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Tissue Culture Techniques for Saffron Improvement
Several clonal propagation methods are able to produce true-to-type clones of saffron plants under aseptic controlled conditions. Saffron in vitro...
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3D Cell Culture Techniques
To better understand human physiology and its development and pathological conditions, in vitro cell culture models are recognised as effective...
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Spatial analysis of tissue immunity and vascularity by light sheet fluorescence microscopy
The pathogenesis of cancer and cardiovascular diseases is subjected to spatiotemporal regulation by the tissue microenvironment. Multiplex...
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Dense 4D nanoscale reconstruction of living brain tissue
Three-dimensional (3D) reconstruction of living brain tissue down to an individual synapse level would create opportunities for decoding the dynamics...
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Defined Human Leukemic CD34+ Liquid Cultures to Study HDAC/Transcriptional Repressor Complexes
Defined human primary cell model systems with growth dependence on oncogenes are highly requested to investigate tumor pathogenesis and to validate...
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Tissue culture-based genetic improvement of fava bean (Vicia faba L.): analysis on previous achievements and future perspectives
Fava bean is an extremely important legume and serves immense potential to function as an ingredient as pulse proteins in human diet. Bearing the...
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CryoGrid-PIXUL-RNA: high throughput RNA isolation platform for tissue transcript analysis
BackgroundDisease molecular complexity requires high throughput workflows to map disease pathways through analysis of vast tissue repositories. Great...
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Three Dimensional Bioprinting for Hepatic Tissue Engineering: From In Vitro Models to Clinical Applications
Fabrication of functional organs is the holy grail of tissue engineering and the possibilities of repairing a partial or complete liver to treat...
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Sample Preparation from Plant Tissue for Gas Chromatography–Mass Spectrometry (GC-MS)we
Metabolites are intermediate products formed during metabolism. Metabolites play different roles, including providing energy, supporting structure,...
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Continuous human iPSC-macrophage mass production by suspension culture in stirred tank bioreactors
Macrophages derived from human induced pluripotent stem cells (iPSCs) have the potential to enable the development of cell-based therapies for...
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Real-Time Cell Cycle Imaging in a 3D Cell Culture Model of Melanoma, Quantitative Analysis, Optical Clearing, and Mathematical Modeling
Aberrant cell cycle progression is a hallmark of solid tumors. Therefore, cell cycle analysis is an invaluable technique to study cancer cell...
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Canine Detection of Cancer in Humans: Expectations Versus Reality
Easily accessible screening methods play a crucial role in the early detection of cancer. Over the last few decades, alternative cancer screening...
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Development of ionic liquid-coated PLGA nanoparticles for applications in intravenous drug delivery
Polymeric nanoparticles (NPs) are a promising platform for medical applications in drug delivery. However, their use as drug carriers is limited by...
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Biotechnological approaches for the production of hypericin and other important metabolites from the genus Hypericum
Hypericin, a polycyclic naphthodianthrone and active plant pigment with the molecular formula C 30 H 16 O 8 , is a crucial phytochemical extracted from the...
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Chemical Mutagenesis of Embryogenic Cell Suspensions of Coffea arabica L. var. Catuaí Using EMS and NaN3
Chemical mutagens, such as ethyl methanesulfonate (EMS) and sodium azide (NaN3), interact with DNA and can primarily induce single base modifications...
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Genetic heterogeneity in p53-null leukemia increases transiently with spindle assembly checkpoint inhibition and is not rescued by p53
Chromosome gains or losses often lead to copy number variations (CNV) and loss of heterozygosity (LOH). Both quantities are low in hematologic...
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Strategy for the Identification of Host-Defense Peptides in Frog Skin Secretions with Therapeutic Potential as Antidiabetic Agents
Several amphibian peptides that were first identified on the basis of their antimicrobial or cytotoxic properties have subsequently shown potential...
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Assays for Studying Pseudomonas aeruginosa Secreted Proteases
Proteolytic activity plays an essential role in Pseudomonas aeruginosa adaptation and survival in challenging environments, including the infection...
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BIT® bioreactor increases in vitro multiplication of quality shoots in sugarcane (Saccharum spp. variety LAICA 04-809)
The multiplication of sugarcane ( Saccharum spp.) variety LAICA 04-809 by direct organogenesis was evaluated using four cultivation systems: (1) the...
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Generating human bone marrow organoids for disease modeling and drug discovery
The bone marrow supports and regulates hematopoiesis, responding to physiological requirements for blood cell production over ontogeny and during...
