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Regulatory Issues for Process Development and Manufacture of Plasmids Under Contract
By now, almost anyone involved in the biological sciences has gone through the process of purifying plasmid DNA from a bacterial host organism to use...
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Validation of Gene Therapy Manufacturing Processes
Specific issues of concern in the validation of gene therapy viral vector manufacturing processes include quality of raw materials, safety testing of...
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Liposomes and Phospholipid Binding Proteins in Glycoprotein Biosynthesis
The metabolic process of glycosylation of proteins on the amide nitrogen of specific asparagine residues in proteins, referred to as N-linked...
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Preparation, Isolation, and Characterization of Liposomes Containing Natural and Synthetic Lipids
The specificity, homogeneity, and availability of large-batch production of liposomes with natural lipids and synthetic lipids have made them an...
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Protein Binding Matrices
Historically, the origin of gene therapy, the revolutionary new field of human medicine1, was underlined by development of molecular genetic,...
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Quality Assurance and Quality Control for Viral Therapeutics
It is the job of the Quality Control (QC) function to provide the data that a company uses to assure the public and regulators that its products are...
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Inhibition of heat-induced denaturation of albumin by nonsteroidal antiinflammatory drugs (NSAIDs): Pharmacological implications
The activity of nonsteroidal antiinflammatory drugs (NSAIDs) in rheumatoid arthritis is not only due to the inhibition of the production of...
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Purification of Supercoiled Plasmid
Current technologies for the purification of supercoiled plasmids are limited. The use of cesium chloride gradients in the presence of ethidium...
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Chemical Construction of Immunotoxins
Immunotoxins (ITs) are chimeric proteins consisting of an antibody linked to a toxin. The antibody confers specificity (ability to recognize and...
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Preparation of Recombinant RNase Single-Chain Antibody Fusion Proteins
Selective cytotoxicity is an important goal of specific drug targeting. Toward this end, toxins isolated primarily from higher plants and bacteria...
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Construction of Ribonuclease–Antibody Conjugates for Selective Cytotoxicity
Immunotoxins based on human and humanized ribonuclease may have potential for cancer therapy while exhibiting less toxic side effects and stimulating...
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Liquid chromatography of polysaccharides
This review gives an analysis of methods of high-performance liquid chromatography for the separation of various polysaccharides.
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Synthesis, Formation, and Occurrence of Contaminants in Biotechnologically Manufactured L-Tryptophan
The pattern of contaminants in pharmaceutical and feed grade L-tryptophan (Trp) was investigated in a market survey of 22 lots of 6 different...
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Physicochemical properties of recombinant hepatitis B surface antigen expressed in mammalian cell (C127)
The physicochemical properties of recombinant hepatitis B surface antigen (r-HBsAg), which was expressed in C127 mammalian cell were studied. Using...
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Nitric oxide synthase from bovine pancreas: Purification and characterization
Nitric oxide synthase, NOS (EC.1.14.13.39), was purified from bovine pancreas over 5,500-fold with a 7.6% yield using 30% ammonium sulfate...
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Effect of hepatic cirrhosis on the pharmacokinetics of theophylline in rats
The experimental hepatic cirrhosis was induced either by bile duct ligation (BDL) or by pretreatment with dimethylnitrosamine (DMNA). The...