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Evaluation of candidate reference genes stability for gene expression analysis by reverse transcription qPCR in Clostridium perfringens
Identification of stable reference genes for normalization purposes is necessary for obtaining reliable and accurate results of reverse transcription...
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Evaluation of candidate reference genes for quantitative real-time PCR normalization in blood from red deer develo** antlers
Sexual selection favors male traits that increase their ability to monopolize the breeding access to several females. Deer antlers are cranial...
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Reference genes to study the sex-biased expression of genes regulating Drosophila metabolism
Sex is an important variable in biology. Notable differences have been observed between male and female Drosophila in regulation of metabolism, in...
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Evaluation and validation of suitable reference genes for quantitative real-time PCR analysis in lotus (Nelumbo nucifera Gaertn.)
The qRT-PCR technique has been regarded as an important tool for assessing gene expression diversity. Selection of appropriate reference genes is...
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Screening and validation of the optimal panel of reference genes in colonic epithelium and relative cancer cell lines
Real-time quantitative polymerase chain reaction (RT-qPCR) is the most common method to determine mRNA expression, and Minimum Information for...
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Selection and validation of reference genes suitable for gene expression analysis by Reverse Transcription Quantitative real-time PCR in Acinetobacter baumannii
Acinetobacter baumannii is a Gram-negative bacterium considered an emerging multi-drug-resistant pathogen. Furthermore, this bacterium can survive in...
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Selection of suitable reference genes for gene expression studies in HMC3 cell line by quantitative real-time RT-PCR
Microglia represent the primary immune defense system within the central nervous system and play a role in the inflammatory processes occurring in...
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Screening and validation of optimal miRNA reference genes in different develo** stages and tissues of Lilium henryi Baker
Dynamic miRNA detection using the qRT-PCR technique requires appropriate reference genes to ensure data reliability. Previous studies have screened...
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Stability evaluation and validation of appropriate reference genes for real-time PCR expression analysis of immune genes in the rohu (Labeo rohita) skin following argulosis
Argulosis is one of the most unrestrained economically significant freshwater fish ectoparasitic diseases. Proper selection or normalization of the...
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Identification of candidate single-nucleotide polymorphisms (SNPs) and genes associated with sugarcane leaf scald disease
Leaf scald, caused by Xanthomonas albilineans , is a severe disease affecting sugarcane worldwide. One of the most practical ways to control it is by...
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Comparative analysis of infected cassava root transcriptomics reveals candidate genes for root rot disease resistance
Cassava root-rot incited by soil-borne pathogens is one of the major diseases that reduces root yield. Although the use of resistant cultivars is the...
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Reference genes for the develo** mouse lung under consideration of biological, technical and experimental confounders
For gene expression analysis, the raw data obtained from RT-qPCR are preferably normalized to reference genes, which should be constantly expressed...
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Evaluation of potential reference genes in the biting midge Culicoides sonorensis for real-time quantitative PCR analyses
Studies examining differentially expressed genes and gene silencing by RNA interference (RNAi) require a set of stably expressed reference genes for...
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Candidate genes under selection in song sparrows co-vary with climate and body mass in support of Bergmann’s Rule
Ecogeographic rules denote spatial patterns in phenotype and environment that may reflect local adaptation as well as a species’ capacity to adapt to...
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Reliable reference genes and abiotic stress marker genes in Klebsormidium nitens
Microalgae have recently emerged as a key research topic, especially as biological models. Among them, the green alga Klebsormidium nitens, thanks to...
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MarFERReT, an open-source, version-controlled reference library of marine microbial eukaryote functional genes
Metatranscriptomics generates large volumes of sequence data about transcribed genes in natural environments. Taxonomic annotation of these datasets...
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Integrative genomic analyses identify candidate causal genes for calcific aortic valve stenosis involving tissue-specific regulation
There is currently no medical therapy to prevent calcific aortic valve stenosis (CAVS). Multi-omics approaches could lead to the identification of...
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Screening and validation of reference genes in Dracaena cochinchinensis using quantitative real-time PCR
Dragon's blood, the red resin derived from the wounded Dracaena , is a precious traditional medicine used by different culture. Dracaena cochinchinensis ...
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Screening of reference genes for microRNA analysis in the study of solider caste differentiation of Formosan subterranean termite Coptotermes formosanus Shiraki
The soldier caste differentiation is a complex process that is governed by the transcriptional regulation and post-transcriptional regulation....
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Identification of RT-qPCR reference genes suitable for gene function studies in the pitaya canker disease pathogen Neoscytalidium dimidiatum
Neoscytalidium dimidiatum is the main causal agent of pitaya canker. Most studies of virulence and pathogenicity genes have measured expression...
