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Identification of Brain ECM Binding Variable Lymphocyte Receptors Using Yeast Surface Display
Extracellular matrix (ECM) is a rich mixture of proteins and glycans secreted by cells. This includes typical ECM structures such as collagen and...
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Generation of a novel antibody against BxPrx, a diagnostic marker of pine wilt disease
BackgroundBursaphelenchus xylophilus is a pathogenic nematode that causes pine wilt disease (PWD). To prevent the rapid spread of this pathogen,...
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Transient expression of anti-HrpE scFv antibody reduces the hypersensitive response in non-host plant against bacterial phytopathogen Xanthomonas citri subsp. citri
Citrus canker is a bacterial disease caused by Xanthomonas citri subsp. citri ( Xcc ) that affects the citrus industry worldwide. Hrp pili subunits...
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HSP60 mimetic peptides from Mycobacterium leprae as new antigens for immunodiagnosis of Leprosy
The early diagnosis of leprosy serves as an important tool to reduce the incidence of this disease in the world. Phage display (PD) technology can be...
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Selection of Affibody Affinity Proteins from Phagemid Libraries
Herein, we describe a general protocol for the selection of target-binding affinity protein molecules from a phagemid-encoded library. The protocol...
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Quantitative Label-free Determination of Thrombin Using a Chemically-modified M13 Virus-electrode Interface
The use of affinity peptide displaying on the surface of M13 phage has emerged as a promising alternative to antibody-based detection methods owing...
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Selection and identification of a specific peptide binding to ovarian cancer cells from a phage-displayed peptide library
ObjectivesOvarian cancer is one of the most fatal gynecological malignancies. It is emergently needed to select a novel molecular fragment as a...
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CD47Binder: Identify CD47 Binding Peptides by Combining Next-Generation Phage Display Data and Multiple Peptide Descriptors
CD47/SIRPα pathway is a new breakthrough in the field of tumor immunity after PD-1/PD-L1. While current monoclonal antibody therapies targeting...
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A novel human single-domain antibody-drug conjugate targeting CEACAM5 exhibits potent in vitro and in vivo antitumor activity
Leveraging the specificity of antibody to deliver cytotoxic agent into tumor, antibody-drug conjugates (ADCs) have become one of the hotspots in the...
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Eosinophilic esophagitis auxiliary diagnosis based on a peptide ligand to eosinophil cationic protein in esophageal mucus of pediatric patients
Eosinophilic esophagitis (EoE) is a chronic inflammatory condition of the esophagus characterized by increased number of eosinophils. Currently, EoE...
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Detecting disease associated biomarkers by luminescence modulating phages
Assessment of risk for a given disease and the diagnosis of diseases is often based on assays detecting biomarkers. Antibody-based biomarker-assays...
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TUPDB: Target-Unrelated Peptide Data Bank
The isolation of target-unrelated peptides (TUPs) through biopanning remains as a major problem of phage display selection experiments. These TUPs do...
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Probing Vasculature by In Vivo Phage Display for Target Organ-Specific Delivery in Regenerative Medicine
Different mammalian tissues in their normal and pathological state have distinct molecular features in the luminal side of the vessels. These...
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Short Read-Length Next Generation DNA Sequencing of Antibody CDR Combinations from Phage Selection Outputs
Phage display is commonly used to select target-binding antibody fragments from large libraries containing billions of unique antibody clones. In...
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Huc-MSC-derived exosomes modified with the targeting peptide of aHSCs for liver fibrosis therapy
BackgroundEffective therapeutics to stop or reverse liver fibrosis have not emerged, because these potential agents cannot specifically target...
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Assessing Antibody Specificity in Human Serum Using Deep Sequence-Coupled Biopanning
Affinity selection using phage-display technologies is a powerful tool for identifying the peptide epitopes of monoclonal antibodies. Coupling...
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Autoantibodies against cytoskeletons and lysosomal trafficking discriminate sarcoidosis from healthy controls, tuberculosis and lung cancers
Sarcoidosis is a systemic granulomatous disease of unknown etiology. Hypergammaglobulinemia and the presence of autoantibodies in sarcoidosis suggest...
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Map** Polyclonal Antibody Responses to Infection Using Next-Generation Phage Display
Peptide phage display has historically been used to epitope map monoclonal antibodies. More recently, by coupling this method with next-generation...
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A Simple Whole-Plasmid PCR Method to Construct High-Diversity Synthetic Phage Display Libraries
Phage display technology utilises peptide and antibody libraries with very high diversities to select ligands with specific binding properties. The...
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Screening and Identification of a Specific Binding Peptide to Ovarian Cancer Cells from a Phage-Displayed Peptide Library
To select specific binding peptides for imaging and detection of human ovarian cancer. The phage 12-mer peptide library was used to select specific...
