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Full-Length Single-Cell RNA-Sequencing with FLASH-seq
The single-cell RNA-sequencing (scRNA-seq) field has evolved tremendously since the first paper was published back in 2009 (Tang et al. Nat Methods...
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Protoplast Isolation for Plant Single-Cell RNA-seq
The growth and development of plants depends on diversified gene expression in different cell types. Compared to traditional bulk RNA sequencing,...
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Single-Cell Sequencing of 3′ RNA Transcripts
Single-cell RNA sequencing (scRNA-seq) enables the measurement of RNA expressed from individual cells within a tissue or population. RNA expression...
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LAST-seq: single-cell RNA sequencing by direct amplification of single-stranded RNA without prior reverse transcription and second-strand synthesis
Existing single-cell RNA sequencing (scRNA-seq) methods rely on reverse transcription (RT) and second-strand synthesis (SSS) to convert...
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Plant Single-Cell/Nucleus RNA-seq Workflow
Single-cell transcriptomics technologies allow researchers to investigate how individual cells, in complex multicellular organisms, differentially...
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Computational Analysis of Single-Cell RNA-Seq Data
Single-cell RNA sequencing (scRNA-seq) is gaining popularity as this allows you to profile a large number of individual cells. However, as the volume...
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Analysis of Single-Cell RNA-seq Data
As single-cell RNA sequencing experiments continue to advance scientific discoveries across biological disciplines, an increasing number of analysis...
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Single Cell RNA-Sequencing in Arabidopsis Root Tissues
Droplet-based single-cell RNA-sequencing (scRNA-seq)Single-cell RNA-sequencing (scRNA-seq) empowers transcriptomicTranscriptomics profiling with an...
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Single-cell RNA-seq of maize meiocytes and pollen grains
RNA-sequencing (RNA-seq) provides invaluable knowledge on developmental pathways and the effects of mutant phenotypes. Plant reproductive cells have...
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A MATQ-seq-Based Protocol for Single-Cell RNA-seq in Bacteria
Microbes exhibit an extraordinary capacity to adapt their physiology to different environments using phenotypic heterogeneity. However, the majority...
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ProBac-seq, a bacterial single-cell RNA sequencing methodology using droplet microfluidics and large oligonucleotide probe sets
Methods that measure the transcriptomic state of thousands of individual cells have transformed our understanding of cellular heterogeneity in...
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Bulk and Single-Cell RNA-Seq Analyses for Studies of Spermatogonia
Robust methods have been developed that leverage next-generation sequencing (NGS) to measure abundance of all mRNAs (RNA-seq) in samples as small as...
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Single cell RNA-seq: a novel tool to unravel virus-host interplay
Over the last decade, single cell RNA sequencing (scRNA-seq) technology has caught the momentum of being a vital revolutionary tool to unfold...
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Identification of cell barcodes from long-read single-cell RNA-seq with BLAZE
Long-read single-cell RNA sequencing (scRNA-seq) enables the quantification of RNA isoforms in individual cells. However, long-read scRNA-seq using...
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NRTPredictor: identifying rice root cell state in single-cell RNA-seq via ensemble learning
BackgroundSingle-cell RNA sequencing (scRNA-seq) measurements of gene expression show great promise for studying the cellular heterogeneity of rice...
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Benchmarking algorithms for joint integration of unpaired and paired single-cell RNA-seq and ATAC-seq data
BackgroundSingle-cell RNA-sequencing (scRNA-seq) measures gene expression in single cells, while single-nucleus ATAC-sequencing (snATAC-seq)...
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RNA Sequencing (RNA-seq)
RNA sequencing (or popularly known as RNA-seq) or whole transcriptome shortgun sequencing (WTSS) is a useful next-generation sequencing (NGS)...
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Single Cell Micro RNA Sequencing Library Preparation
Micro RNAs represent important post-transcriptional regulators in health and are involved in the onset of many diseases. Therefore, the further...
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Single-Cell RNA-seq Analysis of a Human Embryonic Stem Cell to Endothelial Cell System Based on Transcription Factor Overexpression
BackgroundHuman embryonic stem cell (hESC)-derived endothelial cells (ECs) possess therapeutic potential in many diseases. Cytokine supplementation...
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Preparation of Drosophila Ovarioles for Single-Cell RNA Sequencing
The production of eggs in the Drosophila ovary requires complex interactions between multiple cell types that coexist within the same solid tissue....