Search
Search Results
-
Latent Macrophage and Immature B Cell Lines Generated with Hygromycin-Resistant Murine Gammaherpesvirus 68 Genome Expresses Modest Levels of Viral miRNAs
AbstractMurine gammaherpesvirus 68 (MHV68) establishes latency mainly in B cells and causes lymphomas reminiscent of human gammaherpesvirus diseases...
-
A benzaldehyde derivative obtained from Hypoxylon truncatum NBRC 32353 treated with hygromycin B
The ribosome-targeted antifungal agent hygromycin B (HygB) alters the secondary metabolite profiles of fungi. Hypoxylon truncatum NBRC 32353...
-
Hygromycin A derivatives isolated from Streptomyces sp. PC-22 in the rhizosphere soil of Pulsatilla chinensis
On the basis of the one strain-many compounds (OSMAC) strategy, two new hygromycin A derivatives ( 3 , 4 ), together with six known compounds were...
-
Agrobacterium tumefaciens-Mediated Transformation of Rice by Hygromycin Phosphotransferase (hptII) Gene Containing CRISPR/Cas9 Vector
The CRISPR/Cas9 technique for rice genome engineering is gaining momentum and requires a precise gene delivery system. For rice and other crop...
-
Enhanced resistance of Trichoderma harzianum LZDX-32-08 to hygromycin B induced by sea salt
ObjectivesTo determine the effect of sea salt on the resistance of Trichoderma harzianum LZDX-32-08 to hygromycin B and speculate the possible...
-
Stable transformation of the unicellular lichenic green alga Coccomyxa solorinae-saccatae via electroporation
In this study, we have demonstrated the applicability of electroporation for the stable nuclear transformation of Coccomyxa solorinae-saccatae . An...
-
Induction of secondary metabolite production by hygromycin B and identification of the 1233A biosynthetic gene cluster with a self-resistance gene
We found that the protein synthesis inhibitor hygromycin B induced the production of secondary metabolites, including lucilactaene, NG-391,...
-
Establishment and validation of a callus tissue transformation system for German chamomile (Matricaria chamomilla L.)
BackgroundGerman chamomile ( Matricaria chamomilla L.) is an important medicinal plant, and the essential oils in the flowers have various biological...
-
Studies on the callus induction and Agrobacterium-mediated transformation of Asia minor bluegrass (Polypogon fugax)
Asia Minor bluegrass ( Polypogon fugax Nees ex Steud.) is one of the major grass weeds in winter crops in China, with populations having developed...
-
Agrobacterium-mediated in planta transformation of cut coleoptile: a new, simplified, and tissue culture-independent method to deliver the CRISPR/Cas9 system in rice
BackgroundAgrobacterium -mediated transformation and particle bombardment are the two common approaches for genome editing in plant species using...
-
Heterologous expression of SbAP37-transcription factor in Sesamum indicum L. alleviates salt stress and improves antioxidant enzyme activities
Deembryonated cotyledons of Sesame ( Sesamum indicum L.), an oil yielding crop plant, were transformed using Agrobacterium tumefaciens strain LBA4404...
-
Measurement of transgene copy number in transgenic tobacco plants producing human interferon-γ (hIFN-γ) using quantitative real-time PCR
In transgenic plants, the transgene copy numbers can highly affect the level of expression and genetic stability of the transgene. Hence, the first...
-
Split selectable marker systems utilizing inteins facilitate gene stacking in plants
The ability to stack multiple genes in plants is of great importance in the development of crops with desirable traits but can be challenging due to...
-
Agrobacterium tumefaciens-Mediated Transformation Method for Fusarium oxysporum
Agrobacterium tumefaciens-mediated transformation (ATMT) is becoming a popular effective system as an insertional mutagenesis tool in filamentous...
-
Potential of Wheat dwarf virus (Geminiviridae: Mastrevirus) Truncated Promoter for Improvement of Transgene Expression in Rice
Wheat dwarf virus (WDV) is a monocotyledonous plant-infecting virus that causes economical loss to cereal cultivations annually. The objective of...
-
Direct regeneration and genetic transformation studies in Hemidesmus indicus (L.) R. Br. (Indian Sarsaparilla)
Hemidesmus indicus L. R. Br. is a rare and endangered plant exhibits potent medicinal properties explored for the presence of flavoring compound in...
-
A stable method for Agrobacterium-mediated transformation of Mentha piperita and Mentha canadensis using internodal explants
Mentha species are planted worldwide for extraction of their metabolites, including essential oils and phenolic acid compounds, which have...
-
An efficient Agrobacterium tumefaciens-mediated transformation system of Taraxacum kok-saghyz Rodin (Russian dandelion)
Taraxacum kok-saghyz Rodin (TK) is an excellent alternative to Hevea brasiliensis because of its high yield of latex, wide growth range, and short...
-
Establishment of protoplast preparation protocol and genetic transformation system for Sclerotiophoma versabilis
Going into deep study about the molecular mechanism of pathogenesis in Sclerotiophoma versabilis required high-efficiency transformation system. S....
-
Biosynthesis of a Novel Ganoderic Acid in Saccharomyces cerevisiae and Research of its Antitumor Activity
AbstractGanoderic acids (GAs), mainly from the traditional Chinese medicinal Ganoderma lucidum , possess antitumor, anti-metastasis and other...
