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A duplex nested RT-PCR method for monitoring porcine epidemic diarrhea virus and porcine delta-coronavirus
BackgroundPorcine epidemic diarrhea virus (PEDV) and porcine delta-coronavirus (PDCoV) are economically important pathogens that cause diarrhea in...
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Comparison of real-time PCR and nested PCR for toxoplasmosis diagnosis in toxoplasmic retinochoroiditis patients
BackgroundsPCR is a proper technique that significantly improves toxoplasmosis diagnosis. However, a more sensitive technique is required. This study...
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Development and evaluation of a simple PCR assay and nested PCR for rapid detection of clarithromycin-resistant Helicobacter pylori from culture and directly from the biopsy samples in India
BackgroundEradication of Helicobacter pylori provides the most effective treatment for gastroduodenal diseases caused by H. pylori infection....
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Investigation of Toxoplasma infection in zoo animals using multispecies ELISA and GRA7 nested PCR
BackgroundToxoplasma is an obligate intracellular protozoan that causes an important zoonotic disease with a worldwide distribution. Felids are the...
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Extraction of the DNA of Giardia lamblia isolated from vegetables and fruits in a simplified way and its diagnosis using Nested-PCR
Giardiasis is one of the main diseases transmitted by food contaminated with infectious stages. It was usually spread through freshly eaten food. In...
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Highly sensitive nested polymerase chain reaction to improve the detection of Leishmania species in clinical specimens
Cutaneous leishmaniasis (CL) is one of the most neglected tropical diseases and an important health problem in many countries. It is an endemic...
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Expression of Extracellular Regulated Protein Kinase (ERK) & Promoter Methylation of RASSF1A In Endometrioid Endometrial Carcinoma and Its Precursor Lesions – A Nested Case Control Study and Review of Literature
ObjectiveEndometrial carcinoma (EC) is the most common invasive gynaecological cancer worldwide, majority being endometroid endometrial carcinoma...
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Comparison of bacterial culture with BioFire® FilmArray® multiplex PCR screening of archived cerebrospinal fluid specimens from children with suspected bacterial meningitis in Nigeria
BackgroundDiagnosis of bacterial meningitis remains a challenge in most develo** countries due to low yield from bacterial culture, widespread use...
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Rapid genoty** of F8 intron 22 inversion by nested PCR based on long-distance PCR
F8 intron 22 inversion (Inv22) accounts for about 40% of severe hemophilia A (HA) cases and is mainly genotyped by long-distance PCR (LD-PCR) or...
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Performances of single tube nested polymerase chain reaction and GeneXpert ultra on Formalin fixed paraffin embedded tissues in the diagnosis of tuberculous spondylodiscitis
IntroductionTuberculous Spondylodiscitis is the most common form of musculoskeletal tuberculosis. Molecular techniques on fresh tissues are proved to...
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Development of a new multiplex PCR to detect fecal coccidian parasite
BackgroundCryptosporidium spp . , Cystoisospora belli and Cyclospora cayetanensis are common intestinal coccidian parasites causing gastroenteritis....
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Association of circulating let-7b-5p with major depressive disorder: a nested case-control study
BackgroundMajor depressive disorder (MDD) is one of the most common psychiatric disorders and is a great disease burden. However, its underlying...
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Association between academic pressure, NR3C1 gene methylation, and anxiety symptoms among Chinese adolescents: a nested case-control study
BackgroundAcademic pressure is a prevalent stressor among Chinese adolescents and is often linked to anxiety symptoms, although the underlying...
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The association of FKBP5 gene methylation, adolescents’ sex, and depressive symptoms among Chinese adolescents: a nested case-control study
BackgroundDepressive symptoms among adolescents are a serious health concern around the world. Altered DNA methylation in the FK506 binding protein 5...
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Genetic variants of the GLP-1R gene affect the susceptibility and glucose metabolism of gestational diabetes mellitus: a two-center nested case‒control study
BackgroundGestational diabetes mellitus (GDM) is the most common complication during pregnancy, occurring under the combined action of environmental...
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Longitudinal changes of oxidative stress and PON1 lactonase activity and status in older pregnant women undergoing assisted reproductive technology: a prospective nested case-control study
BackgroundChildbearing in women with advanced maternal age (AMA) has increased the need for artificial reproductive technology (ART). ART and...
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Gut Microbiota Diversity and Overweight/Obesity in Infancy: Results from a Nested Case-control Study
ObjectiveChildhood obesity is a major health concern worldwide. Previous studies have explored the relationship between obesity and gut microbiota....
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Comparison of endotracheal aspirate and bronchoalveolar lavage fluid metagenomic next-generation sequencing in severe pneumonia: a nested, matched case–control study
ObjectivesTo compare clinical outcomes in patients with severe pneumonia according to the diagnostic strategy used.
MethodsIn this retrospective,...
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Epidemiology of epizootic lymphangitis of carthorses in northern Ethiopia using conventional diagnostic methods and nested polymerase chain reaction
BackgroundEpizootic lymphangitis (EL), caused by Histoplasma capsulatum variety farciminosum (HCF) is a contagious, chronic disease of equines,...
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Accurate and affordable detection of rifampicin and isoniazid resistance in Tuberculosis sputum specimens by multiplex PCR-multiple probes melting analysis
BackgroundEscalating cases of multidrug-resistant tuberculosis (MDR-TB) pose a major challenge to global TB control efforts, necessitating innovative...
