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Epithelial Cell Culture Methods and Protocols
Back Cover Copy
This second edition volume expands on the previous edition with in-depth discussions on the rapid advancements in epithelial cell...
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Primary Human Nasal Epithelial Cell Culture
Primary human nasal epithelial cell (HNEC) cultures serve as an excellent model in studying physiology, disease, and therapy related to the...
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Amniotic Membrane and Amniotic Epithelial Cell Culture
Amniotic membrane (AM) is considered an important medical device for applications in regenerative medicine. The therapeutic properties of AM are due...
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Epithelial Stem Cell Regeneration in Acute and Chronic Airway Injury
Airway epithelial stem cells are critical to maintaining airway homeostasis, especially after injury and illness. The loss of intact epithelium in...
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Zeb1 facilitates corneal epithelial wound healing by maintaining corneal epithelial cell viability and mobility
The cornea is the outmost ocular tissue and plays an important role in protecting the eye from environmental insults. Corneal epithelial wounding...
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Fibroblast-Epithelium Co-culture Methods Using Epithelial Organoids and Cell Line–Derived Spheroids
Fibroblasts are an integral cell type of mammary gland stroma, which plays crucial roles in development, homeostasis, and tumorigenesis of mammary...
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Epithelial Stem Cell Regeneration in Acute and Chronic Airway Injury
Airway epithelial stem cells are critical to maintaining airway homeostasis, especially after injury and illness. The loss of intact epithelium in...
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Epithelial Mechanosensing at Cell-Cell Contacts and Tight Junctions
This chapter focuses on the mechanosensitive properties of epithelial tissues. Epithelia experience a range of mechanical forces arising both...
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CSE reduces OTUD4 triggering lung epithelial cell apoptosis via PAI-1 degradation
Ovarian tumor family deubiquitinase 4 (OTUD4), a member of the OTU deubiquitinating enzyme, is implicated to decrease in cancer to regulate cell...
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DPSCs regulate epithelial-T cell interactions in oral submucous fibrosis
BackgroundOral submucous fibrosis (OSF) is a precancerous lesion characterized by fibrous tissue deposition, the incidence of which correlates...
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Germ cell-specific gene 2 accelerates cell cycle in epithelial ovarian cancer by inhibiting GSK3α-p27 cascade
Epithelial ovarian cancer (EOC) is one of the most common malignant gynecological tumors with rapid growth potential and poor prognosis, however, the...
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Foxo3 regulates cortical and medullary thymic epithelial cell homeostasis with implications in T cell development
Within the thymus, thymic epithelial cells (TECs) create dedicated microenvironments for T cell development and selection. Considering that TECs are...
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Death receptor 5 is required for intestinal stem cell activity during intestinal epithelial renewal at homoeostasis
Intestinal epithelial renewal, which depends on the proliferation and differentiation of intestinal stem cells (ISCs), is essential for epithelial...
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Single-cell transcriptome analysis of epithelial, immune, and stromal signatures and interactions in human ovarian cancer
A comprehensive investigation of ovarian cancer (OC) progression at the single-cell level is crucial for enhancing our understanding of the disease,...
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Establishment of primary prostate epithelial and tumorigenic cell lines using a non-viral immortalization approach
BackgroundResearch on prostate cancer is mostly performed using cell lines derived from metastatic disease, not reflecting stages of tumor initiation...
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Mitigation of acute lung injury by human bronchial epithelial cell-derived extracellular vesicles via ANXA1-mediated FPR signaling
Acute lung injury (ALI) is characterized by respiratory failure resulting from the disruption of the epithelial and endothelial barriers as well as...
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Dynamics and functions of E-cadherin complexes in epithelial cell and tissue morphogenesis
Cell–cell adhesion is at the center of structure and dynamics of epithelial tissue. E-cadherin–catenin complexes mediate Ca 2+ -dependent trans- homodime...
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Culture of Mouse Thymic Epithelial Cells in Serum-Free Medium
Primary cell culture systems are widely used as a valuable method for analyzing the biological functions of specific cells in vitro. Recently,...
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SMOC2 promotes an epithelial-mesenchymal transition and a pro-metastatic phenotype in epithelial cells of renal cell carcinoma origin
Renal Cell Carcinoma (RCC) is the most common form of all renal cancer cases, and well-known for its highly aggressive metastatic behavior. SMOC2 is...
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Engineering Epithelial–Mesenchymal Microtissues to Study Cell–Cell Interactions in Development
Intercellular signaling drives human development, but there is a paucity of in vitro models that recapitulate important tissue architecture while...