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High-Throughput Extraction and Enzymatic Determination of Sugars and Fructans in Fructan-Accumulating Plants
Fructans are carbohydrates present in more than 15% of flowering plants. They represent the major pool of carbohydrates in some species, especially... -
Covalent immobilization of β-galactosidase using a novel carrier alginate/tea waste: statistical optimization of beads modification and reusability
β-galactosidase has been immobilized onto novel alginate/tea waste gel beads (Alg/TW) via covalent binding. Alg/TW beads were subjected to chemical...
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Co-expression of Exo-inulinase and Endo-inulinase Genes in the Oleaginous Yeast Yarrowia lipolytica for Efficient Single Cell Oil Production from Inulin
Yarrowia lipolytica is a promising platform for the single cell oil (SCO) production. In this study, a transformant X+N8 in which exo- and...
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A Comparative Analysis of the Properties of Recombinant Endoinulinase, Exoinulinase, Sucrase, and Alpha-Galactosidase C
As a result of cloning of the inuA , inu1 , aglC , and fopA genes encoding endoinulinase (endoINU), exoinulinase (exoINU), α-galactosidase C (AGLС) and...
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Inulinase: An Important Microbial Enzyme in Food Industry
Inulinases are industrial food enzymes which have earned vast attention recently. Inulin and inulin-containing materials are sustainable, economical... -
Fructooligosaccharides Production from Inulin by Immobilized Endoinulinase on 3-Aminopropyltriethoxysilane Functionalized Halloysite Nanoclay
In the current research, halloysite nanoclay was functionalized by 3-aminopropyltriethoxysilane to immobilize endoinulinase for the preparation of...
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Improving poly-(γ-glutamic acid) production from a glutamic acid-independent strain from inulin substrate by consolidated bioprocessing
To excavate the application of Jerusalem artichoke on poly(γ-glutamic acid) (γ-PGA) production, a γ-PGA producing strain Bacillus amyloliquefaciens ...
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Systematic identification of CAZymes and transcription factors in the hypercellulolytic fungus Penicillium funiculosum NCIM1228 involved in lignocellulosic biomass degradation
BackgroundPenicillium funiculosum NCIM1228 is a filamentous fungus that was identified in our laboratory to have high cellulolytic activity....
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Enhanced Inulin Saccharification by Self-Produced Inulinase from a Newly Isolated Penicillium sp. and its Application in d-Lactic Acid Production
In order to find an alternative for commercial inulinase, a strain XL01 identified as Penicillium sp. was screened for inulinase production. The...
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Annotating Multiple Prebiotic Synthesizing Capabilities Through Whole Genome Sequencing of Fusarium Strain HFK-74
In the present study, seven fungal isolates from effluent treatment plants were screened for the production of prebiotic fructooligosaccharide...
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Overexpression of the Endo-inulinase Gene from Two Different Sources and Characteristics Analysis
The endo-inulinase genes from Aspergillus niger (A. niger) TCCC41064 and Penicillium sp. TN-88 were inserted into the expression vector pPIC9 K. The... -
Novel fold of rotavirus glycan-binding domain predicted by AlphaFold2 and determined by X-ray crystallography
The VP8* domain of spike protein VP4 in group A and C rotaviruses, which cause epidemic gastroenteritis in children, exhibits a conserved...
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Aptitude of endophytic microbes for production of novel biocontrol agents and industrial enzymes towards agro-industrial sustainability
BackgroundEndophytes have continued to receive increased attention worldwide, probably, due to the enormous biotechnological potentials spanning...
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2,3-Butanediol
2,3-Butanediol (2,3-BD) is a fascinating chemical with a wide range of uses. The industrial development of this chemical has not been adequately... -
Exploring the potential of Aspergillus wentii: secondary metabolites and biological properties
Fungi are of considerable importance due to their capacity to biosynthesize various secondary metabolites with bioactive properties that draw high...
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Purification and Characterization of Exo-Inulinase from Paenibacillus sp. d9 Strain
This study intended to purify and characterise exo-inulinase of diesel-degrading Paenibacillus sp. D9. The whole genome sequencing of Paenibacillus ...
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Characterization of phytochemicals from twisted-leaf garlic (Allium obliquum L.) using liquid chromatography coupled with electrospray ionization quadrupole time-of-flight mass spectrometry
IntroductionTwisted-leaf garlic ( Allium obliquum L.) is a wild Allium species, which is traditionally used as aroma plant for culinary purposes due...
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Production of Ethanol from Plant Biomass
The demand for bioethanol has been steadily growing over fossil fuels due to lower greenhouse gas emissions, improved energy security, and rural... -
The Application of Chemometric Methods in the Production of Enzymes Through Solid State Fermentation Uses the Artificial Neural Network—a Review
In the last decade, different multivariate statistical techniques have been applied to assist enzymatic production by microorganisms through solid...
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Actinomyces ruminis sp. nov., an obligately anaerobic bacterium isolated from the rumen of cattle
An obligately anaerobic, rod-shaped, Gram-stain-positive, non-spore-forming, non-motile bacterial strain; designated as CtC72 T was isolated from the...