Abstract
The complete nucleotide sequence (155,844 bp) of tobacco chloroplast DNA has been determined. It contains two copies of an identical 25,339 bp inverted repeat, which are separated by a 86,684 bp and a 18,482 bp single-copy region. The genes for four different rRNAs, 30 different tRNAs, and 44 different proteins have been located. Among them, 15 different genes contain introns. Blot hybridization revealed that all rRNA, tRNA and protein genes are transcribed in the chloroplast and that primary transcripts of the split genes are spliced. Six sequences coding for proteins homologous to components of the respiratory-chain NADH dehydrogenase from human mitochondria have been found. Two sequences which autonomously replicate in yeast have also been mapped. A unique split gene,rps12, which requires trans-splicing has been identified. The sequence and expression analyses indicate both prokaryotic and eukaryotic features of the chloroplast genes.
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Sugiura, M. Structure and function of the tobacco chloroplast genome. Bot Mag Tokyo 100, 407–436 (1987). https://doi.org/10.1007/BF02488858
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DOI: https://doi.org/10.1007/BF02488858