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Detection and quantification of JAK2V617F copy number by droplet digital PCR versus real-time PCR
Myeloproliferative neoplasm (MPN) usually has an adverse prognosis, progressing to acute leukemia or splanchnic vein thromboses (SVTs). Therefore,...
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Rapid ABO genoty** method using loop-mediated isothermal amplification (LAMP) and real-time PCR
Analyzing all biological evidence at a crime scene presents serious time, budget, and labor constraints. Therefore, selecting valid evidence is...
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Establishment of a real-time fluorescent quantitative PCR detection method and phylogenetic analysis of BoAHV-1
BackgroundInfectious bovine rhinotracheitis (IBR), caused by Bovine alphaherpesvirus-1 (BoAHV-1), is an acute, highly contagious disease primarily...
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A cross-sectional study on performance evaluation in Italian standardbred horses’ real-time PCR-positive for Theileria equi
BackgroundInflammatory myopathy and perivasculitis have been recently described in horses with chronic equine piroplasmosis (EP). These alterations...
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Environmental detection of eumycetoma pathogens using multiplex real-time PCR for soil DNA in Sennar State, Sudan
BackgroundMycetoma is a chronic disease affecting the skin and subcutaneous tissue endemic in the tropical and subtropical regions. Several bacteria...
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Evaluation of real-time NASBA assay for the detection of SARS-CoV-2 compared with real-time PCR
PurposeIn January 2020, the COVID-19 pandemic started and has severely affected all countries around the world. The clinical symptoms alone are not...
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Evaluation of a real-time mobile PCR device (PCR 1100) for the detection of the rabies gene in field samples
BackgroundThe Philippines is ranked among the top countries with 200–300 annual deaths due to rabies. Most human rabies cases have been reported in...
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Long-range and real-time PCR identification of a large SERPINC1 deletion in a patient with antithrombin deficiency
Congenital antithrombin (AT) or serpin C1 deficiency, caused by a SERPINC1 abnormality, is a high-risk factor for venous thrombosis. SERPINC1 is...
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Enhanced detection rate of Mycoplasma genitalium in urine overtime by transcription-mediated amplification in comparison to real-time PCR
BackgroundDiagnosis of infected individuals with Mycoplasma genitalium (MG) is often performed by real-time PCR or transcription-mediated...
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Multiplex real-time PCR FilmArray performance in the diagnosis of meningoencephalitis: lights and shadows
PurposeWe aimed to evaluate the performance of the FilmArray (FA) meningitis/encephalitis (ME) panel. Secondarily, we analyzed the false positive...
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Development and validation of multiplex real-time PCR for simultaneous detection of six bacterial pathogens causing lower respiratory tract infections and antimicrobial resistance genes
BackgroundKlebsiella pneumoniae, Acinetobacter baumannii, Pseudomonas aeruginosa , Escherichia coli, Streptococcus pneumoniae and Staphylococcus aureus ...
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Comparison of real-time PCR and nested PCR for toxoplasmosis diagnosis in toxoplasmic retinochoroiditis patients
BackgroundsPCR is a proper technique that significantly improves toxoplasmosis diagnosis. However, a more sensitive technique is required. This study...
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Systematic Review on the Correlation Between SARS-CoV-2 Real-Time PCR Cycle Threshold Values and Epidemiological Trends
BackgroundThe ability to proactively predict the epidemiological dynamics of infectious diseases such as coronavirus disease 2019 (COVID-19) would...
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Genetic diversity of porcine reproductive and respiratory syndrome virus and evaluation of three one-step real-time RT-PCR assays in Korea
BackgroundPorcine reproductive and respiratory syndrome virus (PRRSV) has caused huge economic losses in the global swine industry. Frequent genetic...
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Development and clinical validation of a one-step pentaplex real-time reverse transcription PCR assay for detection of hepatitis virus B, C, E, Treponema pallidum, and a human housekee** gene
BackgroundWith the safety of blood transfusion being a major public health concern, the development of a rapid, sensitive, specific, and...
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Molecular beacon based real-time PCR p1 gene genoty**, macrolide resistance mutation detection and clinical characteristics analysis of Mycoplasma pneumoniae infections in children
BackgroundMycoplasma pneumoniae can be divided into different subtypes on the basis of the sequence differences of adhesive protein P1, but the...
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SMA carrier testing using Real-time PCR as a potential preconception screening tool
BackgroundSMA is a neuromuscular genetic disorder causing irreversible degeneration of the anterior horn cells of lower motor neurons. According to...
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Simultaneous detection and identification of Peste des petits ruminants Virus Lineages II and IV by MCA-Based real-time quantitative RT-PCR assay within single reaction
BackgroundPeste des petits ruminants (PPR) disease is a cross-species infectious disease that severely affects small ruminants and causes great...
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Establish an allele-specific real-time PCR for Leishmania species identification
BackgroundLeishmaniasis is a serious neglected tropical disease that may lead to life-threatening outcome, which species are closely related to...
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Evaluation and comparison of one-step real-time PCR and one-step RT-LAMP methods for detection of SARS-CoV-2
BackgroundThere is an increasing disease trend for SARS-COV-2, so need a quick and affordable diagnostic method. It should be highly accurate and...
