Search
Search Results
-
Rare antibody phage isolation and discrimination (RAPID) biopanning enables identification of high-affinity antibodies against challenging targets
In vitro biopanning platforms using synthetic phage display antibody libraries have enabled the identification of antibodies against antigens that...
-
Targeting acute myeloid cell surface using a recombinant antibody isolated from whole-cell biopanning of a phage display human scFv antibody library
To discover new therapeutic antibodies for treatment of acute myeloid leukemia (AML) without the requirement of a known antigen, a human single-chain...
-
Streamlined Data Analysis Pipeline for Deep Sequence-Coupled Biopanning Identification of Pathogen-Specific Antibody Responses in Serum
Deep sequence-coupled biopanning (DSCB) is a powerful tool that couples affinity selection of a bacteriophage MS2 virus-like particle peptide display...
-
Streptavidin-Coated Solid-Phase Extraction (SPE) Tips for Antibody Phage Display Biopanning
Phage display is a technique that allows the presentation of unique proteins on the surface of bacteriophages. The phage particles are usually...
-
Antibody Library Screening Using Yeast Biopanning and Fluorescence-Activated Cell Sorting
Yeast surface display (YSD) emerged as a prominent screening methodology for the isolation of monoclonal antibodies (mAbs) against various antigens....
-
Protocol for Differential Biopanning of P. falciparum Phage Display cDNA Library to Identify Parasite Targets of Protective Antibodies
Malaria remains a significant global health burden, killing hundreds of thousands of children annually (WHO, The world malaria report. WHO, Geneva,...
-
Characterization of novel CD19-specific VHHs isolated from a camelid immune library by phage display
BackgroundMonoclonal antibody (mAb)-based immunotherapies have achieved promising outcomes in the treatment of immunological and oncological...
-
Generation of a Naïve Human scFv Phage Display Library and Panning Selection
Phage display antibody libraries have been successfully used as the essential tool to produce monoclonal antibodiesMonoclonal antibodies (mAB)...
-
Antibody Isolation from Human Synthetic Libraries of Single-Chain Antibodies and Analysis Using NGS
Antibody libraries came into existence 30 years ago when the accumulating sequence data of immunoglobulin genes and the advent of PCR technology made...
-
Development and characterization of a novel nanobody with SRMV neutralizing activity
Peste des petits ruminants (PPR) is an acute, contact infectious disease caused by the small ruminant morbillivirus (SRMV), and its morbidity in...
-
Screening of novel peptides that specifically interact with vitamin D bound biocomplex proteins
The majority of the vitamin D that is present in the blood binds to vitamin D binding protein (VDBP) and circulates in the form of a complex...
-
New Tools for Streamlined In Vivo Homing Peptide Identification
In vivo peptide-phage display is an unbiased technique for map** of the vascular diversity and identification of homing peptides. This chapter is...
-
Efficient generation of a stable CHO-K1 cell line overexpressing the human water channel aquaporin-5 as tool to generate therapeutic antibodies
Aquaporins (AQPs) are a family of water permeable channels expressed on the plasma membrane with AQP5 being the major channel expressed in several...
-
Phage Display as a Medium for Target Therapy Based Drug Discovery, Review and Update
Phage libraries are now amongst the most prominent approaches for the identification of high-affinity antibodies/peptides from billions of displayed...
-
Next-Generation Phage Display to Identify Peptide Ligands of Deubiquitinases
Phage display (PD) is a powerful method and has been extensively used to generate monoclonal antibodies and identify epitopes, mimotopes, and protein...
-
Development of an ostrich-derived single-chain variable fragment (scFv) against PTPRN extracellular domain
In type 1 diabetes, the immune system destroys pancreatic beta cells in an autoimmune condition. To overcome this disease, a specific monoclonal...
-
The next generation of biopanning: next gen sequencing improves analysis of bacterial display libraries
BackgroundBacterial surface display libraries are a popular tool for novel ligand discovery due to their ease of manipulation and rapid growth rates....
-
Protocols for Screening Peptides Binding to CCN Family Proteins and Their Extended Utility
The function of CCN family proteins is determined by their interactions with multiple cofactors that are present in the microenvironment. Therefore,...
-
Magnetic Nanoparticle-Based Semi-automated Panning for High-Throughput Antibody Selection
Bio-panning is a common process involved in recombinant antibody selection against defined targets. The biopanning process aims to isolate specific...
-
The rapid and highly parallel identification of antibodies with defined biological activities by SLISY
The therapeutic applications of antibodies are manifold and the emergence of SARS-CoV-2 provides a cogent example of the value of rapidly identifying...
