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Borrelia multiplex: a bead-based multiplex assay for the simultaneous detection of Borrelia specific IgG/IgM class antibodies
BackgroundLyme borreliosis (LB) is the most common tick-borne infectious disease in the northern hemisphere. The diagnosis of LB is usually made by...
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The clinical impact of multiplex PCR panel diagnostics in paediatric meningitis/ encephalitis: a bicenter cohort study
PurposeIn infections of the Central Nervous System (iCNS), rapid identification of causing pathogens is crucial for survival and to avoid long-term...
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Panel-based RNA fusion sequencing improves diagnostics of pediatric acute myeloid leukemia
New methods like panel-based RNA fusion sequencing (RNA-FS) promise improved diagnostics in various malignancies. We here analyzed the impact of...
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Accurate and affordable detection of rifampicin and isoniazid resistance in Tuberculosis sputum specimens by multiplex PCR-multiple probes melting analysis
BackgroundEscalating cases of multidrug-resistant tuberculosis (MDR-TB) pose a major challenge to global TB control efforts, necessitating innovative...
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ALEX versus ISAC multiplex array in analyzing food allergy in atopic children
ALEX multiplex array is a relatively new multiplex allergy test which analyses more than 120 allergen extracts and 170 molecular components. ISAC is...
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Multiplex real-time PCR FilmArray performance in the diagnosis of meningoencephalitis: lights and shadows
PurposeWe aimed to evaluate the performance of the FilmArray (FA) meningitis/encephalitis (ME) panel. Secondarily, we analyzed the false positive...
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Development and validation of multiplex real-time PCR for simultaneous detection of six bacterial pathogens causing lower respiratory tract infections and antimicrobial resistance genes
BackgroundKlebsiella pneumoniae, Acinetobacter baumannii, Pseudomonas aeruginosa , Escherichia coli, Streptococcus pneumoniae and Staphylococcus aureus ...
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Comparison of bacterial culture with BioFire® FilmArray® multiplex PCR screening of archived cerebrospinal fluid specimens from children with suspected bacterial meningitis in Nigeria
BackgroundDiagnosis of bacterial meningitis remains a challenge in most develo** countries due to low yield from bacterial culture, widespread use...
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Decoding human cytomegalovirus for the development of innovative diagnostics to detect congenital infection
AbstractCytomegalovirus is the most common cause of congenital infectious disease and the leading nongenetic etiology of sensorineural hearing loss....
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How molecular diagnostics help us to correctly identify pet allergies
Simultaneous sensitization to two or more animal species is common and represents a regular diagnostic problem during conventional extract-based...
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Improved Diagnostics in Bacterial Neonatal Meningitis Using a Next-Generation Sequencing Platform
IntroductionBacterial meningitis in infants is an infrequent but life-threatening condition. Empiric therapy should begin as soon as meningitis is...
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Monoplex and multiplex immunoassays: approval, advancements, and alternatives
Immunoassays are a powerful diagnostic tool and are widely used for the quantification of proteins and biomolecules in medical diagnosis and...
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High-multiplex tissue imaging in routine pathology—are we there yet?
High-multiplex tissue imaging (HMTI) approaches comprise several novel immunohistological methods that enable in-depth, spatial single-cell...
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Influenza A, Influenza B, human respiratory syncytial virus and SARSCoV-2 molecular diagnostics and epidemiology in the post COVID-19 era
BackgroundThe concurrent circulation of SARS-CoV-2 with other respiratory viruses is unstoppable and represents a new diagnostic reality for...
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Title-molecular diagnostics of dystrophinopathies in Sri Lanka towards phenotype predictions: an insight from a South Asian resource limited setting
BackgroundThe phenotype of Duchenne muscular dystrophy (DMD) and Becker muscular dystrophy (BMD) patients is determined by the type of DMD gene...
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Microfluidics-based strategies for molecular diagnostics of infectious diseases
Traditional diagnostic strategies for infectious disease detection require benchtop instruments that are inappropriate for point-of-care testing...
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Multiplex PCR for detection of Anaplasma marginale, A. bovis and A. platys in cattle
Anaplasma genus has been classified under the family Anaplasmataceae of order Rickettsiales, which contains the spp. Anaplasma marginale , Anaplasma...
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The Evolving Landscape of Diagnostics for Invasive Fungal Infections in Lung Transplant Recipients
Purpose of ReviewThe objective of this paper is to review the armamentarium of tests available for diagnosis of invasive fungal infections (IFI) in...
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Estimating prognostic relevant cutoff values for a multiplex PCR detecting BCR::ABL1 in chronic myeloid leukemia patients on tyrosine kinase inhibitor therapy in resource-limited settings
The prognosis of chronic myeloid leukemia (CML) on tyrosine kinase inhibitor (TKI) treatment is based on the quantification of BCR::ABL1 fusion gene...
