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Asymmetric Contribution of Blastomere Lineages of First Division of the Zygote to Entire Human Body Using Post-Zygotic Variants
Background:In humans, after fertilization, the zygote divides into two 2n diploid daughter blastomeres. During this division, DNA is replicated, and...
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FUS driven circCNOT6L biogenesis in mouse and human spermatozoa supports zygote development
Circular RNA (circRNA) biogenesis requires a backsplicing reaction, promoted by inverted repeats in cis -flanking sequences and trans factors, such as...
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Dissecting the Multiple Functions of the Polo-Like Kinase 1 in the C. elegans Zygote
Plk1 (polo-like kinase 1) is an evolutionarily conserved serine/threonine kinase instrumental for mitotic entry and progression. Beyond these...
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Electroporation-Mediated CRISPR/Cas9 Genome Editing in Rat Zygotes
Genetic engineering in the rat has been revolutionized by the development of CRISPR-based genome editing tools. Conventional methods for inserting...
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Generation of Knock-In Mouse by Genome Editing
Knock-in mice are useful for evaluating endogenous gene expressions and functions in vivo. Instead of the conventional gene-targeting method using...
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Somatic embryogenesis induction in Rosa persica Michx ex Juss.
The current research aimed to evaluate the impact of various explants (immature zygote embryos, hypocotyls, and cotyledons), the effects of liquid...
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An efficient semi–in vivo zygotic embryogenesis system in Arabidopsis
Embryogenesis is the critical developmental phase in the life cycle of flowering plants, which starts with the double fertilization. In the model...
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DNA methylation remodeling and the functional implication during male gametogenesis in rice
BackgroundEpigenetic marks are reprogrammed during sexual reproduction. In flowering plants, DNA methylation is only partially remodeled in the...
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Efficient CRISPR/Cas9-Assisted Knockin of Large DNA Donors by Pronuclear Microinjection During S-Phase in Mouse Zygotes
In the CRISPR/Cas9-mediated gene cassette knockin (KI) strategy, a gene cassette is integrated into a target locus through a proper DNA repair...
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Pivotal role for long noncoding RNAs in zygotic genome activation in mice
Vertebrate life begins with fertilization, and then the zygote genome is activated after transient silencing, a process termed zygotic genome...
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Expression Patterns and Biological Roles of Micall2a during Zebrafish Vascular Development
AbstractMICAL-like 2 belongs to a multi-domain protein family and involves in a series of biological processes. However, the expression patterns and...
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Distinctive development of embryo and endosperm caused by male gametes irradiated with carbon-ion beam
Key messageIn Cyrtanthus mackenii , development of embryo and endosperm were differentially affected by fertilization of male gametes with DNA damage...
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Fortpflanzung und Entwicklung
Bei der Fortpflanzung verschmilzt ein haploides Spermium mit einer haploiden Eizelle zur diploiden Zygote. Spermium und Eizelle sind...
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Donor template delivery by recombinant adeno-associated virus for the production of knock-in mice
BackgroundThe ability of recombinant adeno-associated virus to transduce preimplantation mouse embryos has led to the use of this delivery method for...
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Embryogenesis and early larval development in bocachico (Prochilodus magdalenae) in Colombia
BackgroundThe bocachico ( Prochilodus magdalenae ) is a migratory freshwater fish and one of the main species of Colombian continental fishery....
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The roles of a novel CDKB/KRP/FB3 cell cycle core complex in rice gametes and initiation of embryogenesis
The cell cycle controls division and proliferation of all eukaryotic cells and is tightly regulated at multiple checkpoints by complexes of core cell...
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Embryo Development of Dendrobium officinale in Relation to Seed Germination In Vitro
AbstractIn this study, we focus on the zygote and embryo development process and the seed germination system in vitro of Dendrobium officinale ....
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Production of gene-edited pigs harboring orthologous human mutations via double cutting by CRISPR/Cas9 with long single-stranded DNAs as homology-directed repair templates by zygote injection
Precise gene editing of model organisms is required for accurately modeling human diseases and deciphering gene functions. In this study, we used a...
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Electroporation of AsCpf1/RNP at the Zygote Stage is an Efficient Genome Editing Method to Generate Knock-Out Mice Deficient in Leukemia Inhibitory Factor
BACKROUND: CRISPR/Cpf1 is a class II, type V RNA-guided endonuclease that is distinct from the type II CRISPR/Cas9 nuclease, widely used for genome...
