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Selection and evaluation of reference genes for qRT-PCR of Scutellaria baicalensis Georgi under different experimental conditions
Scutellaria baicalensis Georgi is a famous medicinal plant with its dried roots having been used as a traditional Chinese medicinal for more than...
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Selection and verification of reliable internal reference genes in stem development of herbaceous peony (Paeonia lactiflora Pall.)
Herbaceous peony ( Paeonia lactiflora Pall.) has emerged in the cut flower market due to its beautiful appearance. The bending flower stems caused by...
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Genome-wide analysis of the NAC gene family and functional verification of the DcNAC043s in Dendrobium catenatum
The NAC (NAM, ATAF1/2 and CUC2) domain protein plays an important role in plant growth and development. The function of members of NAC gene family...
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Public-transcriptome-database-assisted selection and validation of reliable reference genes for qRT-PCR in rice
Accurate quantitative reverse transcription PCR (qRT-PCR) requires reliable reference genes whose expression does not vary in different tissues and...
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Molecular Detection and Identification of Pathogens, Pathotypes, and Genes
The accurate, rapid detection and identification of the pathogens is essential for effective management of crucifers’ diseases. The traditional...
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Discovery and verification of mmu_Circ_26986/hsa_Circ_0072463 as a potential biomarker and intervention target for sepsis-associated acute kidney injury
Approximately 60% of septic patients developed acute kidney injury (AKI). The mortality rate of septic AKI (SA-AKI) is two to three times higher than...
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The non-target site resistance mechanism to Penoxsulam in Echinochloa crus-galli var. zelayensis
BackgroudEchinochloa curs-galli (barnyardgrass) is the most prominent weed in rice fields, and its herbicide resistance seriously threatens rice...
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Validation of suitable reference genes for qRT-PCR in cabbage (Brassica oleracea L.) under different abiotic stress experimental conditions
Cabbage ( Brassica oleracea ) is one of the most important vegetable crops worldwide. qRT-PCR is a sensitive technique for gene expression studies and...
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Transcriptome analysis of drought tolerance and utility assessment of DnaJ gene expression as a potential index for drought resistance evaluation in maize
Drought tolerance is a very complex trait, controlled by multiple genes, difficult to identify and evaluate in maize. Here, we employed RNA-seq...
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Molecular identification and functional verification of SPL9 and SPL15 of Lilium
The transformation of plants from juveniles to adults is a key process in plant growth and development, and the main regulatory factors are miR156...
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Comparative transcriptome analysis to reveal key ethylene genes involved in a Lonicera macranthoides mutant
BackgroundLonicera macranthoides Hand.-Mazz. is an important medicinal plant. **anglei-type (XL) L. macranthoides was formed after many years of...
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Anti-Inflammatory Mechanism of Walnut Meal Peptide/ Ellagic Acid Nanoparticles on LPS-Stimulated RAW264.7 Cells by Inhibition of NF-κB and MAPK Activation
The combined use of walnut meal peptides and ellagic acid demonstrated synergistic anti-inflammatory properties, which could be enhanced through...
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Systematic identification and expression analysis of bHLH gene family reveal their relevance to abiotic stress response and anthocyanin biosynthesis in sweetpotato
BackgroundbHLH transcription factors play significant roles in regulating plant growth and development, stress response, and anthocyanin...
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Transcriptome analysis and identification of the low potassium stress-responsive gene SiSnRK2.6 in foxtail millet (Setaria italica L.)
Key messageThe transcriptome is beneficial for dissecting the mechanism of millet in response to low potassium stress and SiSnRK2.6 was identified...
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Rapidly mining candidate cotton drought resistance genes based on key indicators of drought resistance
BackgroundFocusing on key indicators of drought resistance is highly important for quickly mining candidate genes related to drought resistance in...
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Protocols to Study Host-Pathosystems
Crucifers’ host-pathosystem has become a favorable model system of biologists to get deeper insight into various biological functions operating by...
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The SWEET gene family in watermelon: genome-wide identification, phylogeny, duplication and expression analyses in male sterile buds and under sucrose, fructose and glucose treatments
Sugars will eventually be exported transporters (SWEETs) have been confirmed to be involved in the regulation of plant growth, development and stress...
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Insights into heat response mechanisms in Clematis species: physiological analysis, expression profiles and function verification
Key messageOur results provide insights into heat response mechanisms among Clematis species. Overexpressing CvHSFA2 enhanced the heat resistance of...
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CircRNA/lncRNA–miRNA–mRNA network and gene landscape in calcific aortic valve disease
BackgroundCalcific aortic valve disease (CAVD) is a common valve disease with an increasing incidence, but no effective drugs as of yet. With the...
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Genome-wide identification of the basic leucine zipper transcription factor genes related to starch synthesis in wheat (Triticum aestivum L.)
In plants, the basic leucine zipper (bZIP) family of transcription factors is known for its large size and diversity. Many studies have shown that...
