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Cholera toxin: mechanisms of entry into host cells
Cholera toxin moves from the plasma membrane to the ER of host cells to cause disease. Trafficking in this pathway depends on toxin binding to...
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The Ustilago maydis killer toxins
Killer toxins are small proteins secreted by a number of fungi that are lethal to susceptible cells (generally fungi of the same or related species)....
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Deconvolution Microscopy
Since its introduction in 1983, deconvolution microscopy has become a key image-processing tool for visualizing the cellular structures of fixed and...
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S. cerevisiae K28 toxin – a secreted virus toxin of the A/B family of protein toxins
Since the initial discovery of toxin-secreting killer strains in the yeast Saccharomyces cerevisiae more than 40 years ago, continuous research on...
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Total Internal Reflection Fluorescence Microscopy in Single Molecule Nanobioscience
Recent development in total internal reflection fluorescence microscopy (TIRFM) has made it possible to directly monitor the behaviors of...
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Tracking Movement in Cell Biology
This article is an overview of techniques for measuring movement of proteins, vesicles and cells using digital image processing. Diverse techniques...
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Taxonomy and phylogenetic diversity among the yeasts
Yeasts are among the economically and scientifically most important eukaryotic microorganisms known. At present, there are 1,500 recognized species,...
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Fluorescence Lifetime Imaging Microscopy (FLIM)
Fluorescence lifetime imaging microscopy (FLIM) is a technique to map the spatial distribution of nanosecond excited state lifetimes within...
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Spectral Imaging and Linear Unmixing in Light Microscopy
Fluorescence microscopy is an essential tool for modern biological research. The wide range of available fluorophores and labeling techniques allows...
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Diphtheria toxin, diphtheria-related fusion protein toxins, and the molecular mechanism of their action against eukaryotic cells
Diphtheria toxin remains one of the most successfully studied of the bacterial protein toxins. A detailed understanding of the structure function...
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Cofactor Regeneration at the Lab Scale
Progress made in lab-scale applications of various coenzyme regeneration systems over the last two decades has mainly focused on the applications of...
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Comparative genomics and gene finding in fungi -- Supplement
Online Supplement to Chapter 1
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Telomeres in fungi
Telomeres are the functional elements concluding and defining each linear chromosome in eukaryotes. They play an essential role in protecting genetic...
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The genome of the filamentous fungus Ashbya gossypii: annotation and evolutionary implications
The 9.2 Mb genome of the filamentous fungus Ashbya gossypii consists of seven chromosomes carrying 4718 protein coding genes, 194 tRNA genes, at...
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Schizosaccharomyces pombe comparative genomics; from sequence to systems
The fission yeast Schizosaccharomyces pombe is becoming increasingly important as a model for the characterization and study of many globally...
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From Stationary to Instationary Metabolic Flux Analysis
Metabolic flux analysis using 13C labeled substrates is an important tool for metabolic engineering. Although it has now been evolving for more than...
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Applying Metabolic Profiling Techniques for Stimulus-Response Experiments: Chances and Pitfalls
So far it is mainly transcriptome and proteome analysis that has been applied to elucidate the correlation between genotype and phenotype although...
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Biochemical Reaction Engineering and Process Development in Anaerobic Wastewater Treatment
Developments in production technology have frequently resulted in the concentrated local accumulation of highly organic-laden wastewaters. Anaerobic...
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Biochemical Engineering Aspects of Expanded Bed Adsorption
The economic feasibility of a biotechnological production process is directly linked to the number and efficiency of the processes required during...
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Parallel Reactor Systems for Bioprocess Development
Controlled parallel bioreactor systems allow fed-batch operation at early stages of process development. The characteristics of shaken bioreactors...