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Functional Screening of cDNA Expression Library for Novel Ctenophore Photoproteins
The functional screening of cDNA libraries (or functional cloning) enables isolation of cDNA genes encoding novel proteins with unknown amino acid...
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Rapid Amplification of cDNA Ends (RACE)
Rapid amplification of cDNA ends (RACE) or “one-sided” polymerase chain reaction (PCR) or “anchored” PCR is a polymerase chain reaction-based...
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cDNA-detector: detection and removal of cDNA contamination in DNA sequencing libraries
BackgroundExogenous cDNA introduced into an experimental system, either intentionally or accidentally, can appear as added read coverage over that...
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Sanger Sequencing to Determine the Full-Length Sequence of Circular RNAs
The pre-existing theory of pre-mRNA splicing into linear mature RNA was questioned with the introduction of circular RNAs (circRNAs). Hundreds of...
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High-throughput RNA isoform sequencing using programmed cDNA concatenation
Full-length RNA-sequencing methods using long-read technologies can capture complete transcript isoforms, but their throughput is limited. We...
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Genetic diversity of apple fruit crinkle viroid populations in Japanese persimmons and the infectivity of a predominant sequence variant to tomato plants
Apple fruit crinkle viroid (AFCVd) infects apple, hop, and oriental persimmon plants as natural hosts. The genetic diversity of AFCVd populations in...
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Nano3P-seq: transcriptome-wide analysis of gene expression and tail dynamics using end-capture nanopore cDNA sequencing
RNA polyadenylation plays a central role in RNA maturation, fate, and stability. In response to developmental cues, polyA tail lengths can vary,...
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The Bread Wheat Reference Genome Sequence
In 2018, the International Wheat Genome Sequencing Consortium published a reference genome sequence for bread wheat (Triticum aestivum L.). The...
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Molecular characteristics and pathogenicity of infectious cDNA clones of the Chinese isolates of pea enation mosaic virus 1 and pea enation mosaic virus 2
The pea enation mosaic disease (PEMD) causes significant yield losses worldwide. PEMD is caused by two taxonomically unrelated but symbiotic viruses,...
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The Efficiency of Various DNA Polymerases for Amplification of Long Sequences from Genomic DNA and cDNA of Cultivated Potatoes
AbstractAmplification of long fragments from complex templates, such as eukaryotic genomic DNA, is considered a difficult task for most DNA...
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Molecular cloning and characterisation of the PmEglin cDNA in the leech Hirudinaria sp.
The potato type I proteinase inhibitor family is a group of serine proteases that can alter physiological processes by regulating immune responses,...
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Molecular Cloning of a cDNA for Chrysanthemum morifolium Ramat Squalene Synthase Gene and Impact of Its Silencing on Transgenic Chrysanthemum Plants
AbstractThe aim of this research was to study the impact of squalene synthase gene silencing on the features of transgenic chrysanthemum plants, and...
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Biolistic Inoculation of Fruit Trees with Full-Length Infectious cDNA Clones of RNA Viruses
Long life cycle and lack of efficient and robust virus inoculation technique are the major technical challenges for studying virus infection in...
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The nearly complete genome sequence of clade B, wheat streak mosaic virus isolate from Türkiye
Wheat streak mosaic virus is one of the most widespread viruses in cereal crops, causing severe losses and dramatically affecting worldwide wheat...
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Reference-aided full-length transcript assembly, cDNA cloning, and molecular characterization of coronatine-insensitive 1b (COI1b) gene in coconut (Cocos nucifera L.)
BackgroundIn the Philippines, 26% of the total agricultural land is devoted to coconut production making coconut one of the most valuable industrial...
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Complete genome sequence of eggplant mild leaf mottle virus (EMLMV) infecting eggplant in India
The complete nucleotide sequence of eggplant mild leaf mottle virus (EMLMV), of the genus Ipomovirus identified from the leaves of eggplants of Uttar...
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Complete genomic sequence of turnip mosaic virus infecting passionfruit in Fujian province of China
Contigs with sequence identities to turnip mosaic virus (TuMV) were detected by deep sequencing of the small RNAs extracted from a passion fruit ( Pass...
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Fructokinases in potato (Solanum tuberosum L.): molecular cloning, sequence analyses, three-dimensional structure prediction, enzyme activity and purification by aqueous two-phase system
Fructokinase (FRK) (ATP: D -fructose 6-phosphotransferase; EC 2.7.1.4) is a key enzyme in fructose metabolism in the sink organs of plants. In...
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cDNA cloning and expression analysis of glutaredoxin 3 in black tiger shrimp Penaeus monodon
Glutathione (Grx) is a glutathione-dependent oxidoreductase belonging to the thioredoxin (Trx) superfamily. It maintains redox homeostasis in...
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Agroinoculation of a full-length cDNA clone of citrus yellow vein clearing virus results in systemic infection in Nicotiana benthamiana and induces disturbances of subcellular structure
Citrus yellow vein clearing virus (CYVCV) is the causal agent of citrus yellow vein clearing disease. In the present study, we performed...
