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Impedimetric Detection of DNA Hybridization: Towards Near-Patient DNA Diagnostics
Immobilization strategies for the attachment of nucleotide probes to both microarrays and microfabricated interdigitated electrodes differ to... -
Immobilization of DNA on Microarrays
Microarrays are new analytical devices that allow the parallel and simultaneous detection of thousands of target compounds. Microarrays, also... -
Immobilization of Nucleic Acids Using Biotin-Strept(avidin) Systems
There are several advantages for using biotin-streptavidin/avidin (strept(avi-din)) systems to immobilize nucleic acids and other molecules. These... -
Immobilization of Oligonucleotides for Biochemical Sensing by Self-Assembled Monolayers: Thiol-Organic Bonding on Gold and Silanization on Silica Surfaces
The covalent immobilization of biomolecules on surfaces is of great interest for many medical and bioanalytical applications. Currently, there is... -
Analytical Ultracentrifuge for the Characterization of Detergent in Solution
We have characterized the hydrodynamic behaviour in H 2 O and D2O of two detergents, dodecyl-β-D-maltoside... -
Analytical Ultracentrifugation Studies of the Influenza M2 Homotetramerization Equilibrium in Detergent Solutions
Though much progress has been made in the field of membrane protein folding, there is still much to learn about the association of transmembrane... -
Self-Association of Phosphorylase Kinase under Molecular Crowding Conditions
Self-association of phosphorylase kinase (PhK) has been studied using analytical ultracentrifugation and dynamic light scattering under the... -
Molecular Properties and Electrostatic Interactions of Linear Poly(allylamine hydrochloride) Chains
The efficiency of two azoinitiators in the polymerization of allylamine salts in water and organic solvents was studied comparatively.... -
Oligomerization of Hydroperoxide Lyase, a Novel P450 Enzyme in Plants
The oligomeric state of fatty acid hydroperoxide lyase (HPL), of molar mass ∼55 kDa is uncertain and it has been reported as a trimer or tetramer... -
Aggregation of Dibutyl Phthalate Molecules in Decalin Solutions Evidenced by Hydrodynamic and Optical Measurements
Hydrodynamic and optical investigations were performed on the binary dibutyl phthalate (DBP)-decalin liquid mixtures with the DBP content varying... -
Ab initio and Constrained Modeling of Phosphorylase
The joint use of small-angle X-ray scattering (SAXS) and hydrodynamic data permits biologically useful reconstructions of protein structures to be... -
Deconvolution Microscopy
Since its introduction in 1983, deconvolution microscopy has become a key image-processing tool for visualizing the cellular structures of fixed and... -
Laser Application of Polymers
Laser ablation of polymers has been studied with designed materials to evaluate the mechanism of ablation and the role of photochemically active... -
Assessing Nitrogen Carrying Capacity for Blue Hill Bay, Maine: A Management Case History
As net-pen aquaculture in coastal waters grows, regulatory agencies must consider the potential for localized eutrophication. To avoid excessive... -
Probing Hydrogen Bonding in Solids Using Solid State NMR Spectroscopy
Solid state nuclear magnetic resonance (NMR) spectroscopy is a powerful and versatile technique for probing structural and dynamic properties of... -
Determination of the absolute fringe number in Rayleigh interference patterns in systems at sedimentation equilibrium via a newly defined function (kΔ)
Analysis of data from the analytical ultracentrifuge used in sedimentation equilibrium modes calls for knowledge of the absolute (as distinct from... -
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Comparative modeling of giant annelid hemoglobins
The information provided by small-angle X-ray scattering, electron microscopy and hydrodynamics for the giant hemoglobins from different annelid... -
Characterization of bovine serum albumin/chlorogenic acid solution mixtures by analytical ultracentrifugation
The aim of this work was to investigate a possible interaction between bovine serum albumin (BSA) and chlorogenic acid (CGA) in KH2PO4/K2HPO4 buffer... -
The state of association of the Na+-translocating reduced nicotinamide adenine dinucleotide:quinone oxidoreductase in detergent solution – an ultracentrifugation study
Na+:reduced nicotinamide adenine dinucleotide:quinone reductase (Na+ -NQR) is a redox-driven sodium pump found in some bacterial respiratory chains....