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Compact CRISPR genetic screens enabled by improved guide RNA library cloning
CRISPR genome editing approaches theoretically enable researchers to define the function of each human gene in specific cell types, but challenges...
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Optimized RNA-targeting CRISPR/Cas13d technology outperforms shRNA in identifying functional circRNAs
Short hairpin RNAs (shRNAs) are used to deplete circRNAs by targeting back-splicing junction (BSJ) sites. However, frequent discrepancies exist...
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Directed mutagenesis in plants through genome editing using guide RNA library
Plants with genetic diversity have a higher rate of resilience and adaptation to environmental change. Crop species need to diversify genetically if...
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A novel vector-based RNAi method using mouse U6 promoter-driven shRNA expression in the filamentous fungus Blakeslea trispora
PurposeThere are several studies on the use of RNA interference (RNAi) for gene function analysis in fungi. However, most studies on filamentous...
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Enzymatic construction of shRNA library from oligonucleotide library
BackgroundShort hairpin RNAs (shRNAs) expressed from vectors have been used as an effective means of exploiting the RNA interference (RNAi) pathway...
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Kinase shRNA screening reveals that TAOK3 enhances microtubule-targeted drug resistance of breast cancer cells via the NF-κB signaling pathway
BackgroundChemotherapy is currently one of the most effective treatments for advanced breast cancer. Anti-microtubule agents, including taxanes,...
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A knock down strategy for rapid, generic, and versatile modelling of muscular dystrophies in 3D-tissue-engineered-skeletal muscle
BackgroundHuman iPSC-derived 3D-tissue-engineered-skeletal muscles (3D-TESMs) offer advanced technology for disease modelling. However, due to the...
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FOXP3 promote the progression of glioblastoma via inhibiting ferroptosis mediated by linc00857/miR-1290/GPX4 axis
The oncogenic properties of members belonging to the forkhead box (FOX) family have been extensively documented in different types of cancers. In...
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RNA-binding protein RBM5 plays an essential role in acute myeloid leukemia by activating the oncogenic protein HOXA9
BackgroundThe oncogenic protein HOXA9 plays a critical role in leukemia transformation and maintenance, and its aberrant expression is a hallmark of...
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CREB5 promotes the proliferation and self-renewal ability of glioma stem cells
Glioblastoma multiforme (GBM) is the most fatal form of brain cancer in humans, with a dismal prognosis and a median overall survival rate of less...
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Effect of downregulated citrate synthase on oxidative phosphorylation signaling pathway in HEI-OC1 cells
BackgroundCitrate Synthase ( Cs ) gene mutation (locus ahL4 ) has been found to play an important role in progressive hearing loss of A/J mice. HEI-OC1...
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The glutathione S-transferase Gstt1 drives survival and dissemination in metastases
Identifying the adaptive mechanisms of metastatic cancer cells remains an elusive question in the treatment of metastatic disease, particularly in...
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SMOC2 promotes aggressive behavior of fibroblast-like synoviocytes in rheumatoid arthritis through transcriptional and post-transcriptional regulating MYO1C
Fibroblast-like synoviocytes (FLSs), play a key role in perpetuating synovial inflammation and bone erosion in rheumatoid arthritis (RA), however,...
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Plasma non-transferrin-bound iron uptake by the small intestine leads to intestinal injury and intestinal flora dysbiosis in an iron overload mouse model and Caco-2 cells
Iron overload often occurs during blood transfusion and iron supplementation, resulting in the presence of non-transferrin-bound iron (NTBI) in host...
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CRISPR screen identifies the role of RBBP8 in mediating unfolded protein response induced liver damage through regulating protein synthesis
Unfolded protein response (UPR) maintains the endoplasmic reticulum (ER) homeostasis, survival, and physiological function of mammalian cells....
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Enhanced RNA knockdown efficiency with engineered fusion guide RNAs that function with both CRISPR-CasRx and hammerhead ribozyme
BackgroundCRISPR-Cas13 is a newly emerging RNA knockdown technology that is comparable to RNAi. Among all members of Cas13, CasRx degrades RNA in...
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Inhibition of CARM1 suppresses proliferation of multiple myeloma cells through activation of p53 signaling pathway
BackgroundMultiple myeloma (MM) is a malignant proliferative disease of plasma cells, the incidence of which is increasing every year and remains...
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Analysis of asymptomatic Drosophila models for ALS and SMA reveals convergent impact on functional protein complexes linked to neuro-muscular degeneration
BackgroundSpinal Muscular Atrophy (SMA) and Amyotrophic Lateral Sclerosis (ALS) share phenotypic and molecular commonalities, including the fact that...
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Ionizing irradiation-induced Fgr in senescent cells mediates fibrosis
The role of cellular senescence in radiation-induced pulmonary fibrosis (RIPF) and the underlying mechanisms are unknown. We isolated...
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Screening and Characterization of Functional circRNAs in Neuronal Cultures
This chapter describes a methodology for the screening and characterization of functional circRNAs, particularly in the context of neural circuit...