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Diagnostic value of MDM2 RNA in situ hybridization for low-grade osteosarcoma: Consistency comparison of RNA in situ hybridization, fluorescence in situ hybridization, and immunohistochemistry
Detection of MDM2 gene amplification via fluorescence in situ hybridization (FISH) and MDM2 overexpression by immunohistochemistry (IHC) have been...
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Immunoglobulin light chain transcript detection by ultrasensitive RNA in situ hybridization for B-cell lymphoma diagnosis
Evaluation of B-cell clonality can be challenging in the interpretation of lymphoid infiltrates on tissue sections. Clonality testing based on IG ...
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Fluorescent in situ hybridization has limitations in screening NRG1 gene rearrangements
BackgroundNRG1 fusion is a promising therapeutic target for various tumors but its prevalence is extremely low, and there are no standardized testing...
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HPV-Associated Squamous Cell Carcinoma of the Eyelid: Diagnostic Utility of p16 Immunohistochemistry and mRNA In Situ Hybridization
BackgroundHigh-risk (HR) Human papillomavirus (HPV) has been implicated in pathogenesis of squamous cell carcinomas (SCC) at several sites with...
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Clinical application of the HM-1000 image processing for HER2 fluorescence in situ hybridization signal quantification in breast cancer
BackgroundAccurate quantification of human epidermal growth factor receptor 2 ( HER2 ) gene amplification is important for predicting treatment...
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Evaluation of TRIM63 RNA in situ hybridization (RNA-ISH) as a potential biomarker for alveolar soft-part sarcoma (ASPS)
Alveolar soft-part sarcoma (ASPS) is a rare soft tissue tumor with a broad morphologic differential diagnosis. While histology and...
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A Novel Scoring System for MYB RNA In Situ Hybridization Displays High Sensitivity and Specificity for Adenoid Cystic Carcinoma in a Clinical Setting
BackgroundMYB RNA in situ hybridization (ISH) has emerged as a reliable and accessible marker to support adenoid cystic carcinoma (ACC) diagnosis,...
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An in situ hybridization study of syndecan family during the late stages of develo** mouse molar tooth germ
Expression of syndecan - 1, 2, 3 , and 4 mRNAs during the late stages of tooth germ formation was investigated by in situ hybridization, using [ 35 S]-UTP-...
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Diagnosis of Adenoid Cystic Carcinoma with Striking Tubular Hypereosinophilia by MYB and EWSR1 Breakapart Fluorescence In Situ Hybridization
BackgroundAdenoid cystic carcinoma (AdCC), associated with MYB/MYBL1 gene rearrangements, shows epithelial and basaloid myoepithelial cells arranged...
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CDKN2A/B deletion in IDH-mutant astrocytomas: An evaluation by Fluorescence in-situ hybridization
IntroductionCDKN2A/B homozygous deletion is one of the defining features of grade 4 in IDH -mutant astrocytic tumours.
AimTo evaluate CDKN2A/B- deleti...
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HER2 copy number determination in breast cancer using the highly sensitive droplet digital PCR method
Human epidermal growth factor receptor 2 (HER)-positive breast cancer (BC) is characterized by an aggressive clinical course. In the case of HER2...
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A new diagnostic algorithm using biopsy specimens in adult T-cell leukemia/lymphoma: combination of RNA in situ hybridization and quantitative PCR for HTLV-1
Histopathological distinction between adult T-cell leukemia/lymphoma (ATLL) and other T-cell neoplasms is often challenging. The current gold...
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PDGFB RNA in situ hybridization for the diagnosis of dermatofibrosarcoma protuberans
Dermatofibrosarcoma protuberans (DFSP) is a spindle cell neoplasm of the skin and superficial soft tissue with a tendency for locally aggressive...
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Impact of HER2-low status for patients with early-stage breast cancer and non-pCR after neoadjuvant chemotherapy: a National Cancer Database Analysis
PurposeTo investigate potential differences in pathological complete response (pCR) rates and overall survival (OS) between HER2-low and HER2-zero...
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Unusual split green-orange signals in USP6 fluorescence in situ hybridization in a malignant peripheral nerve sheath tumor with a novel NF1-SCIMP fusion: a potential diagnostic pitfall
Deletion of the neurofibromatosis 1 ( NF1 ) gene is common, but NF1 rearrangement or fusion has rarely been reported in peripheral nerve sheath tumors....
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Nodal pCR and overall survival following neoadjuvant chemotherapy for node positive ER+/Her2- breast cancer
PurposeThe role of neoadjuvant chemotherapy (NAC) in node-positive (N+) ER+/HER2- breast cancer (BC) is debated, given low total pathologic complete...
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Variable levels of spike and ORF1ab RNA in post-mortem lung samples of SARS-CoV-2-positive subjects: comparison between ISH and RT-PCR
Post-mortem examination plays a pivotal role in understanding the pathobiology of the SARS-CoV-2; thus, the optimization of virus detection on the...
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Correlating SS18-SSX immunohistochemistry (IHC) with SS18 fluorescent in situ hybridization (FISH) in synovial sarcomas: a study of 36 cases
The recently introduced, highly sensitive and specific SS18-SSX immunohistochemistry (IHC) is an attractive alternative to SS18 fluorescence in situ...
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Accuracy of HPV E6/E7 mRNA examination using in situ hybridization in diagnosing cervical intraepithelial lesions
BackgroundThe consistency of pathologists in the diagnosis of cervical intraepithelial neoplasia (CINs) is not ideal, especially between low- and...
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Clinical significance of quantitative categorization of HER2 fluorescent in situ hybridization results in invasive breast cancer patients treated with HER2-targeted agents
HER2 ( ERBB2 ) gene status serves as a strong predictive marker of response to HER2-targeted agents in invasive breast cancers, albeit with...
