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Identification of a novel thermostable transaminase and its application in L-phosphinothricin biosynthesis
AbstractTransaminase (TA) is a crucial biocatalyst for enantioselective production of the herbicide L-phosphinothricin (L-PPT). The use of enzymatic...
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Sulfadiazine and phosphinothricin selection systems optimised for the transformation of tobacco BY-2 cells
Key messageWe extended the applicability of the BY-2 cell line as a model by introducing two new selection systems. Our protocol provides guidelines...
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Expression of l-phosphinothricin synthesis enzymes in Pichia pastoris for synthesis of l-phosphinothricin
With the ban of highly toxic herbicides, such as paraquat and glyphosate, phosphinothricin (PPT) is becoming the most popular broad-spectrum and...
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Aptamer-linked immobilized sorbent assay for detecting GMO marker, phosphinothricin acetyltransferase (PAT)
BackgroundDevelopment of genetically modified crops has rapidly increased in last few years. The most widely grown GM crops express genes that confer...
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Development and validation of a multiplex real-time PCR method for screening genetically modified plants
The diversity of genetically modified plants used for food and feed is increasing worldwide. For the detection and control of these products,...
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A bifunctional selectable marker for wheat transformation contributes to the characterization of male-sterile phenotype induced by a synthetic Ms2 gene
Key messageAn engineered selectable marker combining herbicide resistance and yellow fluorescence contributes to the characterization of male-sterile...
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Overexpression of Genes to Enhance Abiotic Stress Tolerance in Arabidopsis
Abiotic environmental stressors cause various types of damage to plants and cause significant loss in yield. Abiotic stress tolerance in plants...
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Establishment of a chloroplast transformation system in Tisochrysis lutea
Tisochrysis lutea is a haptophyte microalga commonly used as a commercial feed for juvenile fish and shellfish larvae. Genetic modification is of...
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Genetic Engineering: A Powerful Tool for Crop Improvement
Rising population, changing climatic conditions, and various biotic and abiotic stresses are contributors to lowering crop yields. This, in turn, has...
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Genome Editing in Species of the Tribe Triticeae with the CRISPR/Cas System
AbstractThe tribe Triticeae includes important agricultural crops, such as bread wheat, durum wheat, barley, rye, and triticale. Research in the...
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Introduction of Multiple Genes via Agrobacterium-Mediated Co-Transformation into Soybean and Confirmation of Insertion Using PCR
Agrobacterium -mediated transformation is used to introduce foreign genes into soybeans. However, improved transformation processes are needed to...
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Protoplast Isolation, Transformation, and Regeneration for Forage and Turf Grasses
Forage and turf grasses are widely grown and contribute significantly to sustainable agriculture. This chapter describes a protocol for protoplast...
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Efficient reductive amination process for enantioselective synthesis of L-phosphinothricin applying engineered glutamate dehydrogenase
The objective of this study was to identify and exploit a robust biocatalyst that can be applied in reductive amination for enantioselective...
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Chloroplast Genetic Engineering of a Unicellular Green Alga Haematococcus pluvialis with Expression of an Antimicrobial Peptide
The purpose of this study was to express an antimicrobial peptide in the chloroplast to further develop the plastid engineering of H. pluvialis ....
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Introduction of the Nicotiana protein kinase (NPK1) gene by combining Agrobacterium-mediated transformation and recurrent somatic embryogenesis to enhance salt tolerance in cauliflower
Cauliflower is exposed to various biotic and abiotic stresses, including increased salinity due to the intensive irrigation of crops....
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Two insecticidal neurotoxins from parasitoid wasp Habrobracon hebetor venom and their potential use in biocontrol
The utilization of diverse entomopathogens like bacteria, viruses, or fungi holds promise in the biological control of insect pests. One approach to...
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High efficiency transformation and mutant screening of the laurel wilt pathogen, Raffaelea lauricola
AbstractThe fungal pathogen, Raffaelea lauricola , is the causative agent of laurel wilt, a devastating disease affecting the Lauraceae family. The...
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Genetic Improvement of Poplar
Poplars are crucial to world forestry. In addition to being grown as an agronomy crop, they offer plywood materials, sports stuffs, raw materials for...
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Mutation of storage protein gene using CRISPR/Cas9 removed α′-subunit of β-conglycinin in soybean seeds
β-Conglycinin (7S) and glycinin (11S) are the two major storage proteins in soybean seeds. Storage proteins occupy a large portion of soybean seeds,...
