Abstract
Background
Peritoneal fibrosis (PF) is caused by epithelial-mesenchymal transdifferentiation (EMT) in the peritoneum under high glucose (HG) conditions. The study aimed to explored the role of Insulin-like growth factor 1 receptor (IGF-1R) in the regulation of EMT in human peritoneal mesothelial cells (HPMCs).
Methods
We used HG peritoneal dialysis fluid (PDF) to induce in vivo PF in mice, and treated HPMCs with HG in vitro to stimulate EMT.
Results
In the mice, the higher the glucose concentration in the dialysate, the more obvious the peritoneal tissue thickening and the more that collagen was deposited. The in vitro study indicated that the expression of IGF-1R, α-SMA, vimentin was upregulated, while the expression of occludin, ZO-1, and E-cadherin was downregulated in HPMCs under HG and IGF-1R overexpression conditions. Conversely, the expression of IGF-1R, α-SMA, and vimentin was downregulated, while the expression of occludin, ZO-1, and E-cadherin was upregulated in IGF-1R-underexpressed HPMCs under HG conditions. The cell migration abilities were increased, while the cell adhesion abilities were reduced in HPMCs under HG and IGF-1R overexpression conditions. In contrast, cell migration abilities were reduced, while cell adhesion abilities were increased in IGF-1Runderexpressed HPMCs under HG conditions.
Conclusions
Targeting at IGF-1R may provide novel insights into the prevention and treatment of PF.
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Acknowledgements
This work was supported by the General Project of Nan**g Medical Science and Technology Development (YKK19057).
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**a, Y., Wan, C., Zhang, Q. et al. Role of IGF-1R in epithelial–mesenchymal transdifferentiation of human peritoneal mesothelial cells. Clin Exp Nephrol 26, 630–639 (2022). https://doi.org/10.1007/s10157-022-02209-w
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DOI: https://doi.org/10.1007/s10157-022-02209-w