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Physical map** of Nicotiana tabacum chloroplast DNA

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Summary

Nicotiana tabacum chloroplast DNA was digested with several restriction endonucleases chosen for their potential usefulness in distinguishing between species of the genus, Nicotiana. The resulting fragments were ordered into a circular configuration of about 160 kilobase pairs, equalling about 100x106 daltons. The physical map features an inverted, repeated unit of about 24 kilobase pairs separated by a unique sequence region with a mean size of 13 kilobase pairs on the short side. The cistrons coding for chloroplast ribosomal RNA are contained within the inverted repeat and have the arrangement: 16S, spacer, 23S, 4.5S/5S. Restriction endonuclease maps obtained with the enzymes PvuII, XhoI, and BglI are presented.

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Authors and Affiliations

  1. Department of Plant Genetics, Weizmann Institute of Science, Rehovot, Israel

    Robert Fluhr & Marvin Edelman

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  1. Robert Fluhr
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  2. Marvin Edelman
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Communicated by G. O'Donovan

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Fluhr, R., Edelman, M. Physical map** of Nicotiana tabacum chloroplast DNA. Molec. Gen. Genet. 181, 484–490 (1981). https://doi.org/10.1007/BF00428740

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  • DOI: https://doi.org/10.1007/BF00428740

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