Abstract
Recombinant eel GH and yeast containing chinook salmon growth hormone (reGH and rcsGH) were incorporated into gelatin and sodium alginate (reGH-GS and rcsGH-GS) or polymer matrix (reGH-HP55) to protect the hormone from proteolytic cleavage in the stomach. The diets containing reGH-GS, rcsGH-GS, reGH-HP55 and free-reGH or uncoated-rcsGH were administered to red sea bream. Feeding of reGH-GS, reGH-HP55 and rcsGH-GS diets resulted in significant increases in body weight and fork length over those of controls. These results strongly suggest that gelatin and sodium alginate as well as polymer matrix protected the hormone from proteolytic enzymes in the gastrointestinal tract to allow the bioactive hormone to enter the circulation and eventually stimulate fish growth.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Adelman, I. R. 1977. Effects of bovine growth hormone on growth of carp (Cyprinus carpio) and the influences of temperature and photoperiod.J. Fish Res Board Can. 34: 509–511.
Agellon, L. B., Emery, C. J., Jone, J. M. et al., 1988. Promotion of rapid growth of rainbow trout (Salmon gairdneri) by a recombinant fish growth hormone.Can. J. Fish. Aquat. Sci. 45: 146–151.
Brown, M. E., 1957. The Physiology of Fishes. Vol. I. Metabolism. Academic Press, New York, NY, p. 361–400.
Down, N. E., Donaldson, E. M., Dye, H. M. et al., 1988. Recombinant bovine somatotropin more than doubles the growth rate of coho salmon (Oncorhynchus kisutch) acclimated to sea water and ambient winter conditions.Aquaculture 68: 141–155.
Duan, C., Hirano, T., 1991. Plasma kinetics of growth hormone in the Japanese eel.Anguilla japonica, Aquaculture 95: 179–188.
Hertz, Y., Tchelet, A., Madar, Z. et al., 1991. Absorption of bioactive human growth hormone after oral administration in the common carp (Cyprinus carpio) and its enhancement by deoxycholate.J. Comp. physiol. 161B: 159–163.
Higgs, D. A., Donaldson, E. M., Dye, H. M., et al., 1976. Influence of bovine growth hormone and L-thyroxine on growth, muscle composition and histological structure of the gonads, thyroids, pancreas and pituitary of the coho salmon (Oncorhynchus kisutch).J. Fish. Res. Board Can. 33: 1583–1603.
Kawauchi, H., Moriyama, S., Yasuda, A. et al., 1986. Isolation and characterization of chum salmon growth hormone.Arch. Biochem. Biophys. 244: 542–552.
Kawauchi, H., Moriyama, S., 1988. Chum salmon growth hormone: isolation and effects on growth of juvenile rainbow trout.In: Spark, A. K. ed., New and Innovative Advances in Biology/Engineering with Potential for Use in Aquaculture. NOAA Tech. Rep. NMFS 70, Natl. Mar. Fish., p. 1–6.
LeBail, P. Y., Sire, M. F., Vernier, J. M., 1989. Intestinal transfer of growth hormone into the circulation system of rainbow trout,Salmo gairdneri: interference by granule cells.J. Exp. Zool. 251: 101–110.
McLean, E., Donaldson, E. M., 1990. Absorption of bioactive proteins by the gastrointestinal tract of fish: a review.J. Aquatic Anim. Health 2: 1–11.
McLean, E., Donaldson, E. M., Dye, H. M. et al., 1990. Growth acceleration of coho salmon (Oncorhynchus kisutch) following oral administration of recombinant bovine growth hormone.Aquaculture 91: 197–203.
Moriyama, S., Hirano, T., Kawauchi, H. 1989. Intestinal uptake of recombinant salmon growth hormone following intragastric or rectal administration to rainbow trout,Oncorhynchus mykiss.In: Miyachi, S., Kurube, I. and Ishida, U. eds, Current Topics in Marine Biotechnology. The Japanese Society for Marine Biotechnology, Tokyo, Japan, p. 299–302.
Moriyama, S., Kawauchi, H., 1990. Growth stimulation of juvenile salmonids by immersion in recombinant salmon growth hormone.Nippon. Suisan. Gakkaishi. 56(1): 31–34.
Moriyama, S., Takahashi, A., Hirano, T. et al., 1990. Salmon growth hormone is transported into the circulation of rainbow trout,Oncorhynchus mykiss, after intestinal administration.J. Comp. Physiol. 160B: 251–257.
Moriyama, S., Yamamoto, H., Sugimoto, S. et al., 1993. Oral administration of recombinant salmon growth hormone to rainbow trout,Oncorhynchus mykiss. Aquaculture 112: 99–106.
Sakine, S., Mizukami, Y., Nishi, T. et al., 1985. Cloning and expression of cDNA for salmon growth hormone inEscherichia coli.Proc. Ncad. Sic. USA 82: 4306–4310.
Schulte, P. M., Down, N. E., Donaldson, E. M. et al., 1989. Experimental administration of recombinant growth hormone to juvenile rainbow trout (Salmon gairdneri) by injection or by immersion.Aquaculture 76: 145–156.
Xu, B., Ge, G., Miao, H., 1995. The effects of recombinant eel growth hormone, methyltestosterone and L-thyroxine on the red seabream,Pagrosomus major.Chin. J. Oceanol. Limnol. 13(1): 86–96.
Xu, B., Zhang, P., Li, D. 1997a. Advances in studies on the isolation, characterization and physiological functions of fish growth hormone.Oceanol. Limnol. Sin. 28(2): 209–214. (in Chinese with English abstract)
Xu, B., Zhang, P., Li, D. 1997b. Advances in studies on the plasma metabolism, regulation and action mechanisms of fish growth hormone.Oceanol. Limnol. Sin. 28(3): 328–333. (in Chinese with English abstract)
Xu, B., Zhang, P., Li, D. 1997c. Advances in studies on molecular biology and applications of fish growth hormone.Oceanol. Limnol. Sin. 28(5): 553–557. (in Chinese with English abstract)
Author information
Authors and Affiliations
Additional information
This study was supported by a grant (No. 96D06059) from the Natural Science Foundation of Shandong Province, China.
Rights and permissions
About this article
Cite this article
Xu, B., Mai, Ks., Xu, Yl. et al. Growth promotion of red sea bream,Pagrosomus major, by oral administration of recombinant eel and salmon growth hormone. Chin. J. Ocean. Limnol. 19, 141–146 (2001). https://doi.org/10.1007/BF02863038
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF02863038