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Uniform [13C,15N]-labeled and glycosylated IgG1 Fc expressed in Saccharomyces cerevisiae
Despite the prevalence and importance of glycoproteins in human biology, methods for isotope labeling suffer significant limitations. Common...
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Metabolic15N labeling of the N-glycosylated immunoglobulin G1 Fc with an engineered Saccharomyces cerevisiae strain
The predominant protein expression host for NMR spectroscopy is Escherichia coli , however, it does not synthesize appropriate post-translation...
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Real-time biosensing of growth hormone on porous silicon by reflectometric interference Fourier transform spectroscopy
Deficiency in human growth hormone (hGH) can have a wide range of effects throughout the body. Early detection of this deficiency improves...
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A comprehensive assessment of selective amino acid 15N-labeling in human embryonic kidney 293 cells for NMR spectroscopy
A large proportion of human proteins contain post-translational modifications that cannot be synthesized by prokaryotes. Thus, mammalian expression...
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Nanomaterials in Animal Nutrition and Disease Treatment: Recent Developments and Future Aspects
Raise in the human population requires increased demand for animal-derived food products. The quality of animal-derived food depends on the...
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Backbone and methyl side-chain resonance assignments of the Fab fragment of adalimumab
Adalimumab is a therapeutic monoclonal antibody developed to target human TNF an important mediator of immune-mediated inflammatory diseases such as...
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Backbone and methyl side-chain resonance assignments of the single chain Fab fragment of trastuzumab
Trastuzumab is a therapeutic monoclonal antibody developed to target human epidermal growth factor receptor 2 (HER2) present at higher levels in...
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Glutamine-free mammalian expression of recombinant glycoproteins with uniform isotope labeling: an application for NMR analysis of pharmaceutically relevant Fc glycoforms of human immunoglobulin G1
Mammalian cells are widely used for producing recombinant glycoproteins of pharmaceutical interest. However, a major drawback of using mammalian...
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Backbone and side-chain resonance assignments of the NISTmAb-scFv and antigen-binding study
Monoclonal antibodies (mAbs) therapeutics are the largest and fastest growing class of biologic drugs, amongst which, the vast majority are...
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Backbone NMR assignment of the yeast expressed Fab fragment of the NISTmAb reference antibody
The monoclonal antibody (mAb) protein class has become a primary therapeutic platform for the production of new life saving drug products. MAbs are...
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Proliferative Activity of Ehrlich Carcinoma Cells After Use of Nanocomplexes
Investigation of functional state of tumor cells, peculiarities of the pathology development, and sensitivity of tumor cells to the therapeutic...
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Top-down proteomics
Proteoforms, which arise from post-translational modifications, genetic polymorphisms and RNA splice variants, play a pivotal role as drivers in...
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Treatment of Cutaneous Lymphomas: Topical, Systemic, and Radiation Therapies
Cutaneous lymphomas are a highly heterogeneous group of B- or T-lymphocyte-derived malignancies; they are generally associated with a relatively...
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Catching the Conformational Wave: Measuring the Working Strokes of Protofilaments as They Curl Outward from Disassembling Microtubule Tips
Optical traps have enabled foundational studies of how mechanoenzymes such as kinesins and dynein motors walk along microtubules, how myosins move...
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NMR assignments of the N-glycans of the Fc fragment of mouse immunoglobulin G2b glycoprotein
The Fc portion of immunoglobulin G (IgG) promotes defensive effector functions in the immune system by interacting with Fcγ receptors and complement...
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Leveraging Advancement in Robotics in the Treatment of Cancer
The limits of lack of specificity, short circulation half-lives as well as limited bioavailability and solubility have necessitated the application...
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Fluid interfacial energy drives the emergence of three-dimensional periodic structures in micropillar scaffolds
Structures that are periodic on a microscale in three dimensions are abundant in nature, for example, in the cellular arrays that make up living...
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A Study of the Macrophage Differentiation of Acute Myeloid Leukemia Cells in Multicellular Aggregates
Abstract —The resistance of leukemic cells to the action of chemotherapeutic drugs is the main reason for insufficient efficacy in the treatment of...
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Metal–organic frameworks for biological applications
Metal–organic frameworks (MOFs) have emerged as exciting potential new candidates for application across nanomedicine, with the first example now in...
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NMR map** of the highly flexible regions of 13C/15N-labeled antibody TTAC-0001-Fab
Monoclonal antibody (mAb) drugs are clinically important for the treatment of various diseases. TTAC-0001 is under development as a new anti-cancer...
