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DNA mismatch and damage patterns revealed by single-molecule sequencing
Mutations accumulate in the genome of every cell of the body throughout life, causing cancer and other diseases
1 ,2 . Most mutations begin as...
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Enzymatic synthesis and nanopore sequencing of 12-letter supernumerary DNA
The 4-letter DNA alphabet (A, T, G, C) as found in Nature is an elegant, yet non-exhaustive solution to the problem of storage, transfer, and...
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DNA 5-methylcytosine detection and methylation phasing using PacBio circular consensus sequencing
Long single-molecular sequencing technologies, such as PacBio circular consensus sequencing (CCS) and nanopore sequencing, are advantageous in...
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A metagenomic DNA sequencing assay that is robust against environmental DNA contamination
Metagenomic DNA sequencing is a powerful tool to characterize microbial communities but is sensitive to environmental DNA contamination, in...
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Droplet-based bisulfite sequencing for high-throughput profiling of single-cell DNA methylomes
The genome-wide DNA methylation profile, or DNA methylome, is a critical component of the overall epigenomic landscape that modulates gene activities...
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Ab Initio investigation for DNA nucleotide bases sequencing using chiral carbon nanobelts and nanotubes
Understanding the interaction mechanism between DNA nucleotide bases and carbon nanomaterials is an important issue in the field of identifying...
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Single-nucleus DNA sequencing reveals hidden somatic loss-of-heterozygosity in Cerebral Cavernous Malformations
Cerebral Cavernous Malformations (CCMs) are vascular malformations of the central nervous system which can lead to moderate to severe neurological...
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Droplet based whole genome amplification for sequencing minute amounts of purified Mycobacterium tuberculosis DNA
Implementation of whole genome sequencing (WGS) for patient care is hindered by limited Mycobacterium tuberculosis ( Mtb ) in clinical specimens and...
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Application of high-throughput single-nucleus DNA sequencing in pancreatic cancer
Despite insights gained by bulk DNA sequencing of cancer it remains challenging to resolve the admixture of normal and tumor cells, and/or of...
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Analytical evaluation of circulating tumor DNA sequencing assays
In China, circulating tumor DNA analysis is widely used and numerous assays are available. Systematic evaluation to help users make informed...
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Base-resolution UV footprinting by sequencing reveals distinctive damage signatures for DNA-binding proteins
Decades ago, it was shown that proteins binding to DNA can quantitatively alter the formation of DNA damage by UV light. This established the...
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Metagenomic DNA sequencing to quantify Mycobacterium tuberculosis DNA and diagnose tuberculosis
Tuberculosis (TB) remains a significant cause of mortality worldwide. Metagenomic next-generation sequencing has the potential to reveal biomarkers...
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KaScape: a sequencing-based method for global characterization of protein‒DNA binding affinity
It is difficult to exhaustively screen all possible DNA binding sequences for a given transcription factor (TF). Here, we developed the KaScape...
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DNA sequencing at the picogram level to investigate life on Mars and Earth
DNA is an incontrovertible biosignature whose sequencing aids in species identification, genome functionality, and evolutionary relationships. To...
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Simultaneous profiling of histone modifications and DNA methylation via nanopore sequencing
The interplay between histone modifications and DNA methylation drives the establishment and maintenance of the cellular epigenomic landscape, but it...
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Unraveling the whole genome DNA methylation profile of zebrafish kidney marrow by Oxford Nanopore sequencing
Zebrafish is a widely used model organism for investigating human diseases, including hematopoietic disorders. However, a comprehensive methylation...
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Comparison of DNA extraction methods for 16S rRNA gene sequencing in the analysis of the human gut microbiome
The gut microbiome is widely analyzed using high-throughput sequencing, such as 16S rRNA gene amplicon sequencing and shotgun metagenomic sequencing...
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ProTInSeq: transposon insertion tracking by ultra-deep DNA sequencing to identify translated large and small ORFs
Identifying open reading frames (ORFs) being translated is not a trivial task. ProTInSeq is a technique designed to characterize proteomes by...
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Multimodal analysis of cell-free DNA whole-methylome sequencing for cancer detection and localization
Multimodal epigenetic characterization of cell-free DNA (cfDNA) could improve the performance of blood-based early cancer detection. However,...
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Foreign DNA detection in genome-edited potatoes by high-throughput sequencing
Genome editing is a powerful breeding technique that introduces mutations into specific gene sequences in genomes. For genome editing in higher...
