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Global Fungal Diversity Estimated from High-Throughput Sequencing
Fungi are key players in vital ecosystem services, spanning carbon cycling, decomposition, and varied plant symbioses. Due to their cryptic...
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High-throughput RNA isoform sequencing using programmed cDNA concatenation
Full-length RNA-sequencing methods using long-read technologies can capture complete transcript isoforms, but their throughput is limited. We...
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Advances of high-throughput sequencing for unraveling biotechnological potential of microalgal-bacterial communities
Although established biotechnological applications of microalgae e.g., the production of high-value metabolites is based on axenic cultures,...
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TrieDedup: a fast trie-based deduplication algorithm to handle ambiguous bases in high-throughput sequencing
BackgroundHigh-throughput sequencing is a powerful tool that is extensively applied in biological studies. However, sequencers may produce...
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Construction of relatedness matrices in autopolyploid populations using low-depth high-throughput sequencing data
Key messageAn improved estimator of genomic relatedness using low-depth high-throughput sequencing data for autopolyploids is developed. Its outputs...
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Identification of bacteria associated with canine otitis externa based on 16S rDNA high-throughput sequencing
Bacteria are regarded as predisposing and perpetuating factors causing otitis externa (OE), whereas auricular anatomy is a predisposing factor. This...
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Sample Preparation for Fungal Community Analysis by High-Throughput Sequencing of Barcode Amplicons
Fungal species participate in vast numbers of processes in the landscape around us. However, their cryptic mycelial growth, inside various substrates...
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DNA virome of Brazilian sugarcane germplasm via high-throughput sequencing reveals divergent badnavirus species
To analyze the circular DNA virome from badnavirus-infected sugarcane ( Saccharum spp.) accession materials, viral genomes were amplified via rolling...
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Impacts of florfenicol on soil bacterial community structure and diversity by high throughput sequencing analysis
To investigate the impact of florfenicol on soil microbial community structure and diversity, an indoor florfenicol exposure model was established....
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High-throughput sequencing in plant disease management: a comprehensive review of benefits, challenges, and future perspectives
High-throughput sequencing (HTS) has instigated a paradigm shift in plant pathology, showcasing its transformative role in the management of plant...
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Analysis of Kefir Grains from Different Regions of the Planet Using High-Throughput Sequencing
AbstractThe taxonomic composition and spatial localization of yeast and bacteria in kefir grains (KG) obtained for study from different regions of...
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Uncovering the potential molecular mechanism of liraglutide to alleviate the effects of high glucose on myoblasts based on high-throughput transcriptome sequencing technique
BackgroundMyoblasts play an important role in muscle growth and repair, but the high glucose environment severely affects their function. The purpose...
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Identification and characterization of 29 microsatellite loci for Tripneustes gratilla based on high-throughput sequencing
The sea urchin Tripneustes gratilla holds substantial ecological and economic importance in tropical marine ecosystems. To better understand the...
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Effects of tissue type and season on the detection of regulated sugarcane viruses by high throughput sequencing
High throughput sequencing (HTS) can supplement and may replace diagnostic tests for plant pathogens. However, the methodology and processing of HTS...
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Finger Millet Transcriptome Analysis Using High Throughput Sequencing Technologies
During the past two decades, high-throughput transcriptome sequencing has developed into a well-established and widely used research method....
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High-Throughput Sequencing Technologies in Metagenomics: Advanced Approaches for Algal Research
The fields of biology and environmental research have been shaped by high-throughput sequencing (HTS) technologies in the past few years. The study...
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Virome analysis of sweetpotato in three Brazilian regions using high-throughput sequencing
Sweetpotato ( Ipomoea batatas (L.) Lam.) is a highly nutritious food crop that suffers severe yield losses due to viral diseases. These are...
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High-Throughput Sequencing Technologies
Optical and biochemical methods determine the sequence of nucleotide bases in a DNA macromolecule. Since 2010, considerable progress has been made on...
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Applications of High-Throughput Phenotypic Phenomics
Because of the variety of genetic components, plants are affected by complex genetic-environment-management interactions that drive phenotypic...
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