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Protein–Protein Interactions: Yeast Two Hybrid
The yeast two-hybridYeast two-hybrid (Y2H) systemTwo-hybrid system is a powerful and commonly used genetic tool to investigate the interaction...
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Yeast Two-Hybrid Technique to Identify Protein–Protein Interactions
Protein–protein interactions are specific and direct physical contact between two or more proteins, and the interaction involves hydrogen bonding,...
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Next-Generation Yeast Two-Hybrid Screening to Discover Protein–Protein Interactions
Yeast two-hybrid is a powerful approach to discover new protein–protein interactions. Traditional methods involve screening a target protein against...
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Recent progress on heterologous protein production in methylotrophic yeast systems
Recombinant protein production technology is widely applied to the manufacture of biologics used as drug substances and industrial proteins such as...
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High-Throughput Protein–Protein Interactions Screening Using Pool-Based Liquid Yeast Two-Hybrid Pipeline
Because of its adaptability to high-throughput approaches and a low operating cost, the yeast two-hybrid (Y2H) assay remains the most widely used one...
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Detection of Protein–Protein Interactions Utilizing the Split-Ubiquitin Membrane-Based Yeast Two-Hybrid System
Identifying the interactors of a protein is a key step in understanding its possible cellular function(s). Among the various methods that can be used...
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The Promising Role of Selenium and Yeast in the Fight Against Protein Amyloidosis
In recent years, increasing attention has been paid to research on diseases related to the deposition of misfolded proteins (amyloids) in various...
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Yeast Surface Display
This detailed volume explores a wide variety of applications of yeast surface display, an extensively used protein engineering technology. Beginning...
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Lager brewing yeast
Lager brewing yeast is a group of closely related strains of Saccharomyces pastorianus/S. carlsbergensis used for lager beer production all over the...
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Accessing Transient Binding Pockets by Protein Engineering and Yeast Surface Display Screening
The binding pocket of some therapeutic targets can acquire multiple conformations that, to some extent, depend on the protein dynamics and the...
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Rewiring of the protein–protein–metabolite interactome during the diauxic shift in yeast
In budding yeast Saccharomyces cerevisiae , the switch from aerobic fermentation to respiratory growth is separated by a period of growth arrest,...
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Ectopic BH3-Only Protein Bim Associates with Hsp70 to Regulate Yeast Mitophagy
AbstractMitophagy, a form of selective autophagy, plays an essential role to maintain a population of healthy and functional mitochondria for normal...
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Quantification of Golgi Protein Mislocalization to the Budding Yeast Vacuole
The localization of proteins to the Golgi complex is a dynamic process requiring sorting signals in the cytosolic domains of resident Golgi proteins...
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Production, Economics, and Marketing of Yeast Single Cell Protein
Increase in the world population living below the poverty line actuates the scientific community to find economic alternative to conventional...
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Enhanced Yeast One-Hybrid Assays to Study Protein–DNA Interactions
The specificity in gene regulation is controlled by interactions between transcription factors (TFs) and genomic DNA regions such as promoters and...
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Employing protein size in the functional analysis of orthologous proteins, as illustrated with the yeast HOG pathway
Comparative genomics has provided us with a new handle on the interpretation of protein sequences. The sequencing of numerous fungal genomes has...
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Membrane Protein Production in the Yeast P. pastoris
The first crystal structures of recombinant mammalian membrane proteins were solved using high-quality protein that had been produced in yeast cells....
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The serine-arginine (SR) protein UmRrm75 from Ustilago maydis is a functional ortholog of yeast ScHrb1
The Basidiomycete fungus Ustilago maydis is a biotrophic pathogen of maize. The U. maydis UmRrm75 gene encodes an RNA-binding protein (RBP). In a...
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12 Baker’s Yeast: a rising foundation for eukaryotic sphingolipid-mediated cell signaling
Saccharomyces cerevisiae has been an invaluable tool for the dissection of sphingolipid metabolic pathways and cloning of enzymes involved in...
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Fus3 and Tpk2 protein kinases regulate the phosphorylation-dependent functions of RNA helicase Dhh1 in yeast mating and Ste12 protein expression
Decap** of mRNA is a key regulatory step for mRNA decay and translation. The RNA helicase, Dhh1, is known as a decap** activator and translation...