, Qi Zhang, Shang Yang, Rong Tang, Huai Shi, Jie-** Wang & Bo Liu

  2. Fujian Provincial Key Laboratory of Soil Environmental Health and Regulation, College of Resources and Environment, Fujian Agricultural and Forestry University, Fuzhou, Fujian, 350002, People’s Republic of China

    Qi Zhang, Shang Yang, Rong Tang & Shun-Gui Zhou

  3. State Key Laboratory of Biocontrol, Guangdong Provincial Key Laboratory of Plant Resources and Southern Marine Science and Engineering Guangdong Laboratory (Zhuhai), School of Life Sciences, Sun Yat-Sen University, Guangzhou, 510275, People’s Republic of China

    Manik Prabhu Narsing Rao & Wen-Jun Li

  4. Administrative Bureau of Zhangjiang Estuary Mangrove National Nature Reserve Yunxiao Town, Yunxiao, Fujian, 363300, People’s Republic of China

    Guan-Min Huang

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  1. Guo-Hong Liu
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Contributions

GHL, QZ and MPNR designed the experiments and wrote the manuscript. SY, RT and HS performed the genome analysis. BL revised the manuscript. JPW and GMH performed the part of biochemical characterization. SGZ and WJL supervised the experiments.

Corresponding authors

Correspondence to Shun-Gui Zhou or Wen-Jun Li.

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The authors declare that there are no conflicts of interest.

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This article does not contain any studies with human participants or animals performed by any of the authors.

Data availability

The GenBank/EMBL/DDBJ accession numbers for the 16S rRNA gene sequences of strains FJAT-54481T, FJAT-52962T and FJAT-51800T are MW217203, MW073524 and MW073471, respectively. The GenBank/EMBL/DDBJ accession numbers for the genome sequences are CP073587, CP071502 and CP071503, respectively.

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Liu, GH., Zhang, Q., Narsing Rao, M.P. et al. Stress response mechanisms and description of three novel species Shewanella avicenniae sp. nov., Shewanella sedimentimangrovi sp. nov. and Shewanella yunxiaonensis sp. nov., isolated from mangrove ecosystem. Antonie van Leeuwenhoek 114, 2123–2131 (2021). https://doi.org/10.1007/s10482-021-01666-9

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