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Targeted Mutagenesis
Mutational analysis in forward genetics is an effective strategy in invertebrate model animals and zebrafish in vertebrates. Forward genetic studies...
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CRISPR/Cas9-assisted ssDNA recombineering for site-directed mutagenesis and saturation mutagenesis of plasmid-encoded genes
Site-directed and saturation mutagenesis are critical DNA methodologies for studying protein structure and function. For plasmid-based gene mutation,...
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Random Mutagenesis by PCR
Random mutagenesis of DNA sequences has the advantage of generating DNA sequences with novel properties, either directly in the case of aptamers or...
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Transposable Elements II: Insertional Mutagenesis
This chapter is a sequel to the topic of transposable elements discussed in Chap. 5 . Here, we will...
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Loss-of-Function Mutagenesis for Forward Genetics
Genes are central players regulating biological processes. A general strategy for studying gene function is to impair gene activity and examine its...
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Mutagenesis: Exploring Genetic Diversity of Industrial Crop Plants
Beyond their use in food production, industrial crops—a distinct class of crops grown primarily for nonhuman consumption—are essential to many facets...
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CRIMP: a CRISPR/Cas9 insertional mutagenesis protocol and toolkit
Site-directed insertion is a powerful approach for generating mutant alleles, but low efficiency and the need for customisation for each target has...
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Research Progress of ARTP Mutagenesis Technology Based on Citespace Visualization Analysis
Atmospheric and room temperature plasma (ARTP) mutagenesis technology has been developed rapidly in recent years because of its simple operation,...
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Mutagenesis and Transgenesis in Plant Breeding
Mutagenesis gives an avenue of possibilities for develo** improved cultivars in crops with limited or no variability in a specific trait....
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A Cas3-base editing tool for targetable in vivo mutagenesis
The generation of genetic diversity via mutagenesis is routinely used for protein engineering and pathway optimization. Current technologies for...
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Directed mutagenesis in plants through genome editing using guide RNA library
Plants with genetic diversity have a higher rate of resilience and adaptation to environmental change. Crop species need to diversify genetically if...
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Application of Mutagenesis and Genome Editing in Crop Plants
Mutagenesis and genome editing has emerged as a substitute to traditional plant breeding and transgenics for crop improvement and hence ensure...
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Physical Mutagenesis in Cereal Crops
The use of physical mutagens to induce heritable genetic variation in crop plants dates back to the beginning of the twentieth century. While X-rays...
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Alternative Mechanisms of Mutagenesis at mCpG Sites during Replication and Repair
Abstract5-Methyl-2'-deoxycytidine (mC) at CpG sites plays a key role in the epigenetic gene regulation, cell differentiation, and carcinogenesis....
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Revisiting mutagenesis at non-B DNA motifs in the human genome
Non-B DNA structures formed by repetitive sequence motifs are known instigators of mutagenesis in experimental systems. Analyzing this phenomenon...
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In Vitro Site Directed Mutagenesis
Site-Directed Mutagenesis (SDM) allows for changes in the DNA sequence of plasmids using polymerase chain reaction (PCR). It is a reliable,...
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Induced Mutagenesis for Crop Improvement
Induced mutagenesis is one of the most powerful strategies for creating genetic diversity and identifying critical regulatory genes for economically...
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Single-mitosis dissection of acute and chronic DNA mutagenesis and repair
How chronic mutational processes and punctuated bursts of DNA damage drive evolution of the cancer genome is poorly understood. Here, we demonstrate...
