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Development of an ostrich-derived single-chain variable fragment (scFv) against PTPRN extracellular domain
In type 1 diabetes, the immune system destroys pancreatic beta cells in an autoimmune condition. To overcome this disease, a specific monoclonal...
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Development of a single-chain variable antibody fragment against a conserved region of the SARS-CoV-2 spike protein
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has prolonged the duration of the pandemic because of the continuous emergence of new...
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Immunoreactivity of humanized single-chain variable fragment against its functional epitope on domain 1 of CD147
Domain 1 of CD147 participates in matrix metalloproteinase (MMP) production and is a candidate for targeted therapy to prevent cancer invasion and...
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Development of a filter device for the prevention of aquatic bacterial disease using a single-chain variable fragment (scFv)-conjugated affinity silk
Infectious disease is one of the most serious problems in the aquaculture industry for ornamental or edible fish. This study attempted to develop a...
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Expression and purification of a native Thy1-single-chain variable fragment for use in molecular imaging
Molecular imaging using singlechain variable fragments (scFv) of antibodies targeting cancer specific antigens have been considered a non-immunogenic...
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Generation and functional characterization of a single-chain variable fragment (scFv) of the anti-FGF2 3F12E7 monoclonal antibody
Single-chain variable fragments (scFvs) are small-sized artificial constructs composed of the immunoglobulin heavy and light chain variable regions...
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Single-chain dimers from de novo immunoglobulins as robust scaffolds for multiple binding loops
Antibody derivatives have sought to recapitulate the antigen binding properties of antibodies, but with improved biophysical attributes convenient...
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Design of an alternate antibody fragment format that can be produced in the cytoplasm of Escherichia coli
With increased accessibility and tissue penetration, smaller antibody formats such as antibody fragments (Fab) and single chain variable fragments...
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Structural and thermodynamic insights into antibody light chain tetramer formation through 3D domain swap**
Overexpression of antibody light chains in small plasma cell clones can lead to misfolding and aggregation. On the other hand, the formation of...
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T cell receptor β-chain-targeting chimeric antigen receptor T cells against T cell malignancies
The success of chimeric antigen receptor (CAR) T cells in treating B cell malignancies comes at the price of eradicating normal B cells. Even though...
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Crystal structures and molecular dynamics simulations of a humanised antibody fragment at acidic to basic pH
Antibody-fragment (Fab) therapy development has the potential to be accelerated by computational modelling and simulations that predict their target...
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Structural insights into the bi-specific cross-over dual variable antibody architecture by cryo-EM
Multi-specific antibodies (msAbs) are being developed as next generation antibody-based therapeutics. Knowledge of the three-dimensional structures,...
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Use of phase plate cryo-EM reveals conformation diversity of therapeutic IgG with 50 kDa Fab fragment resolved below 6 Å
While cryogenic electron microscopy (cryo-EM) is fruitfully used for harvesting high-resolution structures of sizable macromolecules, its application...
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Machine learning modeling for solubility prediction of recombinant antibody fragment in four different E. coli strains
The solubility of proteins is usually a necessity for their functioning. Recently an emergence of machine learning approaches as trained alternatives...
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Tnpo3 controls splicing of the pre-mRNA encoding the canonical TCR α chain of iNKT cells
Unconventional T cells, such as innate natural killer T cells (iNKT) cells, are an important part of vertebrate immune defences. iNKT recognise...
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Comprehensive genome-wide identification and transferability of chromosome-specific highly variable microsatellite markers from citrus species
Citrus species among the most important and widely consumed fruit in the world due to Vitamin C, essential oil glands, and flavonoids. Highly...
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A new catalytic site functioning in antigen cleavage by H34 catalytic antibody light chain
The cleavage reactions of catalytic antibodies are mediated by a serine protease mechanism involving a catalytic triad composed of His, Ser, and Asp...
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Structure, mechanism and crystallographic fragment screening of the SARS-CoV-2 NSP13 helicase
There is currently a lack of effective drugs to treat people infected with SARS-CoV-2, the cause of the global COVID-19 pandemic. The SARS-CoV-2...
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Develo** a dual VEGF/PDL1 inhibitor based on high-affinity scFv heterodimers as an anti-cancer therapeutic strategy
Cancer progression is enhanced by the interaction of programmed death-ligand 1 (PDL1), which is associated with inhibition of the immune response...
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Massively parallel interrogation of protein fragment secretability using SECRiFY reveals features influencing secretory system transit
While transcriptome- and proteome-wide technologies to assess processes in protein biogenesis are now widely available, we still lack global...
