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Concordance of bone culture and deep tissue culture during the operation of diabetic foot osteomyelitis and clinical characteristics of patients
PurposeTo retrospectively analyze the concordance of bacterial culture between bone tissue and deep soft tissue in diabetic foot osteomyelitis (DFO)...
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Mycobacterial tissue culture positivity in the work-up of suspected cutaneous infection in a rural dermatology patient population
Dermatologists may be over-ordering mycobacterial tissue cultures when performing the broad work-up of skin infection. The objective of this study...
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The challenge of ovarian tissue culture: 2D versus 3D culture
BackgroundCryopreservation of ovarian tissue is a powerful technique for preserving female fertility, as it can restore fertility and endocrine...
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Co-culture of human cryopreserved fragmented ovarian tissue with theca progenitor cells derived from theca stem cells
PurposeDespite the significant advances in the in vitro development of human primordial follicles, it is still a challenging approach with great...
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Culture of equine intestinal epithelial stem cells after delayed tissue storage for future applications
BackgroundEquine intestinal epithelial stem cells (ISCs) serve as potential targets to treat horses with severe intestinal injury. The ability to...
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Evaluation of Dundee and ALT-70 predictive models for cellulitis in 56 patients who underwent tissue culture
Nonpurulent cellulitis lacks a gold standard to distinguish noninfectious sources of inflammation. Two models have been created that evaluate...
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Tissue-engineered anterior segment eye cultures demonstrate hallmarks of conventional organ culture
BackgroundGlaucoma is a blinding disease largely caused by dysregulation of outflow through the trabecular meshwork (TM), resulting in elevated...
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Phyto-factories of anti-cancer compounds: a tissue culture perspective
BackgroundCancer is one of the most critical but ubiquitous causes of death grappled from past decades. Widely used chemotherapy with cytotoxic...
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The value of sonication in the differential diagnosis of septic and aseptic femoral and tibial shaft nonunion in comparison to conventional tissue culture and histopathology: a prospective multicenter clinical study
BackgroundSeptic and aseptic nonunion require different therapeutic strategies. However, differential diagnosis is challenging, as low-grade...
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Successful isolation of viable stem cells from cryopreserved microfragmented human adipose tissue from patients with knee osteoarthritis – a comparative study of isolation by tissue explant culture and enzymatic digestion
PurposeTo investigate if viable stem cells could be isolated and expanded from cryopreserved microfragmented adipose tissue (AT) harvested from...
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The effect of mTOR activation and PTEN inhibition on human primordial follicle activation in ovarian tissue culture
PurposeThe effect of PTEN inhibitor (Bpv(HOpic); Bpv) and mTOR activators (phosphatidic acid (PA) and propranolol (PP)), were evaluated on the...
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In vitro growth (IVG) of human ovarian follicles in frozen thawed ovarian cortex tissue culture supplemented with follicular fluid under hypoxic conditions
BackgroundDespite its clinical success rates, transplantation after ovarian tissue cryopreservation (OTC) remains a matter of concern. Certain cancer...
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Results of a monocentric field study: value of histology compared to sonication method and conventional tissue culture in the diagnosis of periprosthetic joint infection (PJI)
BackgroundTo confirm the diagnosis of periprosthetic joint infection (PJI), the Infectious Diseases Society of America (IDSA) and the International...
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Image-guided percutaneous bone biopsy for pediatric osteomyelitis: correlating MRI findings, tissue pathology and culture, and effect on clinical management
Bone biopsy remains the gold standard for diagnosis of osteomyelitis while MRI results in a radiologic diagnosis that generally precedes biopsy. This...
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Formulation and evaluation of nanobiotherapeutics of Terminalia arjuna through plant tissue culture for atherosclerosis
BackgroundThe study seeks to investigate the therapeutic potential of Terminalia arjuna callus in addressing atherosclerosis. In order to get...
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Efficacy and safety of two-stage revision for patients with culture-negative versus culture-positive periprosthetic joint infection: a single-center retrospective study
BackgroundThe safety and efficacy of two-stage revision for culture-negative PJI remain controversial. This study analyzed outcomes after two-stage...
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Investigating the impact of vitrification on bovine ovarian tissue morphology, follicle survival, and transcriptomic signature
PurposeOvarian tissue cryopreservation is vital for fertility preservation, yet its effect on ovarian tissue follicle survival and transcriptomic...
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Tendon extracellular-matrix-derived tissue engineering micro-tissue for Achilles tendon injury regeneration in rats
BackgroundAchilles tendon is vital in maintaining the stability and function of ankle joint. It is quite difficult to achieve the structural and...
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Concordance between culture, Molecular Culture and Illumina 16S rRNA gene amplicon sequencing of bone and ulcer bed biopsies in people with diabetic foot osteomyelitis
BackgroundIn clinical practice the diagnosis of diabetic foot osteomyelitis (DFO) relies on cultures of bone or ulcer bed (UB) biopsies, of which...
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Thoracic adipose tissue contributes to severe virus infection of the lung
ObjectiveObesity is an independent risk factor for severe influenza virus and COVID-19 infections. There might be an interplay between adipose tissue...