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sRNA Structural Modeling Based on NMR Data
Small non-coding RNAs (sRNAs) play vital roles in gene expression regulation and RNA interference. To comprehend their molecular mechanisms and...
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Investigation of sRNA-mRNA Interactions in Bacillus subtilis In Vivo
In this chapter, we describe in vivo methods for the analysis of interactions between an sRNA and its target mRNA in B. subtilis. All these methods...
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Directed Screening for sRNA Targets in E. coli Using a Plasmid Library
A large number of bacterial small regulatory RNAs (sRNAs) modulate gene expression by base pairing to a target mRNA, affecting its translation or...
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Oral administration of the herbal oligonucleotide XKC-sRNA-h3 prevents angiotensin II-induced hypertension in mice
Hypertension has become a growing public health concern worldwide. In fact, hypertension is commonly associated with increased morbidity and...
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In Vitro Methods for the Investigation of sRNA-mRNA Interactions in Bacillus subtilis
So far, in Bacillus subtilis, only four trans-encoded and 11 cis-encoded sRNAs and their targets have been investigated in detail, the majority of...
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Orally administered BZL-sRNA-20 oligonucleotide targeting TLR4 effectively ameliorates acute lung injury in mice
The global COVID-19 pandemic emerged at the end of December 2019. Acute respiratory distress syndrome (ARDS) and acute lung injury (ALI) are common...
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A Regulatory sRNA rli41 is Implicated in Cell Adhesion, Invasion and Pathogenicity in Listeria monocytogenes
AbstractSmall regulatory RNAs (sRNAs) play important roles as post-transcriptional regulators throughout the life activities of bacteria. To explore...
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An Integrated Affinity Chromatography-Based Approach to Unravel the sRNA Interactome in Nitrogen-Fixing Rhizobia
The activity mechanism and function of bacterial base-pairing small non-coding RNA regulators (sRNAs) are largely shaped by their main interacting...
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Synthetic fused sRNA for the simultaneous repression of multiple genes
Efficient control over multiple gene expression still presents a major challenge. Synthetic sRNA enables targeted gene expression control in trans...
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Improving Bacterial sRNA Identification By Combining Genomic Context and Sequence-Derived Features
Bacterial small non-coding RNAs (sRNAs) are ubiquitous regulatory RNAs involved in controlling several cellular processes by targeting multiple...
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Silencing of Curlin Protein via M13 Phagemid-Mediated Synthetic sRNA Expression Reduces Virulence in the Avian Pathogenic E. coli (APEC)
Curli fimbriae, a virulent factor of the Avian Pathogenic Escherichia coli (APEC), is responsible for adhesion, biofilm formation, and colonization...
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srnaMapper: an optimal map** tool for sRNA-Seq reads
BackgroundSequencing is the key method to study the impact of short RNAs, which include micro RNAs, tRNA-derived RNAs, and piwi-interacting RNA,...
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Fluorescent In Situ Detection of Small RNAs in Plants Using sRNA-FISH
Plant small RNAs are 21–24 nucleotide, noncoding RNAs that function as regulators in plant growth and development. Colorimetric detection of plant...
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Modulatory Impact of the sRNA Mcr11 in Two Clinical Isolates of Mycobacterium tuberculosis
Mycobacterium tuberculosis (Mtb) is a successful pathogen causing tuberculosis (TB) disease in humans. It has been shown, that some circulating...
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Dynamics and Function of sRNA/mRNAs Under the Scrutiny of Computational Simulation Methods
Molecular dynamics simulations have proved extremely useful in investigating the functioning of proteins with atomic-scale resolution. Many...
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Discovering Novel Bacterial Small RNA by RNA-seq Analysis Toolkit ANNOgesic
ANNOgesic is an RNA-seq analysis pipeline that can detect sRNAs and many other genomic features in bacteria and archaea. In addition to listing sRNA...
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CRISPR Interference-Based Functional Small RNA Genomics
Small RNAs (sRNAs) are versatile regulators universally present in species across the prokaryotic kingdom, yet their functional characterization...
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Evaluation of 5′-End Phosphorylation for Small RNA Stability and Target Regulation In Vivo
Bacterial small RNAs (sRNAs) can be equipped at the 5′ end with triphosphate (5′PPP) or monophosphate (5′P) groups, depending on whether they are...