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Spin Labeling of Long RNAs Via Click Reaction and Enzymatic Ligation
Electron paramagnetic resonance (EPR) is a spectroscopic method for investigating structures, conformational changes, and dynamics of...
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13C pulse labeling indicates differing dynamics for two pools of amino acids in ectomycorrhizal fungi
Background and aimsEctomycorrhizal fungi synthesize carbohydrates and amino acids from recent photosynthate but can also assimilate soil-derived...
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Stable Isotope Labeling and Quantification of Photosynthetic Metabolites
Stable isotope labeling with 13CO2 coupled with mass spectrometry allows monitoring the incorporation of 13C into photosynthetic intermediates and is...
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Quantification of absolute labeling efficiency at the single-protein level
State-of-the-art super-resolution microscopy allows researchers to spatially resolve single proteins in dense clusters. However, accurate...
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Super-resolution proximity labeling with enhanced direct identification of biotinylation sites
Promiscuous labeling enzymes, such as APEX2 or TurboID, are commonly used in in situ biotinylation studies of subcellular proteomes or...
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Enzymatic Sequencing of Heparin Oligosaccharides Using Exolyase
Heparin/heparan sulfate (HP/HS) is a class of acidic polysaccharides with many potential medical applications, especially HP, and its derivatives,...
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Post-click labeling enables highly accurate single cell analyses of glucose uptake ex vivo and in vivo
Cellular glucose uptake is a key feature reflecting metabolic demand of cells in physiopathological conditions. Fluorophore-conjugated sugar...
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Engineered allostery in light-regulated LOV-Turbo enables precise spatiotemporal control of proximity labeling in living cells
The incorporation of light-responsive domains into engineered proteins has enabled control of protein localization, interactions and function with...
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DNA Labeling Using DNA Methyltransferases
DNA methyltransferases (MTases) uniquely combine the ability to recognize and covalently modify specific target sequences in DNA using the ubiquitous...
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Quantitation of MUC5AC and MUC5B by Stable Isotope Labeling Mass Spectrometry
Mucins MUC5AC and MUC5B are large glycoproteins that play an essential role in the innate defense of epithelial surfaces and their quantitation in...
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Carbon allocation in an East African ant-acacia: field testing a 13C-labeling method for evaluating biotic impacts on the carbon cycle
Tree carbon allocation is a dynamic process that depends on the tree’s environment, but we know relatively little about how biotic interactions...
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Proximity labeling reveals a new in vivo network of interactors for the histone demethylase KDM5
BackgroundKDM5 family proteins are multi-domain regulators of transcription that when dysregulated contribute to cancer and intellectual disability....
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Chemical Dual End-Labeling of Large Ribozymes
Fast and efficient site-specific labeling of long RNAs is one of the main bottlenecks limiting distance measurements by means of Förster resonance...
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Isolation and Immunodetection of Enzymatic DNA–Protein Crosslinks by RADAR Assay
DNA–protein crosslinks (DPCs) are steric hindrances to DNA metabolic processes and the removal and repair of DPCs is a rapidly evolving area of...
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The gluconeogenesis enzyme PCK2 has a non-enzymatic role in proteostasis in endothelial cells
Endothelial cells (ECs) are highly glycolytic, but whether they generate glycolytic intermediates via gluconeogenesis (GNG) in glucose-deprived...
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Fractionation of selenium isotopes during biofortification of Saccharomyces cerevisiae and the influence of metabolic labeling with 15N
Isotope fractionation of metals/metalloids in biological systems is an emerging research area that demands the application of state-of-the-art...
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Comparing the mesenchymal stem cells proliferation rate with different labeling assessments
Adipose tissue-derived mesenchymal stem cells (AD-MSCs) are an appropriate source for regenerative medicine and transplantation therapy due to their...
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Measuring the Oxidation State and Enzymatic Activity of Glyceraldehyde Phosphate Dehydrogenase (GAPDH)
Glyceraldehyde phosphate dehydrogenase (GAPDH) is a highly conserved, essential, and abundant enzyme that catalyzes a rate-determining step of...
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Synthesis of Fluorescent-Labeled Nucleotides for Labeling of Isothermal Amplification Products
AbstractFluorescently labeled nucleotides that contain a zwitterionic indodicarbocyanine dye have been synthesized. The dye has been attached at the...
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Fluorescent labeling of abundant reactive entities (FLARE) for cleared-tissue and super-resolution microscopy
Fluorescence microscopy is a vital tool in biomedical research but faces considerable challenges in achieving uniform or bright labeling. For...
