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SfuPKS2, a novel type III polyketide synthase from Sargassum fusiforme, shows high substrate specificity
A type III polyketide synthase (SfuPKS2) from the edible seaweed Sargassum fusiforme was cloned and biochemically characterized. SfuPKS2 can only...
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Rational engineering of an elevator-type metal transporter ZIP8 reveals a conditional selectivity filter critically involved in determining substrate specificity
Engineering of transporters to alter substrate specificity as desired holds great potential for applications, including metabolic engineering....
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Flexible active-site loops fine-tune substrate specificity of hyperthermophilic metallo-oxidases
Hyperthermophilic (‘superheat-loving’) archaea found in high-temperature environments such as Pyrobaculum aerophilum contain multicopper oxidases...
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Analysis of substrate specificity of cytochrome P450 monooxygenases involved in trichothecene toxin biosynthesis
Trichothecenes are a structurally diverse family of toxic secondary metabolites produced by certain species of multiple fungal genera. All...
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Determinants of substrate specificity in a catalytically diverse family of acyl-ACP thioesterases from plants
BackgroundACYL-LIPID THIOESTERASES (ALTs) are a subclass of plastid-localized, fatty acyl-acyl carrier protein (ACP) thioesterase enzymes from...
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CAND1 inhibits Cullin-2-RING ubiquitin ligases for enhanced substrate specificity
Through targeting essential cellular regulators for ubiquitination and serving as a major platform for discovering proteolysis-targeting chimera...
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Characterization of Prolyl-4-Hydroxylase Substrate Specificity Using Pichia pastoris as an Efficient Eukaryotic Expression System
The use of eukaryotic expression systems facilitates the heterologous expression of complex eukaryotic proteins in their post-translationally...
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Modification of substrate specificity of l-arginine oxidase for detection of l-citrulline
Enzymatic detection of citrulline, a potential biomarker for various diseases, is beneficial. However, determining citrulline levels requires...
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Map** the determinants of catalysis and substrate specificity of the antibiotic resistance enzyme CTX-M β-lactamase
CTX-M β-lactamases are prevalent antibiotic resistance enzymes and are notable for their ability to rapidly hydrolyze the extended-spectrum...
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Technical pipeline for screening microbial communities as a function of substrate specificity through fluorescent labelling
The study of specific glycan uptake and metabolism is an effective tool in aiding with the continued unravelling of the complexities in the human gut...
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Glycogen debranching pathway deduced from substrate specificity of glycogen debranching enzyme
Glycogen debranching enzyme (GDE) is bifunctional in that it exhibits both 4-α-glucanotransferase and amylo-α-1,6-glucosidase activity at two...
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Oligomeric Structure of Yeast and Other Invertases Governs Specificity
Invertases, or β-fructofuranosidases, are metabolic enzymes widely distributed among plants and microorganisms that hydrolyze sucrose and release...
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Modification of the substrate specificity of leucine dehydrogenase by site-directed mutagenesis based on biocomputing strategies
Synthesis of chiral amines by amine dehydrogenase has the advantages of environmental friendliness, high stereoselectivity, and mild reaction...
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Discovery of NSD2 non-histone substrates and design of a super-substrate
The human protein lysine methyltransferase NSD2 catalyzes dimethylation at H3K36. It has very important roles in development and disease but many...
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Identification and recombinant production of a flavonoid glucosyltransferase with broad substrate specificity from Vaccinium corymbosum
Glucosyltransferases (GTs) are enzymes that use UDP-glucose to glucosylate wide variety of substrates, including the aglycones of anthocyanins....
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A Novel Thermo-Alkaline Stable GDSL/SGNH Esterase with Broad Substrate Specificity from a Deep-Sea Pseudomonas sp.
Marine environments harbor a plethora of microorganisms that represent a valuable source of new biomolecules of biotechnological interest. In...
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A survey of substrate specificity among Auxiliary Activity Family 5 copper radical oxidases
There is significant contemporary interest in the application of enzymes to replace or augment chemical reagents toward the development of more...
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Effect of Gln469 on the Activity and Substrate Specificity of the N-glycosyltransferase from Actinobacillus pleuropneumoniae
AbstractN-Glycosyltransferase from Actinobacillus pleuropneumoniae (ApNGT) belongs to a novel class of N-glycosylation-catalyzing enzymes and has...
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Enhanced catalytic efficiency and substrate specificity of Streptomyces griseus trypsin by evolution-guided mutagenesis
Streptomyces griseus trypsin (SGT) is a bacteria-sourced trypsin that could be potentially applied to industrial insulin productions. However, SGT...
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Structural mechanisms of autoinhibition and substrate recognition by the ubiquitin ligase HACE1
Ubiquitin ligases (E3s) are pivotal specificity determinants in the ubiquitin system by selecting substrates and decorating them with distinct...
