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Deconvolution Microscopy
Since its introduction in 1983, deconvolution microscopy has become a key image-processing tool for visualizing the cellular structures of fixed and...
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Spectral Imaging and Linear Unmixing in Light Microscopy
Fluorescence microscopy is an essential tool for modern biological research. The wide range of available fluorophores and labeling techniques allows...
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Diphtheria toxin, diphtheria-related fusion protein toxins, and the molecular mechanism of their action against eukaryotic cells
Diphtheria toxin remains one of the most successfully studied of the bacterial protein toxins. A detailed understanding of the structure function...
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Model-based Inference of Gene Expression Dynamics from Sequence Information
A dynamic model of prokaryotic gene expression is developed that makes considerable use of gene sequence information. The main contribution arises...
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Plant Cells: Secondary Metabolite Heterogeneity and Its Manipulation
This chapter proposes the concept of rational manipulation of secondary metabolite heterogeneity in plant cell cultures. The heterogeneity of...
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Bioprocess Monitoring Using Near-Infrared Spectroscopy
Near-infrared spectroscopy (NIR) is a nondestructive analytical technique that has been used for simultaneous prediction of the concentrations of...
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Metabolic Flux Analysis Based on 13C-Labeling Experiments and Integration of the Information with Gene and Protein Expression Patterns
The recent progress on metabolic systems engineering was reviewed, in particular focusing on the metabolic flux analysis (MFA) based on the...
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Biochemical Reaction Engineering and Process Development in Anaerobic Wastewater Treatment
Developments in production technology have frequently resulted in the concentrated local accumulation of highly organic-laden wastewaters. Anaerobic...
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Vertebrate Limb Regeneration
In this chapter, we have touched upon some of the key processes of vertebrate limb regeneration from the formation of the wound epithelium to pattern...
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Regenerative Capacity and the Develo** Immune System
Many components of the vertebrate immune system have evolved with dual, interrelated functions of both protecting injured tissues from infection and...
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Total Internal Reflection Fluorescence Microscopy in Single Molecule Nanobioscience
Recent development in total internal reflection fluorescence microscopy (TIRFM) has made it possible to directly monitor the behaviors of...
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Fluorescence Lifetime Imaging Microscopy (FLIM)
Fluorescence lifetime imaging microscopy (FLIM) is a technique to map the spatial distribution of nanosecond excited state lifetimes within...
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Taxonomy and phylogenetic diversity among the yeasts
Yeasts are among the economically and scientifically most important eukaryotic microorganisms known. At present, there are 1,500 recognized species,...
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Genome evolution: Lessons from Genolevures
In the past years, yeast genome-sequencing programs have been widely developed. Two of them, namely Genolevures I and II, were devoted to the...
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Molecular Components of Physiological Stress Responses in Escherichia coli
In order to survive under and adapt to different conditions Escherichia coli has evolved elaborate systems that are able to sense and respond to...
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Applying Metabolic Profiling Techniques for Stimulus-Response Experiments: Chances and Pitfalls
So far it is mainly transcriptome and proteome analysis that has been applied to elucidate the correlation between genotype and phenotype although...
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Regeneration of Articular Cartilage
Loss of articular cartilage from the ends of bones forming diarthrodial joints can be the source of profound pain and disability, and eventually lead...
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Higher Harmonic Generation Microscopy
Higher harmonic-generation, including second harmonic generation and third harmonic generation, leaves no energy deposition to the interacted matters...
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Tracking Movement in Cell Biology
This article is an overview of techniques for measuring movement of proteins, vesicles and cells using digital image processing. Diverse techniques...
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Metabolic Engineering
Metabolic engineering is a powerful methodology aimed at intelligently designing new biological pathways, systems, and ultimately phenotypes...