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Analysis of intestinal flora and cognitive function in maintenance hemodialysis patients using combined 16S ribosome DNA and shotgun metagenome sequencing
BackgroundCognitive impairment is widely prevalent in maintenance hemodialysis (MHD) patients, and seriously affects their quality of life. The...
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Delineation of the bacterial composition in exogenous endophthalmitis using 16S rDNA sequencing
PurposeTo determine the bacterial spectrum of exogenous endophthalmitis of different origins, namely, posttraumatic, postcataract surgery, filtering...
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Concordance between culture, Molecular Culture and Illumina 16S rRNA gene amplicon sequencing of bone and ulcer bed biopsies in people with diabetic foot osteomyelitis
BackgroundIn clinical practice the diagnosis of diabetic foot osteomyelitis (DFO) relies on cultures of bone or ulcer bed (UB) biopsies, of which...
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16S rRNA gene sequencing reveals the effect of fluoxetine on gut microbiota in chronic unpredictable stress-induced depressive-like rats
ObjectivesGut microbiota is relevant to the pathogenesis of mental disorders including depression. This study aimed to investigate the influence of...
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Changes in the fecal microbiota of breast cancer patients based on 16S rRNA gene sequencing: a systematic review and meta-analysis
PurposeBreast cancer (BC) is a devastating disease for women. Microbial influences may be involved in the development and progression of breast...
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Alterations of gut microbiota in infants with biliary atresia identified by 16S rRNA-sequencing
BackgroundBiliary atresia (BA) is a severe neonatal disease with progressive intra- and extra-hepatic bile ducts inflammation and hepatic fibrosis....
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Therapeutic intervention based on gene sequencing analysis of microbial 16S ribosomal RNA of the intrauterine microbiome improves pregnancy outcomes in IVF patients: a prospective cohort study
PurposeA Lactobacillus -dominated microbiota in the endometrium was reported to be associated with favorable reproductive outcomes. We investigated...
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16S rRNA sequencing analysis of the oral and fecal microbiota in colorectal cancer positives versus colorectal cancer negatives in Iranian population
BackgroundColorectal cancer (CRC) poses a significant healthcare challenge, accounting for nearly 6.1% of global cancer cases. Early detection,...
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Analysis of 16s rRNA Gene Sequencing in Feces: The Impact of Bariatric Surgery on the Gut Microbiota in Patients with Obesity
PurposeObesity is a risk factor for many chronic diseases. This study aimed to investigate the effect of bariatric surgery on the gut microbiota from...
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Comparison of 16S rRNA gene sequencing microbiota among children with serological IgE-mediated food hypersensitivity
BackgroundWe hypothesized that specific food hypersensitivity (FH) in children is linked to specific gut microbiota. The aim of our study was to...
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Routine use of 16S rRNA PCR and subsequent sequencing from blood samples in septic shock: about two case reports of Capnocytophaga canimorsus infection in immunocompetent patients
BackgroundCapnocytophaga canimorsus infection happens frequently in immunosuppressed patients with reported domestic animal bites. Clinical...
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The gut microbiota composition in patients with right- and left-sided colorectal cancer and after curative colectomy, as analyzed by 16S rRNA gene amplicon sequencing
BackgroundGut pathological microbial imbalance or dysbiosis is closely associated with colorectal cancer. Although there are observable differences...
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16S rRNA seq-identified Corynebacterium promotes pyroptosis to aggravate diabetic foot ulcer
BackgroundDiabetic foot ulcer (DFU) is one of the main chronic complications caused by diabetes, leading to amputation in severe cases. Bacterial...
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16S rRNA sequencing-based evaluation of the protective effects of Hua-Zhuo-Jie-Du on rats with chronic atrophic gastritis
BackgroundDisturbance of the intestinal flora is a pathogenic factor for chronic atrophic gastritis (CAG). Hua-Zhuo-Jie-Du (HZJD) has been shown to...
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Impact of 16S rDNA sequencing on clinical treatment decisions: a single center retrospective study
BackgroundPCRs targeting 16S ribosomal DNA (16S PCR) followed by Sanger’s sequencing can identify bacteria from normally sterile sites and complement...
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Coinfection of Clostridium perfringens and Escherichia coli in gas-producing perianal abscess diagnosed by 16S rDNA sequencing: a case report
BackgroundGas-producing perianal abscess raises the possibility of clostridial infection, with Clostridium perfringens being the most common...
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Role of 16S Ribosomal RNA Analysis in Identification of Microorganisms in Febrile Urinary Tract Infection of Infants
ObjectivesTo assess the role of 16S ribosomal RNA analysis in microbial identification in febrile infants under six months of age diagnosed with UTI,...
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Improved Diagnostics in Bacterial Neonatal Meningitis Using a Next-Generation Sequencing Platform
IntroductionBacterial meningitis in infants is an infrequent but life-threatening condition. Empiric therapy should begin as soon as meningitis is...
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16S rRNA gene amplicon sequencing of gut microbiota in gestational diabetes mellitus and their correlation with disease risk factors
PurposeAlthough the gut microbiota (GM) are associated with various diseases, their role in gestational diabetes mellitus (GDM) remains...
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Next-generation sequencing of 16S rRNA for identification of invasive bacterial pathogens in a formalin-fixed paraffin-embedded placental specimen: a case report of perinatal fulminant Streptococcus pyogenes infection
Intrauterine infection is one of the most important causes of maternal death. In perinatal emergency, we often miss an opportunity to obtain culture...