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Neutralizing anti-diphtheria toxin scFv produced by phage display
BackgroundDiphtheria can be prevented by vaccination, but some epidemics occur in several places, and diphtheria’s threat is considerable....
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Therapeutic potential of a novel IP-10-(anti-HER2 scFv) fusion protein for the treatment of HER2-positive breast cancer
ObjectivesInterferon-γ-inducible protein 10 (IP-10) is a potent antitumor agent and acts by its angiostatic and immunomodulatory properties. IP-10...
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Bacterial production and biophysical characterization of a hard-to-fold scFv against myeloid leukemia cell surface marker, IL-1RAP
BackgroundInterleukin-1 receptor accessory protein (IL-1RAP) is one of the most promising therapeutic targets proposed for myeloid leukemia....
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Construction of Human Immune and Naive scFv Phage Display Libraries
Antibody phage display is a widely used in vitro selection technology for the generation of human recombinant antibodies and has yielded thousands of...
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Combining of synthetic VHH and immune scFv libraries for pregnancy-associated glycoproteins ELISA development
AbstractNanobodies (VHH) from camelid antibody libraries hold great promise as therapeutic agents and components of immunoassay systems. Synthetic...
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Design and construction of scFv-PE35KDEL as a novel immunotoxin against human epidermal growth factor receptor 2 for cancer therapy
ProposeHuman epidermal growth factor receptor 2 (HER2) is overexpressed on the surface of some kinds of cancer cells including breast cancer. In this...
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Designing a Humanized Immunotoxin Based on HER2 Specific scFv and DFF40 Toxin Against Breast Cancer: An In-Silico Study
Breast cancer is the most frequent cancer in women worldwide. Human epidermal growth factor receptor 2 (HER2) is a receptor which is overexpressed in...
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Tuning the ignition of CAR: optimizing the affinity of scFv to improve CAR-T therapy
How single-chain variable fragments (scFvs) affect the functions of chimeric antigen receptors (CARs) has not been well studied. Here, the components...
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Specific anti-SARS-CoV-2 S1 IgY-scFv is a promising tool for recognition of the virus
As severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) continues to spread globally, a series of vaccines, antibodies and drugs have been...
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Facile One-Step Generation of Camelid VHH and Avian scFv Libraries for Phage Display by Golden Gate Cloning
Since its development in the 1980s, the Nobel Prize-awarded phage display technology has been one of the most commonly used in vitro selection...
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Optimization of Tris/EDTA/Sucrose (TES) periplasmic extraction for the recovery of functional scFv antibodies
Single-chain variable fragments (scFvs) have gained increased attention among researchers in both academic and industrial fields owing to simple...
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Single-chain variable fragment-based immunoassay for bioactive natural products
Bioactive natural products (BNPs) from medicinal plants are one of the most successful sources for new drugs. As BNPs contain the beneficial and...
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Production and applications of fluorobody from redox-engineered Escherichia coli
AbstractEfficient selection and production of antibody fragments in microbial systems remain to be a challenging process. To optimize microbial...
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Cloning, Expression and One-Step Purification of a Novel IP-10-(anti-HER2 scFv) Fusion Protein in Escherichia coli
Breast cancer is the most common cancer in women, worldwide. The correlation between breast cancer malignancy and human epidermal growth factor 2...
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Inhibition of beet necrotic yellow vein virus by the expression of a single-chain variable fragment antibody in sugar beet plants
Rhizomania caused by the Beet necrotic yellow vain virus (BNYVV) is the most damaging disease of sugar beet worldwide. Antibody-based resistance was...
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Antibody and antibody fragments site-specific conjugation using new Q-tag substrate of bacterial transglutaminase
During the last few years Antibody-Drug Conjugates (ADCs) have become one of the most active and very promising therapeutic weapons. Lessons learned...
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Antibody Batch Cloning
The antigen-binding ability of each antibody clone selected by phage display is usually initially ranked by a screening ELISA using monovalent scFv...
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Extracellular domains of CARs reprogramme T cell metabolism without antigen stimulation
Metabolism is an indispensable part of T cell proliferation, activation and exhaustion, yet the metabolism of chimeric antigen receptor (CAR)-T cells...
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Genome-scale metabolic model-based engineering of Escherichia coli enhances recombinant single-chain antibody fragment production
PurposeEscherichia coli is an attractive and cost-effective cell factory for producing recombinant proteins such as single-chain variable fragments...
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A Novel Specific Single-Chain Variable Fragment Diagnostic System for Viral Hemorrhagic Septicemia Virus
Viral hemorrhagic septicemia virus (VHSV), one of the most important viral marine pathogens worldwide, has a broad range of hosts, such as members of...
