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Polysaccharide utilization loci-driven enzyme discovery reveals BD-FAE: a bifunctional feruloyl and acetyl xylan esterase active on complex natural xylans
BackgroundNowadays there is a strong trend towards a circular economy using lignocellulosic biowaste for the production of biofuels and other...
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Hemicellulases and Auxiliary Activities for Biomass Hydrolysis
Hemicelluloses are heteropolymers present along with cellulose in all terrestrial plant cell walls. Lignocellulosic biomass consists of 20–30%... -
Characterization of glycoside hydrolase family 11 xylanase from Streptomyces sp. strain J103; its synergetic effect with acetyl xylan esterase and enhancement of enzymatic hydrolysis of lignocellulosic biomass
BackgroundXylanase-containing enzyme cocktails are used on an industrial scale to convert xylan into value-added products, as they hydrolyse the...
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Intracellular removal of acetyl, feruloyl and p-coumaroyl decorations on arabinoxylo-oligosaccharides imported from lignocellulosic biomass degradation by Ruminiclostridium cellulolyticum
BackgroundXylans are polysaccharides that are naturally abundant in agricultural by-products, such as cereal brans and straws. Microbial degradation...
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Xylan-breakdown apparatus of Clostridium thermocellum
Clostridium thermocellum has been studied as a model organism that is highly specialized in cellulose deconstruction, and in secretion of...
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Transcriptome-wide analysis of a superior xylan degrading isolate Penicillium oxalicum 5–18 revealed active lignocellulosic degrading genes
Lignocellulose biomass raw materials have a high value in energy conversion. Recently, there has been growing interest in using microorganisms to...
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An archaeal lid-containing feruloyl esterase degrades polyethylene terephthalate
Polyethylene terephthalate (PET) is a commodity polymer known to globally contaminate marine and terrestrial environments. Today, around 80 bacterial...
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Methionine inducing carbohydrate esterase secretion of Trichoderma harzianum enhances the accessibility of substrate glycosidic bonds
BackgroundThe conversion of plant biomass into biochemicals is a promising way to alleviate energy shortage, which depends on efficient microbial...
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Control of phenotypic diversification based on serial cultivations on different carbon sources leads to improved bacterial xylanase production
Thermobacillus xylanilyticus is a thermophilic and hemicellulolytic bacterium of interest for the production of thermostable hemicellulases. Enzymes’...
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Three marine species of the genus Fulvivirga, rich sources of carbohydrate-active enzymes degrading alginate, chitin, laminarin, starch, and xylan
Bacteroidota is a group of marine polysaccharide degraders, which play a crucial role in the carbon cycle in the marine ecosystems. In this study,...
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Comparative genomic and transcriptome analysis of Bacillus velezensis CL-4 fermented corn germ meal
Bacillus, an excellent organic-degrading agent, can degrade lignocellulose. Notably, some B. velezensis strains encode lignocellulases. However,...
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Optimization and Kinetic Evaluation of Acetylxylan Esterase and Xylanase Production by Trichoderma reesei Using Corn Cob Xylan
AbstractPerformance of Trichoderma reesei in the production of acetylxylan esterase (AXE) and xylanase (Xyl(using corn cob xylan as the growth...
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Characterization of an acetyl xylan esterase from the marine bacterium Ochrovirga pacifica and its synergism with xylanase on beechwood xylan
BackgroundAcetyl xylan esterase plays an important role in the complete enzymatic hydrolysis of lignocellulosic materials. It hydrolyzes the ester...
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Sustainable bioethanol production from enzymatically hydrolyzed second-generation Posidonia oceanica waste using stable Microbacterium metallidurans carbohydrate-active enzymes as biocatalysts
The plant cell wall degradation by extremophilic bacteria is an interesting biological process that is highly relevant for biofuel generation from...
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Feruloyl esterase (FAE-1) sourced from a termite hindgut and GH10 xylanases synergy improves degradation of arabinoxylan
Cereal feedstocks have high arabinoxylan content as their main hemicellulose, which is linked to lignin by hydroxycinnamic acids such as ferulic...
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Identification of a Coprinellus strain and its application in Eucommia ulmoides gum extraction by fermenting leaves
White rot fungi is a kind of filamentous fungi which can degrade lignin, hemicellulose and cellulose effectively. In this study, a wild white rot...
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Towards an understanding of the enzymatic degradation of complex plant mannan structures
Plant cell walls are composed of a heterogeneous mixture of polysaccharides that require several different enzymes to degrade. These enzymes are...
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Characterization of a new bifunctional endo-1,4-β-xylanase/esterase found in the rumen metagenome
Metagenomic data mining of the Nellore cattle rumen microbiota identified a new bifunctional enzyme, endo-1,4-β-xylanase/esterase, which was...
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Xylanase as a Promising Biocatalyst: A Review on Its Production, Purification and Biotechnological Applications
Xylanases are hydrolases that depolymerize the second most abundant polysaccharide in plant cells xylan. The heterogeneous nature of xylan restricts...
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Evaluation of Consolidated Bioprocessing of Sugarcane Biomass by a Multiple Hydrolytic Enzyme Producer Saccharomyces Yeast
Overcoming key bottlenecks for 2G ethanol production, such as the most suitable biomass pretreatment, process layout, and appropriate microorganism,...